Recombinant Human Msx2-interacting protein (SPEN), partial

中文名稱：

人SPEN重組蛋白
貨號：

CSB-YP842763HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人SPEN重組蛋白
貨號：

CSB-EP842763HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人SPEN重組蛋白
貨號：

CSB-EP842763HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人SPEN重組蛋白
貨號：

CSB-BP842763HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人SPEN重組蛋白
貨號：

CSB-MP842763HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SPEN
Uniprot No.：

Q96T58
別名：

SPEN; KIAA0929; MINT; SHARP; Msx2-interacting protein; SMART/HDAC1-associated repressor protein; SPEN homolog
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.
基因功能參考文獻：
1. our data demonstrate a role for SPEN in the regulation of primary cilia formation and cell migration in breast cancer cells, which may collectively explain why its expression is associated with time to metastasis in cohorts of patients with ERalpha-negative breast cancers. PMID: 28877752
2. SPEN is a novel tumor-suppressor gene that may be clinically useful as a predictive biomarker of tamoxifen response in ERalpha-positive breast cancers. PMID: 26297734
3. SPEN mutations were associated with diffuse large B-cell lymphoma with hepatitis C virus infection. PMID: 25381127
4. Phosphorylation of the CK2 site on SMRT significantly increased affinity for SHARP. PMID: 24268649
5. A structural study of the RNA binding properties of the RNA recognition motifs of SHARP. PMID: 24748666
6. These results demonstrate a role for the inactivation of SHARP transcription in DM1 biology. PMID: 21637295
7. MINT3 and MINT17 were informative for patient grouping to predict local recurrence in rectal cancer patients PMID: 20460484
8. SHARP is a novel component of the HDAC corepressor complex, recruited by RBP-Jkappa to repress transcription of target genes in the absence of activated Notch. PMID: 12374742
9. the conserved function of the SPOC domain of SHARP is to mediate interaction with SMRT/NCoR corepressors, and that Spen proteins play an essential role in the repression complex PMID: 12897056
10. both the androgen receptor interacting domains of the coactivator SRC1 and of the corepressor SMRT compete for interaction with the androgen receptor N-terminus PMID: 15062576
11. Pak1-SHARP interaction plays an essential role in enhancing the corepressor functions of SHARP, thereby modulating Notch signaling in human cancer cells. PMID: 15824732
12. Msx2-interacting protein(MINT), human interacts with the UbcH8. PMID: 16583136
13. SHARP gene and protein expression is elevated in human colon and ovarian endometrioid adenocarcinomas carrying gene defects leading to beta-catenin dysregulation. PMID: 17234755
14. In functional assays, corepressor ETO, but not AML1/ETO, augments SHARP-mediated repression in an histone deacetylase-dependent manner. PMID: 18332109
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亞細胞定位：

Nucleus. Note=Associates with chromatin.
蛋白家族：

RRM Spen family
組織特異性：

Expressed at high level in brain, testis, spleen and thymus. Expressed at intermediate level in kidney, liver, mammary gland and skin.
數據庫鏈接：

HGNC: 17575

OMIM: 613484

KEGG: hsa:23013

STRING: 9606.ENSP00000364912

UniGene: Hs.744843