Recombinant Human T-cell surface glycoprotein CD8 alpha chain (CD8A), partial
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中文名稱：Recombinant Human T-cell surface glycoprotein CD8 alpha chain(CD8A),partial
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貨號：CSB-MP004966HU
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說明書：
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規格：￥1140
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圖片：
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. -
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP004966HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD8A. -
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP004966HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD8A.
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其他：
產品詳情
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純度：Greater than 90% as determined by SDS-PAGE.
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基因名：
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Uniprot No.：
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別名：alpha polypeptide (p32); CD8; CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide (p32); CD8a; CD8A antigen; CD8A molecule; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; Ly3; LYT3; MAL; OKT8 T cell antigen; OTTHUMP00000160760; OTTHUMP00000160764; OTTHUMP00000203528; OTTHUMP00000203721; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen; T8/Leu-2 T-lymphocyte differentiation antigen
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種屬：Homo sapiens (Human)
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蛋白長度：Partial
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來源：Mammalian cell
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分子量：21.6kDa
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表達區域：22-182aa
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氨基酸序列SQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering. -
蛋白標簽：N-terminal 6xHis-Myc-tagged
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產品提供形式：Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. -
儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：3-7 business days
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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產品描述：
The production of this Recombinant Human CD8A protein required the insertion of a DNA fragment (CD8A, 22-182aa) into a plasmid vector and the transferral of this vector into Mammalian cell cells (the step of transformation). The cells were then cultured and induced to express the CD8A protein. This recombinant protein was fused with N-terminal 6xHis tag. Its purity is 90%+ determined by SDS-PAGE.
CD8A(also called MAL) is a gene encoding a protein named T-cell surface glycoprotein CD8 alpha chain (also known as T-lymphocyte differentiation antigen T8/Leu-2 or CD8a) in human. CD8a protein can form homodimers and heterodimers with CD8a and CD8β, respectively. Both homodimers and heterodimers are CD8 protein, which is a co-receptor for T cell receptor when it interacts with MHC class I–peptide complex. the CD8a is required for expression of both dimers on the cell surface. Emerging evidence has revealed that CD8A gene is involved in coronavirus biology, and is relevant to COVID-19 prognosis.
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Datasheet & COA：Please contact us to get it.
產品評價
相關產品
靶點詳情
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功能：Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
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基因功能參考文獻：
- Studied lymphocyte phenotype in resected lymph nodes of patients with lung cancer by analyzing levels of Foxp3 and CD8 by immunohistochemical staining. PMID: 28831395
- This study compares the differences of PD-L1 expression and CD8+ tumor-infiltrating lymphocyte density, two major response biomarkers of PD-1/PD-L1 blockade, between paired primary and brain metastatic lesions in advanced non-small cell lung cancer (NSCLC). For NSCLC brain metastases patients, CD8+ tumor-infiltrating lymphocyte density showed a spatial and temporal heterogeneity. PMID: 29526752
- Endometrioid endometrial carcinomas are capable of down-regulating CD8 expression by cytotoxic T lymphocytes. PMID: 27346574
- The PD-1/CD8 ratio may, therefore, be a useful prognostic marker for stage II/III CRC. What is important for predicting the prognosis may be the PD-1/CD8 ratio rather than the absolute number of PD-1(+) tumor-infiltrating lymphocytes . PMID: 28739701
