Recombinant Human Protein phosphatase 1D (PPM1D)
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中文名稱：人PPM1D重組蛋白
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貨號：CSB-EP018491HU-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：EC 3.1.3.16; p53 induced protein phosphatase 1; p53-induced protein phosphatase 1; PP2C delta; PP2C-delta; PP2CD; Ppm1d; PPM1D_HUMAN; Protein phosphatase 1D; protein phosphatase 1D magnesium-dependent delta isoform ; Protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium dependent 1 delta; Protein phosphatase magnesium-dependent 1 delta; Protein phosphatase Mg2+/Mn2+ dependent 1D; Protein phosphatase Wip1; Wild type p53 induced phosphatase 1; WIP 1; WIP1
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種屬：Homo sapiens (Human)
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蛋白長度：Full length protein
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表達區域：1-605
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氨基酸序列MAGLYSLGVS VFSDQGGRKY MEDVTQIVVE PEPTAEEKPS PRRSLSQPLP PRPSPAALPG GEVSGKGPAV AAREARDPLP DAGASPAPSR CCRRRSSVAF FAVCDGHGGR EAAQFAREHL WGFIKKQKGF TSSEPAKVCA AIRKGFLACH LAMWKKLAEW PKTMTGLPST SGTTASVVII RGMKMYVAHV GDSGVVLGIQ DDPKDDFVRA VEVTQDHKPE LPKERERIEG LGGSVMNKSG VNRVVWKRPR LTHNGPVRRS TVIDQIPFLA VARALGDLWS YDFFSGEFVV SPEPDTSVHT LDPQKHKYII LGSDGLWNMI PPQDAISMCQ DQEEKKYLMG EHGQSCAKML VNRALGRWRQ RMLRADNTSA IVICISPEVD NQGNFTNEDE LYLNLTDSPS YNSQETCVMT PSPCSTPPVK SLEEDPWPRV NSKDHIPALV RSNAFSENFL EVSAEIAREN VQGVVIPSKD PEPLEENCAK ALTLRIHDSL NNSLPIGLVP TNSTNTVMDQ KNLKMSTPGQ MKAQEIERTP PTNFKRTLEE SNSGPLMKKH RRNGLSRSSG AQPASLPTTS QRKNSVKLTM RRRLRGQKKI GNPLLHQHRK TVCVC
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
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問答及客戶評論
■ 常見問題解答
Q:
We are interested in CAT# CSB-EP018491HU, 0.5mg.
I want to know if we can produce the protein PBS buffer +50% glycerol.
Can we guarantee this or no?
A:
Thanks for your inquiry.
1. We can provide Aseptic Processing and Endotoxin Removal sevices for free, pls remark your requirements when placing the order.
2.Regarding your request about the buffer, we can try to use your desired buffer: PBS buffer +50% glycerol, but we are not sure if the protein will be stable under this buffer.
If it's finally proved to be not stable, we will communicate with you in advance and use our default buffer: Tris-based buffer,50% glycerol.
1. We can provide Aseptic Processing and Endotoxin Removal sevices for free, pls remark your requirements when placing the order.
2.Regarding your request about the buffer, we can try to use your desired buffer: PBS buffer +50% glycerol, but we are not sure if the protein will be stable under this buffer.
If it's finally proved to be not stable, we will communicate with you in advance and use our default buffer: Tris-based buffer,50% glycerol.
靶點詳情
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功能：Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.
