Recombinant Human Na (+)/H (+) exchange regulatory cofactor NHE-RF2 (SLC9A3R2)

中文名稱：

人SLC9A3R2重組蛋白
貨號：

CSB-YP620885HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人SLC9A3R2重組蛋白
貨號：

CSB-EP620885HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人SLC9A3R2重組蛋白
貨號：

CSB-EP620885HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人SLC9A3R2重組蛋白
貨號：

CSB-BP620885HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人SLC9A3R2重組蛋白
貨號：

CSB-MP620885HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

SLC9A3R2
Uniprot No.：

Q15599
別名：

E3KARP; HGNC:11076; Isoform 3 regulator 2; Isoform 3 regulatory factor 2; MGC104639; Na(+)/H(+) exchange regulatory cofactor NHE RF2; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 kinase A regulatory protein; NHE3 kinase A regulatory protein E3KARP; NHE3 regulatory factor 2; NHE3RF2; NHERF 2; NHERF-2; NHERF2; NHRF2_HUMAN; OCTS2; SIP 1; SIP-1; SIP1; Slc9a3r2; Sodium hydrogen exchanger regulatory factor 2; Sodium-hydrogen exchanger regulatory factor 2; Sodium/hydrogen exchanger; Sodium/hydrogen exchanger regulatory factor 2; Solute carrier family 9 (sodium/hydrogen exchanger); Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulator 2; Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulatory factor 2; Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 2; Solute carrier family 9 isoform 3 regulator 2; Solute carrier family 9 isoform A3 regulatory factor 2; SRY interacting protein 1; SRY-interacting protein 1; TKA 1; TKA-1; TKA1; Tyrosine kinase activator protein 1
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-337
氨基酸序列

MAAPEPLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTRLLVVDQ ETDEELRRRQ LTCTEEMAQR GLPPAHDPWE PKPDWAHTGS HSSEAGKKDV SGPLRELRPR LCHLRKGPQG YGFNLHSDKS RPGQYIRSVD PGSPAARSGL RAQDRLIEVN GQNVEGLRHA EVVASIKARE DEARLLVVDP ETDEHFKRLR VTPTEEHVEG PLPSPVTNGT SPAQLNGGSA CSSRSDLPGS DKDTEDGSAW KQDPFQESGL HLSPTAAEAK EKARAMRVNK RAPQMDWNRK REIFSNF
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.
基因功能參考文獻(xiàn)：
1. Studies support a role for NHERF-1 and NHERF-2 (Na+/H+ exchanger regulatory factors 1 and 2) in regulating the distribution of Group II metabotropic glutamate receptor (mGluRs) in the murine brain, while conversely the effects of the mGluR2/3 PDZ-binding motifs on receptor signaling are likely mediated by interactions with other PDZ scaffold proteins beyond the NHERF proteins. PMID: 28392297
2. Moreover, the S303D mutation enhances the in vivo dynamics of the E3KARP tail alone, whereas in vitro the interaction of E3KARP with active ezrin is unaffected by S303D PMID: 26310448
3. Lysophosphatidic acid stimulation of NHE3 exocytosis in polarized epithelial cells occurs with release from NHERF2 via ERK-PLC-PKCdelta signaling. PMID: 24760985
4. Data indicate that the tails promote different microvillar localizations for EBP50 and E3KARP, which localized along the full length and to the base of microvilli, respectively. PMID: 23985317
5. NHERF2 domain was functionally significant in NHE3 regulation, being necessary for stimulation by lysophosphatidic acid of activity and increased mobility of NHE3 PMID: 23612977
6. functional regulation of C3aR by NHERFs in human mast cells PMID: 23284683
7. AnxA2 and NHERF2 form a scaffold complex that links adjacent Tir molecules at the plasma membrane forming a lattice that could be involved in retention and dissemination of other effectors at the bacterial attachment site. PMID: 22587461
8. NHERF-2 is a negative regulator of endothelial proliferation and may have important roles in endothelial homeostasis and vascular modeling. PMID: 22343917
9. CaMKII inhibition of NHE3 reguires NHEF2. PMID: 22371496
10. Data show that both NHERF2 and NHERF1 are involved in setting NHE3 activity. PMID: 21191106
11. The results of this study revealed that NHERF-2 can interact with GLAST in astrocytes to enhance GLAST stability and activity. PMID: 20430067
12. WNK4 and NHERF2 synergistically regulate TRPV5 by enhancing its forward trafficking and increasing its stability at plasma membrane, respectively. PMID: 21187068
13. The authors generated a HeLa cell line stably expressing HA-tagged NHERF2 and found that Map, EspI and NleH1 colocalize and interact with intracellular NHERF2 via their C-terminal PDZ-binding motif. PMID: 20618342
14. Apical scaffolding protein NHERF2 modulates the localization of alternatively spliced plasma membrane Ca2+ pump 2B variants in polarized epithelial cells. PMID: 20663896
15. NHERF1 and NHERF2 exhibit isotype-specific effects on G protein activation. PMID: 20562104
16. Plasma membrane Ca2+ ATPase isoform 2b interacts preferentially with Na+/H+ exchanger regulatory factor 2 in apical plasma membranes PMID: 11786550
17. E3KARP has a restricted tissue distribution with the highest expression being found in lung. It is largely colocalized with moesin and radixin, especially in the alveoli of the lung, as well as being highly enriched in the renal corpuscle. PMID: 11893083
18. Na(+)/H(+ ) exchanger regulatory factor 2 directs parathyroid hormone 1 receptor signalling. PMID: 12075354
19. A2BR binds to E3KARP upon agonist stimulation. PMID: 12080047
20. When the second PDZ domain of E3KARP is bound to down regulated in adenoma (dra) gene product, a structural link is built between the functionally coupled Na+/H+ and Cl-/HCO3- exchangers in the proximal colon. PMID: 12369822
21. NHERF2 and SGK1 interact to enhance ROMK1 activity by enhancing abundance of channel protein in cell membrane, allowing integration of genomic regulation and activation of SGK1 and NHERF2 in control of ROMK1 activity and renal K(+) excretion. PMID: 12444200
22. NHERF2, with ROMK1 and SGK1 has a role in regulating protein abundance in the plasma membrane and K(+) current PMID: 14623317
23. demonstration of the significance of SGK1 and NHERF2 as TRPV5 modulators which are likely to participate in the regulation of calcium homeostasis by 1,25(OH)2D3 PMID: 15665527
24. the function of SIP-1/NHERF2 as an SRY cofactor during testis determination is conserved between human and mouse PMID: 16166090
25. analysis of NHERF recognition by ERM proteins PMID: 16615918
26. N-cadherin and beta-catenin play role in cell migration via PDGF-Rbeta-mediated signaling through the scaffolding molecule NHERF2 PMID: 17229887
27. While the presence of forskolin results in an increase in OCTN2 protein expression, the increase in uptake capacity may be compensated by the decreased expression of PDZK1, NHERF1 or NHERF2. PMID: 19091402
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亞細(xì)胞定位：

Endomembrane system; Peripheral membrane protein. Nucleus. Apical cell membrane.
組織特異性：

Widely expressed.
數(shù)據(jù)庫鏈接：

HGNC: 11076

OMIM: 606553

KEGG: hsa:9351

STRING: 9606.ENSP00000408005

UniGene: Hs.440896