Recombinant Human Transcription elongation factor SPT6 (SUPT6H), partial

中文名稱：

人SUPT6H重組蛋白
貨號：

CSB-YP752095HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人SUPT6H重組蛋白
貨號：

CSB-EP752095HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人SUPT6H重組蛋白
貨號：

CSB-EP752095HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人SUPT6H重組蛋白
貨號：

CSB-BP752095HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人SUPT6H重組蛋白
貨號：

CSB-MP752095HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SUPT6H
Uniprot No.：

Q7KZ85
別名：

emb 5; hSPT6; KIAA0162; MGC87943; SPT6H; SPT6H_HUMAN; Suppressor of Ty (S.cerevisiae) 6 homolog; Suppressor of Ty 6 homolog (S. cerevisiae); SUPT6H; Tat cotransactivator 2 protein; Tat CT2 protein; Tat-cotransactivator 2 protein; Tat-CT2 protein; Transcription elongation factor SPT6
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.
基因功能參考文獻：
1. Altogether, these results indicate that a complex containing LEDGF/p75, Iws1, and Spt6 participates in regulating postintegration steps of HIV latency. PMID: 25590759
2. identified fibronectin, a component of the extracellular matrix and a mesenchymal marker, as a transcriptional target of Pak1 signaling PMID: 24441044
3. Spt6 is a unique histone chaperone capable of regulating the histone epigenetic state of both AID targets and the AID locus. PMID: 22843687
4. These results show that intracellular levels of Spt6 are fine-tuned by PAAF1 and proteasome, which is required for HIV-1 transcription and extends to cellular genes implicated in cancer. PMID: 22316138
5. Abolishing UL69's ability to interact with the SPT6 protein inhibited human cytomegalovirus replication. PMID: 22171252
6. Knockdown of Spt6 resulted in severe reduction of class switch recombination in the endogenous Ig locus in B cells. PMID: 21518874
7. Reults establish that human Spt6 (hSpt6) is a classic transcription elongation factor that enhances the rate of RNA polymerase II elongation. PMID: 15060154
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亞細胞定位：

Nucleus.
蛋白家族：

SPT6 family
組織特異性：

Ubiquitously expressed.
數據庫鏈接：

HGNC: 11470

OMIM: 601333

KEGG: hsa:6830

STRING: 9606.ENSP00000319104

UniGene: Hs.250429