Recombinant Human Nectin-1(NECTIN1), partial, Biotinylated

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中文名稱：

Recombinant Human Nectin-1(NECTIN1), partial, Biotinylated
貨號：

CSB-MP621867HU-B
說明書：

MSDS Datasheet
規格：
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The purity of NECTIN1 was greater than 95% as determined by SEC-HPLC
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
生物活性：

Not Test
基因名：

NECTIN1
Uniprot No.：

Q15223
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

38.5 kDa
表達區域：

31-334aa
氨基酸序列

QVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKSTNGSKQNVAIYNPSMGVSVLAPYRERVEFLRPSFTDGTIRLSRLELEDEGVYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRAKKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHQQSLACIVNYHMDRFKESLTLNVQYEPEVTIEGFDGNWYLQRMDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFKGPINYSLAGTYICEATNPIGTRSGQVEVNIT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 10xHis-Avi-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4. Has some neurite outgrowth-promoting activity.; (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV.
基因功能參考文獻：
1. this is the first report showing an association between nectin1 expression and histological subtypes of gastric cancer PMID: 30221498
2. Pseudorabies virus infects host cells via both human and swine nectin-1. PMID: 28542478
3. In a transgenic mouse line with high expression of nectin-1Ig, significant protection from the infection with 30 and 300 MLD50 was observed . PMID: 28671524
4. nectin-1 is expressed in human hair follicles and there is a p63-responsive element in the NECTIN1 promoter PMID: 27516130
5. The viral entry receptor Nectin-1 is also internalized during HSV-1 infection in a Cbl-dependent mechanism, and that increases the opportunity of the virus to spread to uninfected cells. PMID: 28381567
6. HSV gD is able to disrupt intercellular homophilic trans-interaction of nectin-1 and induce a rapid redistribution of nectin-1 from cell junctions. PMID: 27723487
7. PVRL1 variants make a contribution to non syndromic Cleft Lip with/without Cleft palate in Turkish patients. PMID: 26953873
8. PDZD11 forms a complex with nectin-1 and nectin-3, and its PDZ domain interacts directly with the PDZ-binding motif of nectin-1. PMID: 27044745
9. mutations in exons 2 and 5 of PVRL1, and T334A, A391T, G1183A in the alpha-spliced transcript, and G1082T in the beta-spliced transcript do not participate in the development of non-syndromic cleft of the lip and/or palate in patients from Guangdong. PMID: 25966106
10. data suggests that E-cadherin regulates assembly of nectin junctions through alpha-catenin-induced remodeling of the actin cytoskeleton around the cadherin clusters. PMID: 25395582
11. The results showed that the interface between the BV gD and nectin-1 molecule is not geometrically complementary. PMID: 24902525
12. These data provided solid structural and functional evidence that herpes simplex virus 1 and herpes simplex virus 2 gD proteins recognize nectin-1 via the same binding mode. PMID: 25231300
13. Authors show that the same key residues in the BC and FG loops of nectin-4 govern binding to the measles virus attachment protein hemagglutinin (H) and cell entry, nectin-4 homodimerization, and heterodimerization with nectin-1. PMID: 25275122
14. Host nectin-1 is required for efficient Chlamydia trachomatis serovar E development. PMID: 25414835
15. These results suggest that HVEM might play more important roles than CD28 in ConA-mediated T cell proliferation PMID: 24163161
16. We identified new supportive evidence that the association between PVRL1 gene and nsCL/P in Turkish patients. PMID: 24581844
17. Glycoprotein D (gD) epitope core structure maps to the external surface of herpesvirus (HSV) gD, corresponding to the binding sites of two receptors, herpesvirus entry mediator (HVEM) and nectin-1, which mediate HSV infection. PMID: 24100313
18. The conformation of the N-terminus of herpes simplex virus gD is induced by direct binding to HVEM and nectin-1. PMID: 24314649
19. This study demonistated that increased expression of nectin-1 in MS lesions plays a role in the pathogenesis of MS through participation in axonal responses to injury and mediation of altered neuron-glia interactions relevant to myelination. PMID: 22460696
20. Nectin-1 Ig3 induced phosphorylation of FGFR1c in the same manner as the whole nectin-1 ectodomain, and promoted survival of cerebellar granule neurons induced to undergo apoptosis. PMID: 22955284
