Recombinant Human Ribosomal RNA small subunit methyltransferase NEP1 (EMG1)

中文名稱：

人EMG1重組蛋白
貨號：

CSB-YP852924HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人EMG1重組蛋白
貨號：

CSB-EP852924HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人EMG1重組蛋白
貨號：

CSB-EP852924HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人EMG1重組蛋白
貨號：

CSB-BP852924HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人EMG1重組蛋白
貨號：

CSB-MP852924HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

EMG1
Uniprot No.：

Q92979
別名：

18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA Psi1248 methyltransferase; C2F ; C2f protein; EMG 1; EMG1; EMG1 N1 specific pseudouridine methyltransferase; EMG1 nucleolar protein homolog (S cerevisiae); EMG1 nucleolar protein homolog; essential for mitotic growth 1; FLJ60792; Grcc2f ; NEP1 ; NEP1_HUMAN; Nucleolar protein EMG1 homolog; Protein C2f; Ribosomal RNA small subunit methyltransferase Nep1; Ribosome biogenesis protein NEP1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-244
氨基酸序列

AAPSDGFKP RERSGGEQAQ DWDALPPKRP RLGAGNKIGG RRLIVVLEGA SLETVKVGKT YELLNCDKHK SILLKNGRDP GEARPDITHQ SLLMLMDSPL NRAGLLQVYI HTQKNVLIEV NPQTRIPRTF DRFCGLMVQL LHKLSVRAAD GPQKLLKVIK NPVSDHFPVG CMKVGTSFSI PVVSDVRELV PSSDPIVFVV GAFAHGKVSV EYTEKMVSIS NYPLSAALTC AKLTTAFEEV WGVI
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.
基因功能參考文獻：
1. Our findings further indicate that in Bowen-Conradi syndrome, nuclear disassembly of the import complex and release of EMG1D86G lead to its nuclear aggregation and degradation, resulting in the reduced nucleolar recruitment of the RNA methyltransferase and defects in the biogenesis of the small ribosomal subunit. PMID: 27798105
2. Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome. PMID: 19463982
相關疾病：

Bowen-Conradi syndrome (BWCNS)
亞細胞定位：

Nucleus, nucleolus.
蛋白家族：

Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase NEP1 family
數據庫鏈接：

HGNC: 16912

OMIM: 211180

KEGG: hsa:10436

UniGene: Hs.558447