Recombinant Human Putative methyltransferase NSUN6 (NSUN6)

中文名稱：

人NSUN6重組蛋白
貨號：

CSB-YP823470HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人NSUN6重組蛋白
貨號：

CSB-EP823470HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人NSUN6重組蛋白
貨號：

CSB-EP823470HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人NSUN6重組蛋白
貨號：

CSB-BP823470HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人NSUN6重組蛋白
貨號：

CSB-MP823470HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

NSUN6
Uniprot No.：

Q8TEA1
別名：

4933414E04Rik; FLJ23743; NOL1/NOP2/Sun and PUA domain-containing protein 1; NOL1/NOP2/Sun domain family 6; NOL1/NOP2/Sun domain family member 6; NOP2; NOPD1; NSUN6; NSUN6_HUMAN; nucleolar protein (NOL1/NOP2/sun) and PUA domains 1; Putative methyltransferase NSUN6; Sun domain family; member 6
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-469
氨基酸序列

MSIFPKISLR PEVENYLKEG FMNKEIVTAL GKQEAERKFE TLLKHLSHPP SFTTVRVNTH LASVQHVKNL LLDELQKQFN GLSVPILQHP DLQDVLLIPV IGPRKNIKKQ QCEAIVGAQC GNAVLRGAHV YAPGIVSASQ FMKAGDVISV YSDIKGKCKK GAKEFDGTKV FLGNGISELS RKEIFSGLPE LKGMGIRMTE PVYLSPSFDS VLPRYLFLQN LPSALVSHVL NPQPGEKILD LCAAPGGKTT HIAALMHDQG EVIALDKIFN KVEKIKQNAL LLGLNSIRAF CFDGTKAVKL DMVEDTEGEP PFLPESFDRI LLDAPCSGMG QRPNMACTWS VKEVASYQPL QRKLFTAAVQ LLKPEGVLVY STCTITLAEN EEQVAWALTK FPCLQLQPQE PQIGGEGMRG AGLSCEQLKQ LQRFDPSAVP LPDTDMDSLR EARREDMLRL ANKDSIGFFI AKFVKCKST
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.
基因功能參考文獻：
1. The structures of NSun6, a human tRNA:m5C methyltransferase, in the apo form and in complex with a full-length tRNA substrate. PMID: 28531330
2. findings suggest that human NSun6 relies on a delicate network for RNA recognition, which involves both the primary sequence and tertiary structure of tRNA substrates. PMID: 27703015
3. Findings identified NSUN6 as a novel mammalian m5C tRNA methyltransferase and suggest that methylation of tRNAs by NSUN6 occurs during a late step of tRNA biogenesis, after the addition of the CCA tail and export of the tRNA from the nucleus. PMID: 26160102
4. Observational study of gene-disease association. (HuGE Navigator) PMID: 21044367
5. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
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亞細胞定位：

Cytoplasm.
蛋白家族：

Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family
數據庫鏈接：

HGNC: 23529

KEGG: hsa:221078

STRING: 9606.ENSP00000366519

UniGene: Hs.396175