Recombinant Human Separin (ESPL1), partial

中文名稱：

人ESPL1重組蛋白
貨號：

CSB-YP621785HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人ESPL1重組蛋白
貨號：

CSB-EP621785HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人ESPL1重組蛋白
貨號：

CSB-EP621785HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人ESPL1重組蛋白
貨號：

CSB-BP621785HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人ESPL1重組蛋白
貨號：

CSB-MP621785HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ESPL1
Uniprot No.：

Q14674
別名：

Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; SSE
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
基因功能參考文獻：
1. Separase activity measurement may therefore be useful as a novel additional molecular marker for disease monitoring PMID: 29370237
2. Studies identified and characterized the role of separase in mitosis, meiosis, non-canonical roles, its regulation, as a regulator of centriole disengagement, non proteolytic roles, diverse substrates, structural insights, and association of separase with cancer. [review] PMID: 27966791
3. High ESP expression is associated with breast cancer. PMID: 28859055
4. Proximity mapping of human separase has been presented. PMID: 27495871
5. The assay was used to quantify Separase proteolytic activity in leukemic cell lines and peripheral blood samples from leukemia patients. PMID: 26267133
6. Recruitment and activation of separase at centrosomes are two distinct steps that do not require microtubules. PMID: 25299182
7. Separase protein levels decrease and Separase proteolytic activity increases exclusively in b3a2 p210BCR-ABL-positive cell lines under Imatinib treatment. PMID: 26087013
8. Separase is an oncogene whose overexpression induces tumorigenesis, and indicate that Separase overexpression and aberrant nuclear localization are common in many tumor types and may predict outcome in some human malignancies. PMID: 24792645
9. High ESPL1 mRNA expression was associated with luminal B breast cancers. PMID: 25086634
10. Data indicate that separase is subject to native-state cis/trans isomerization by peptidyl-prolyl-isomerase Pin1. PMID: 25921067
11. By consecutively acting as a protease and a cdk1 inhibitor, separase coordinates two key processes to achieve simultaneous and abrupt separation of sister chromatids. PMID: 22814604
12. Kendrin is a novel and crucial substrate for separase (ESPL1) at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis. PMID: 22542101
13. Plk1-mediated phosphorylation of Cdc6 promotes the interaction of Cdc6 and Cdk1, leading to the attenuation of Cdk1 activity, release of separase, and subsequent anaphase progression. PMID: 21041660
14. Anaphase specific auto-cleavage of separase. PMID: 12297314
15. Processing, localization, and requirement of separase for normal anaphase progression. PMID: 12672959
16. Separase function is not restricted to anaphase initiation; its role in promoting loss of sister chromatid cohesion might be preferentially at arms but not centromeres. PMID: 16177575
17. Protein phosphatase 2A and separase form a complex regulated by separase autocleavage PMID: 17604273
18. Phosphorylation promotes complex formation indirectly, possibly by inducing a conformational change in full-length separase. PMID: 17974570
19. The complete removal of cohesin from chromosome arms depends on separase. PMID: 18003702
20. Separase has targets involved in regulation of G(2) to M progression after DNA damage in lung cancer cells. PMID: 18616699
21. These results collectively suggest that Separase is an oncogene, whose overexpression alone in mammary epithelial cells is sufficient to induce aneuploidy and tumorigenesis in a p53 mutant background. PMID: 18728194
22. Study reports that cohesin cleavage by human separase requires DNA in a sequence-nonspecific manner. PMID: 19345191
23. Separase might be an oncogene, whose overexpression induces tumorigenesis. Separase overexpression and aberrant nuclear localization are common in many tumor types and may predict outcome in some human cancers. PMID: 19351757
24. Plk1 and separase act at different times during M phase to license centrosome duplication, reminiscent of their roles in removing cohesin from chromosomes PMID: 19758559
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亞細胞定位：

Cytoplasm. Nucleus.
數據庫鏈接：

HGNC: 16856

OMIM: 604143

KEGG: hsa:9700

STRING: 9606.ENSP00000257934

UniGene: Hs.153479