Recombinant Human Phosphatidylinositol 4-kinase beta (PI4KB), partial

中文名稱：

人PI4KB重組蛋白
貨號：

CSB-YP883360HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人PI4KB重組蛋白
貨號：

CSB-EP883360HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人PI4KB重組蛋白
貨號：

CSB-EP883360HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人PI4KB重組蛋白
貨號：

CSB-BP883360HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人PI4KB重組蛋白
貨號：

CSB-MP883360HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

PI4KB
Uniprot No.：

Q9UBF8
別名：

DKFZp686O1820; FLJ30129; NPIK; Phosphatidylinositol 4 kinase beta; Phosphatidylinositol 4 kinase catalytic beta; Phosphatidylinositol 4 kinase catalytic beta polypeptide; Phosphatidylinositol 4 kinase wortmannin sensitive; Phosphatidylinositol 4-kinase beta; PI4K BETA; PI4K-beta; PI4K92; PI4KB; PI4KB_HUMAN; PI4Kbeta; PI4KIIIbeta; PIK4CB; PtdIns 4 kinase; PtdIns 4-kinase beta; Type III phosphatidylinositol 4 kinase beta
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking.; (Microbial infection) Plays an essential role in Aichi virus RNA replication. Recruited by ACBD3 at the viral replication sites.; (Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV.
基因功能參考文獻：
1. PI4KIIIbeta interaction with the VHS domain of GGA2 affected PI4KIIIbeta localization. PMID: 28289207
2. several disordered regions of PI4KB become protected from proteolytical degradation upon 14-3-3 binding. PMID: 28864297
3. esults show that Aichi virus 3A protein activates the lipid kinase activity of PI4KIIIb,which activation is sensitized by the protein ACBD3. The interfaces between PI4KIIIbeta-ACBD3 and ACBD3-3A were mapped with hydrogen-deuterium exchange mass spectrometry. PMID: 27989622
4. Data show that ACBD3 can recruit PI4KB to model membranes as well as redirect PI4KB to cellular membranes where it is not naturally found. Also, results show that ACBD3 regulates the enzymatic activity of PI4KB kinase through membrane recruitment rather than allostery. PMID: 27009356
5. Analysis reveals novel aspects of the PI4KIIIb-Rab11 complex and determines binding and catalytic sites of the kinase. PMID: 26756197
6. PI4KIIIbeta likely plays a role in breast oncogenesis and that cooperation between Rab11a and PI4KIIIbeta represents a novel Akt activation pathway. PMID: 24962317
7. Although human rhinovirus 3A protein was previously shown to interact with ACBD3, these data suggest that PI4KIIIbeta recruitment occurred independently of both GBF1 and ACBD3. PMID: 25410869
8. Authors found that NS5A and PI4KB competed for association of acyl-coenzyme A binding domain containing protein 3 (ACBD3), which inhibited hepatitis C virus replication. PMID: 24792752
9. Host ACBD3, PI4KIIIBETA and Aichi virus proteins complexes enhances phosphatidylinositol 4-phosphate synthesis and is critical for viral replication. PMID: 24672044
10. these data provide a mechanism for how PI4KIIIbeta coordinates Rab11 and its effectors on PI4P-enriched membranes and also provide strategies for the design of specific inhibitors that could potentially target plasmodial PI4KIIIbeta to combat malaria. PMID: 24876499
11. The lipid kinase PI4KIIIbeta preserves lysosomal identity. PMID: 23258225
12. During authentic HCV infection, PI4P plays an integral role in virus replication; the vesicular transport proteins ARF1 and GBF1 colocalized with PI4KIIIbeta and were both required for HCV replication. PMID: 22359663
13. The 3A protein of picornaviruses utilizes the golgi adaptor protein ACBD3 to recruit PI4KIIIbeta. PMID: 22258260
14. Phosphatidylinositol 4-kinase IIIbeta is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry. PMID: 22253445
15. results indicate that a viral protein/ACBD3/PI4KB complex is formed to synthesize PI4P at the AiV RNA replication sites and plays an essential role in viral RNA replication PMID: 22124328
16. Our data establish PtdIns(4,5)P(2) as a direct activator of TRPV6 and demonstrate that intracellular ATP regulates the channel indirectly as a substrate for type III PI4Ks PMID: 21810903
17. The PI4KIIIbeta likely plays an important role in mammary neoplasia and acinar development. PMID: 21851817
18. Knockdown of PI4KIIalpha does not inhibit EGF-stimulated Akt phosphorylation. PMID: 21218173
19. Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments PMID: 11923287
20. determination that the enzyme is primarily cytosolic, and it associates peripherally with plasma membranes, endoplasmic reticulum, and the Golgi PMID: 12324459
21. PI 4-kinase beta is a key enzyme in the control of spingomyelin synthesis by controlling the flow of ceramide from the ER to the Golgi compartment PMID: 17003043
22. there is a physical and functional relationship between eEF1A2 and PI4KIIIbeta PMID: 17088255
23. Work suggests an important role for both eEF1A2 and PI4KIIIbeta in the control of PI(4,5)P(2) signaling and actin remodeling. PMID: 18474610
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亞細胞定位：

Endomembrane system. Mitochondrion outer membrane; Peripheral membrane protein. Rough endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus. Golgi apparatus membrane. Cytoplasm, perinuclear region.
蛋白家族：

PI3/PI4-kinase family, Type III PI4K subfamily
組織特異性：

Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
數據庫鏈接：

HGNC: 8984

OMIM: 602758

KEGG: hsa:5298

STRING: 9606.ENSP00000271657

UniGene: Hs.632465