21. NSCL/P-associated mutations of PVRL1 PMID: 22455396
22. alphaVbeta3-integrin relocalizes nectin1 to lipid rafts, independently of herpes simplex virus 1. PMID: 22171266
23. Structure of herpes simplex virus glycoprotein D bound to the human receptor nectin-1. PMID: 21980294
24. A nonpredicted link between SNAI1-triggered EMT and the down-regulation of Nectin-1 and StarD10 through the up-regulation of miR-661, which may contribute to the invasion of breast cancer cells and poor disease outcome. PMID: 20543867
25. alpha- and gamma-secretase processing of nectin-1 is a Ca(2+)/calmodulin-regulated event that occurs under conditions of activity-dependent synaptic plasticity and ADAM10 and gamma-secretase are responsible for these cleavage events. PMID: 20501653
26. There is no relationship between nonsyndromic cleft lip and/or palate and PVRL1exon3 in Han People. PMID: 19408722
27. results suggest that PVRL1 may play a minor role in susceptibility to the occurrence of nsCL/P in some Caucasian populations, and that variation involving the beta (HIgR) isoform might have particular importance for risk of orofacial clefts PMID: 19715471
28. evidence that nectin-1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry receptor PMID: 12072519
29. association of HVEM and nectin-1 with lipid rafts during herpes simplex virus entry PMID: 12915568
30. both nectin 1 and HVEM receptors play a role during HSV infection in vivo and both are highly efficient even at low levels of expression PMID: 15110526
31. Nectin-1-Delta2 exhibited a severely reduced ability to mediate HSV entry and accumulated in the endoplasmic reticulum but retained the ability to interact with its HSV ligand, gD. PMID: 15731277
32. trans-interacting nectin inhibits non-trans-interacting E-cadherin endocytosis through afadin, Rap1, and p120ctn and further accumulates non-trans-interacting E-cadherin to the nectin-based cell-cell adhesion sites for the formation of adherens junctions PMID: 15857834
33. mutations at codons 185 and 323, especially the W185X mutation, do not participate in the formation of cleft lip and palate within the Taiwanese population examined PMID: 16497481
34. Mutations within the PVRL1 gene represent risk factors for non-syndromic cleft lip with or without cleft palate. PMID: 16674562
35. Access to nectin-1 contributes to preferential apical infection of human epithelial cells by herpes simplex virus. PMID: 17005657
36. the role of PVRL1 in the sporadic forms of orofacial clefting in multiple populations was studied. PMID: 17089422
37. nectin-1 may be used as a marker to predict the sensitivity of a tumor to herpes oncolytic therapy PMID: 17164781
38. These data suggest that the determinants of gD-mediated internalization of nectin-1 may direct HSV to an endocytic pathway during entry. PMID: 18076965
39. Novel non-synonymous PVRL1 mutation of the substitution of valine by methionine a position 395 in a conserved sequence suggests a possible etiologic role of PVRL1 in non-syndromic CL/P across different populations. PMID: 18356023
40. Western blot analyses demonstrated that accumulation of host nectin-1 is decreased by 85 % at 48 hours post-infection (h.p.i.) in Chlamydia trachomatis serovar E-infected HeLa cells. PMID: 18451037
41. Antibodies against nectin-1, but not HVEM, were able to block HSV-1 infection.Anti-nectin-1 antibodies and F-actin depolymerizers were also successful in blocking the cytoskeletal changes that occur upon HSV-1 entry into cells PMID: 18803666
42. These results indicate that nectin-1 is degraded by chlamydial protease-like activity factor (CPAF) in Chlamydia trachomatis-infected genital epithelial cells, a novel strategy that Chlamydiae may use to aid their dissemination. PMID: 18983929
43. Insertional mutations of gB reduced cell fusion activity when PILRalpha was overexpressed much more than nectin-1. PMID: 19457990
44. Regions of nectin-1 protein important for herpesvirus entry activity and homotypic nectin-nectin interactions are overlapping but not identical. PMID: 12438620
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相關疾病：

Ectodermal dysplasia, Margarita Island type (EDMI); Non-syndromic orofacial cleft 7 (OFC7)
亞細胞定位：

[Isoform Alpha]: Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, presynaptic cell membrane.; [Isoform Delta]: Cell membrane; Single-pass type I membrane protein.; [Isoform Gamma]: Secreted.
蛋白家族：

Nectin family
數據庫鏈接：

HGNC: 9706

OMIM: 225060

KEGG: hsa:5818

STRING: 9606.ENSP00000264025

UniGene: Hs.334846