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基因功能參考文獻：
- The observations suggest a novel role of ATR kinase in mediating its own signal attenuation via PPM1D recruitment to chromatin as an essential mechanism for restarting the stalled forks, cell cycle re-entry and cellular recovery from replication stress. PMID: 29485113
- NF-kappaB served as a positive transcriptional regulator of WIP1 to activate its expression and affect its function in colorectal cancer cells. PMID: 29367109
- ese results enhance our understanding of the molecular mechanisms that govern nucleoli formation by demonstrating that PPM1D regulates nucleolar formation by regulating NPM phosphorylation status through a novel signalling pathway, PPM1D-CDC25C-CDK1-PLK1 PMID: 27619510
- These data support the notion that Wip1 contributes to the formation of the ERMs in PDR membranes via NF-kappaB signaling. PMID: 28402943
- In an in vitro model of the blood-brain barrier, LPS raised Wip1 expression. Wip1 knockdown increased permeability and decreased transepithelial electrical resistance, protein expression of ZO-1, and occluding. Wip1 silencing augmented TNF-alpha, IL-1beta, IL-12, and IL-6. Sonic hedgehog signaling was activated by Wip1 overexpression and inhibited by silencing. Wip1 may protect the BBB against LPS damage via SHH signal... PMID: 29128669
- Studies suugest Wip1 role in tumorigenesis through regulation of p53 and p38MAPK pathways. PMID: 26883196
- The inhibition of Wip1 might fortify p53-mediated tumor suppression by Mdm2 antagonists. PMID: 27183917
- Wip1 suppressed ovarian cancer cell invasion, migration, epithelial to mesenchymal transition (EMT), and ovarian cancer metastasis in xenograft animal models. PMID: 27121065
- model reproduces the observed cellular phenotypes in experiments: oscillatory (for low DNA damage) regulated by negative feedback loops involving mainly p53 and Mdm2 and apoptotic or senescent (for high DNA damage) regulated by the positive p53/Wip1/miR-16 feedback loop PMID: 28968438
- This suggests an important cross-talk between SHH and WIP1 pathways that accelerates tumorigenesis and supports WIP1 inhibition as a potential treatment strategy for MB. PMID: 27086929
- Wip1 activity and its relevance to cancer as an oncoprotein is reviewed PMID: 28385459
- The authors show that a global spread of ATM activity on chromatin and phosphorylation of ATM targets including KAP1 control Plk1 re-activation. These phosphorylations are rapidly counteracted by the chromatin-bound phosphatase Wip1, allowing cell cycle restart despite persistent ATM activity present at DNA lesions. PMID: 28607002
- Truncating and missense PPM1D mutations are associated with prostate cancer. PMID: 27401275
- this study shows that expression of Wip1 is decreased in patients with active inflammatory bowel disease PMID: 27687530
- Wip1 is frequently overexpressed in nonsmall cell lung cancer, which may serve an essential role in the p38MAPK/p53/p16 signaling pathway. PMID: 27959454
- Study identified 14 individuals with mild to severe Intellectual Disability (ID) and/or developmental delay and de novo truncating PPM1D mutations; all mutations were located in the last or penultimate exon, suggesting escape from nonsense-mediated mRNA decay. PMID: 28343630
- PPM1D gene silencing combined with TMZ eradicates glioma cells through cell apoptosis and cell cycle arrest PMID: 27633132
- High PPM1D expression is associated with breast cancer. PMID: 27485113
- PPM1D in the chromosomal location 17q22-24 might be the most relevant candidate gene with respect to a potential functional implication in meningioma progression. PMID: 26942600
- potential prognostic marker and therapeutic target for hepatocellular carcinoma PMID: 26809466
- PPM1D suppresses pancreatic cancer cell apoptosis via inhibition of the p38 MAPK/p53 pathway through both dephosphorylation of p38 MAPK and downregulation of ASPP2. PMID: 26714478
- squalene inhibits the ATM-dependent signaling pathway following DNA damage through intracellular induction of Wip1 expression. PMID: 26824362
- Chemotherapy exposure and age influence the accumulation of PPM1D mutations in the peripheral blood mononuclear cells of ovarian cancer patients. PMID: 26847329