- We also observed a significant association between forkhead box protein 3 ( p = 0.001) and the Neo-Bioscore, while only a marginal difference was observed with CD8+ expression ( p = 0.074). This study demonstrated that forkhead box protein 3 expression has value as the only independent marker that predicts a good response to neoadjuvant chemotherapy and that it is related with a good prognosis according to the Neo-Biosc PMID: 28621227
- missense mutation increases susceptibility to recurrent respiratory infections PMID: 26563160
- Human mesenchymal stromal cells enhance the immunomodulatory function of CD8(+)CD28(-) regulatory T cells. PMID: 25482073
- Dendritic cells accumulate in the bone marrow of multiple myeloma patients and protect tumor plasma cells from CD8+ T-cell killing. PMID: 26185130
- Patients with the presence of CD8- and CD45RO-positive T cells in bone marrow demonstrated better survival of gastric cancer patients than those with the absence of these cells in bone marrow. PMID: 25804232
- More sCD8 circulating molecules were found in patients transfused with post-storage leucodepleted RBCs whose supernatants had large amounts of sHLA-I. sCD8 is a possible modulator of sHLA-I-mediated transfusion-related immunomodulation. PMID: 23356971
- CD8-positivity proved to be an independent prognostic predictor of worse outcome in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma. PMID: 23716548
- CREMalpha orchestrates epigenetic remodeling of the CD8A,B through the recruitment of DNA methyltransferase (DNMT) 3a and histone methyltransferase G9a. PMID: 24297179
- Modified CD8A gene function may contribute to the development of refractory chronic rhinosinusitis via reduction of circulating CD8 lymphocytes. PMID: 23640800
- transcription factor cAMP-responsive element modulator alpha (CREMalpha), which is expressed at increased levels in T cells from systemic lupus erythematosus patients, contributes to transcriptional silencing of CD8A and CD8B. PMID: 24047902
- The CD8(+)CCR7(+) T-cell frequency in HNSCC patients' blood tested at diagnosis can discriminate them from normal controls and predicts disease recurrence PMID: 23363813
- The expression of human leucocyte antigen (HLA)-DR in epithelial cells and cluster of differentiation (CD8)-positive lymphocytes as possible markers of chronic ocular graft versus host disease, was investigated. PMID: 23878500
- HIV-1 clade-B Nef peptides elicit increased frequency, breadth and function of CD8+ T-cells compared to consensus peptides PMID: 23185362
- analysis of the effector phenotype of CD8+ T cells at the junction epithelium during clinical quiescence of herpes simplex virus 2 infection PMID: 22811543
- study confirms that CD8 expression is a recurrent albeit rare phenomenon in patients with chronic lymphocytic leukemia and suggests that CD8 expression has an adverse prognostic impact PMID: 22143984
- Silencing Hsp25/Hsp27 gene expression augments proteasome activity and increases CD8+ T-cell-mediated tumor killing and memory responses PMID: 22185976
- LNX1 and LNX2 interact with CD8alpha and promote its ubiquitylation and endocytosis. PMID: 22045731
- Nef-mediated down-modulation of CD8alphabeta is a fundamental property of primate lentiviruses. PMID: 22013062
- Multiple sclerosis is characterized by a dysregulation of CD8+CD56-perforin+ T cells that may play a role in the development of disability. PMID: 22001684
- Lymphoid tissue (LT)-resident CD8alpha-positive dendritic cells (DCs) and non(LT)-derived CD103-positive DCs express transgenic XCR1 and are characterized by a unique transcriptional fingerprint, irrespective of their tissue of origin. PMID: 21948982
- Coreceptor CD8alphabeta contributes to the antigen-recognition process by binding to a largely invariant region of the MHC class I molecule and by promoting intracellular signaling. PMID: 21954283
- These results suggest that the elevated frequency of PD-1(+) CD8(+) T cells could be associated with leukemic relapse in pediatric umbilical cord blood transplantation recipients. PMID: 21813446
- The aim was to determine if SPARC, FOXP3, CD8 and CD45RO expression levels are associated with colorectal cancer (CRC) stage, disease outcome and long-term cancer-specific survival PMID: 21818290
