Recombinant Human Polyribonucleotide 5'-hydroxyl-kinase Clp1 (CLP1)

中文名稱(chēng)：

Recombinant Human Polyribonucleotide 5'-hydroxyl-kinase Clp1(CLP1)
貨號(hào)：

CSB-YP842174HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱(chēng)：

Recombinant Human Polyribonucleotide 5'-hydroxyl-kinase Clp1(CLP1)
貨號(hào)：

CSB-EP842174HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱(chēng)：

Recombinant Human Polyribonucleotide 5'-hydroxyl-kinase Clp1(CLP1)
貨號(hào)：

CSB-EP842174HU-B
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱(chēng)：

Recombinant Human Polyribonucleotide 5'-hydroxyl-kinase Clp1(CLP1)
貨號(hào)：

CSB-BP842174HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱(chēng)：

Recombinant Human Polyribonucleotide 5'-hydroxyl-kinase Clp1(CLP1)
貨號(hào)：

CSB-MP842174HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

CLP1
Uniprot No.：

Q92989
別名：

ATP/GTP binding protein; Cleavage and polyadenylation factor I subunit 1; CLP 1; clp1; CLP1 cleavage and polyadenylation factor I subunit homolog; CLP1; cleavage and polyadenylation factor I subunit; homolog (S. cerevisiae); CLP1; yeast; homolog of; CLP1_HUMAN; hClp1; Homolog of yeast CFIA subunit Clp1p; Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Polyribonucleotide 5' hydroxyl kinase Clp1; Polyribonucleotide 5''-hydroxyl-kinase Clp1; Pre mRNA cleavage complex II protein Clp1; Pre-mRNA cleavage complex II protein Clp1
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-425
氨基酸序列

MGEEANDDKK PTTKFELERE TELRFEVEAS QSVQLELLTG MAEIFGTELT RNKKFTFDAG AKVAVFTWHG CSVQLSGRTE VAYVSKDTPM LLYLNTHTAL EQMRRQAEKE EERGPRVMVV GPTDVGKSTV CRLLLNYAVR LGRRPTYVEL DVGQGSVSIP GTMGALYIER PADVEEGFSI QAPLVYHFGS TTPGTNIKLY NKITSRLADV FNQRCEVNRR ASVSGCVINT CGWVKGSGYQ ALVHAASAFE VDVVVVLDQE RLYNELKRDL PHFVRTVLLP KSGGVVERSK DFRRECRDER IREYFYGFRG CFYPHAFNVK FSDVKIYKVG APTIPDSCLP LGMSQEDNQL KLVPVTPGRD MVHHLLSVST AEGTEENLSE TSVAGFIVVT SVDLEHQVFT VLSPAPRPLP KNFLLIMDIR FMDLK
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
基因功能參考文獻(xiàn)：
1. Study reports the identification of a human CLP1 mutation that impairs tRNA splicing in vitro and causes a neurological syndrome involving both the central nervous system (CNS) and peripheral nervous system (PNS). PMID: 24766809
2. Data link tRNA maturation to neuronal development and neurodegeneration through defective CLP1 function in humans. PMID: 24766810
3. the kinase hClp1 phosphorylates and licenses synthetic siRNAs to become assembled into RISC for subsequent target RNA cleavage PMID: 17495927
4. Expression of hClp1 in budding yeast can complement conditional and lethal mutations in the essential 5'-OH RNA kinase module of yeast or plant tRNA ligases. PMID: 18648070
5. HEAB is a pre-mRNA 3'-end processing factor, which bridges two other pre-mRNA processing factors (CF Im and CPSF) and is involved in cleavage of the pre-mRNA. It can be UV-crosslinked to GTP. PMID: 11060040
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相關(guān)疾病：

Pontocerebellar hypoplasia 10 (PCH10)
亞細(xì)胞定位：

Nucleus.
蛋白家族：

Clp1 family, Clp1 subfamily
數(shù)據(jù)庫(kù)鏈接：

HGNC: 16999

OMIM: 608757

KEGG: hsa:10978

STRING: 9606.ENSP00000434995

UniGene: Hs.523687