- Knockdown of Wip1 enhances sensitivity to radiation in HeLa cells by inhibiting cell proliferation and inducing apoptosis through activation of p38 MAPK. PMID: 26351212
- Results showed evidence that Wip1 underwent Cdh1-dependent proteolysis during mitosis and sustained Wip1 activity during mitosis, resulting in mitotic delay at the metaphase to anaphase transition. PMID: 25649870
- Study reveals a mechanism of stem cell aging, in which distinct effects of p53 and mTORC1 pathways on HSC aging are governed by Wip1. PMID: 25879755
- WIP1 silencing reduced expression of MMP-9, VEGF-C, cyclin D1, and c-Myc, as well as migration and invasion of salivary adenoid cystic carcinoma cells. WIP1 expression was positively associated with ACC metastasis and prognosis. PMID: 25797250
- Wip1 may be involved in the biological processes of nasopharyngeal cancer cell proliferation, apoptosis, and migration and invasion. PMID: 25060857
- p21 might contribute to positive regulation of WIP1, resulting in dephosphorylation of the histone H2A variant gammaH2AX. PMID: 26006237
- PPM1D plays an important role in promoting CDDP resistance. PMID: 25154814
- data suggests that WIP1 inhibition represents a novel therapeutic approach to neuroblastoma that could be integrated with current chemotherapeutic approaches PMID: 25658463
- ATM kinase and Wip1 phosphatase were identified as opposing regulators of DAXX-S564 phosphorylation and the role of DAXX phosphorylation and DAXX itself are independent of p53-mediated gene expression. PMID: 25659035
- Wip1 protein was increased in kidney carcinoma. PMID: 24970693
- Results show that downregulation of HIPK2 increases resistance of bladder cancer cell to cisplatin by regulating Wip1. PMID: 24846322
- PPM1D plays an important role in the progression of NSCLC. PMID: 25412952
- High PPM1D expression is associated with lung cancer. PMID: 25123458
- The truncating mutations of PPM1D are present in blood DNA of NSCLC patients. PMID: 25742468
- Results demonstrate increased WIP1 expression in metastatic medulloblastomas, and inferior survival in patients with WIP1 high-expressing medulloblastoma. PMID: 24632620
- WIP1 knockdown inhibited the invasiveness of CNE-2 and 5-8F cells and was associated with the down-regulation of the expression of matrix metallopeptidase 9 PMID: 24801909
- Gain-of-function PPM1D mutations are associated with brainstem gliomas. PMID: 24880341
- High PPM1D expression is associated with prostate cancer. PMID: 24922655
- PP4 and Wip1 are differentially required to counteract the p53-dependent cell cycle arrest in G1 and G2, by antagonizing early or late p53-mediated responses, respectively PMID: 24711418
- Wip1 may be involved in the biological processes of non small cell lung cancer cell proliferation, cell apoptosis, and cell cycle. PMID: 24272082
- PPM1D plays a crucial role in colorectal carcinoma cell proliferation and colony formation. PMID: 23745790
- PPM1D mutations in the mosaic state predispose women to breast and ovarian cancer in the absence of a family history of cancer PMID: 24262437
- Expression of Wip1 in neutrophils was downregulated quickly after neutrophil activation through JNK-microRNA-16 pathway. It was negatively correlated with inflammatory cytokine productions of neutrophils in sepsis patients. PMID: 24395919
- Genetic variants and mutations of PPM1D control the response to DNA damage. PMID: 23907125
- BAX dephosphorylation of Wip1 phosphatase is an important regulator of resistance to anticancer therapy. PMID: 23907458
- High WIP1 expression is associated with colorectal cancer. PMID: 23679302
- Wip1 plays an important role in the regulation of global heterochromatin silencing and thus is critical in maintaining genome integrity PMID: 24135283
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相關疾?。?/div>Intellectual developmental disorder with gastrointestinal difficulties and high pain threshold (IDDGIP); Breast cancer (BC); Ovarian cancer (OC)亞細胞定位：Nucleus. Cytoplasm, cytosol.蛋白家族：PP2C family組織特異性：Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.數據庫鏈接：
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