- we present the complex structures of human CD8alphaalpha bound to HLA-A*2402 PMID: 21645925
- the presence of IL-1 and/or IL-6 during activation of human CD8 T cells attenuates Fas-mediated AICD, whereas IL-12 increases the susceptibility of activated CD8 T cells to this form of cell death PMID: 21518761
- In the setting of HIV-induced lymphopenia, naive CD4-expressing T cells are recruited mainly into the proliferating pool in response to CD4 T cell depletion, whereas naive CD8-positive T cell proliferation is driven mainly by levels of HIV RNA. PMID: 21257970
- Studies indicate that The thymus leukemia (TL) antigen and CD8alphaalpha are interacting surface molecules that are expressed at the frontline of the mucosal immune system. PMID: 20850477
- PD-L1 and PD-L2 knockdown dendritic cells showed superior potential to expand minor histocompatibility antigen-specific CD8(+) effector and memory T cells from leukemia patients early after donor lymphocyte infusion and later during relapse. PMID: 20682852
- The study shows that impaired Epstein-Barr virus-specific CD8+ T-cell function in X-linked lymphoproliferative disease is restricted to SLAM family-positive B-cell targets. PMID: 20644117
- The positive rate of CD8 in primary lung cancer tissue was significantly higher than that in normal lung tissue. PMID: 20704820
- Binding of the CD8 coreceptor to class I histocompatibility molecules significantly enhances the amount of calcium released from intracellular stores, triggered by a weaker ESO1 peptide agonist. PMID: 20053942
- Four new weak but tissue-specific DNase I hypersensitivity sites have been mapped 0-0.8 kb upstream of the first exon of CD8A, in the promoter region. PMID: 11937547
- Soluble HLA-A,-B,-C and -G molecules induce apoptosis in T and NK CD8+ cells and inhibit cytotoxic T cell activity through CD8 ligation. PMID: 12594841
- expression of the HMG box protein TOX is sufficient to induce changes in coreceptor gene expression associated with beta-selection, including CD8 gene demethylation PMID: 15078895
- Establishment of a permanent CD8+ cell line from a patient with leukemic phase of acute lymphoblastic lymphoma. PMID: 15927673
- p32 recruits ganciclovir kinase to redistribute the nuclear lamina. PMID: 15975922
- Introduction of CD8 alpha down-regulates the production of major T helper (Th) type 2 cytokines and may induce differentiation of tumor-specific Th1 cells to improve the clinical potential of T cell receptor (TCR) gene transfer to treat melanoma. PMID: 16818755
- The results indicate that p.Gly111Ser is confined to the Spanish Gypsy population, where it occurs at a carrier rate of 0.4%. PMID: 17658607
- human monocytes express CD8 alpha; co-engagement of CD8 alpha and FcR enhances monocyte TNF release, suggesting FcR may be a novel partner receptor for CD8 alpha on innate immune cells PMID: 17678538
- CD8+FOXP3+ T cells play an important role in unresponsiveness related to upregulated interleukin receptors on dendritic cells. PMID: 18089323
- Data suggest that Bim-mediated attrition of HBV-specific CD8(+) T cells contributes to the inability of these cell populations to persist and control viral replication. PMID: 18398508
- tumor-induced TGF-beta may actively subvert the CD8+ arm of the immune system into directly promoting tumor growth by an IL-17-dependent mechanism PMID: 18483277
- Data suggest that a decrease in CD8+CD28- T cells may reflect impaired T-cell suppression and accordingly, increased T cell help to autoreactive B cells in patients with systemic lupus erythematosus. PMID: 18625635
- analysis of an interaction between B7-H3 and TLT-2 that preferentially enhances CD8(+) T cell activation PMID: 18650384
- polarized CD8 T cells within the HBV-infected liver may impede proliferative antiviral effector function, while contributing to the proinflammatory cytokine environment PMID: 18695005
- in patients with melanoma, who underwent a peptide-based vaccination, homodimer may represent a marker of antigen-specific T-cell memory PMID: 18833003
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相關疾病：CD8 deficiency, familial (CD8 deficiency)
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亞細胞定位：[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
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組織特異性：CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and
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