Recombinant Human Lysine--tRNA ligase (KARS)

中文名稱：

人KARS重組蛋白
貨號：

CSB-YP618973HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人KARS重組蛋白
貨號：

CSB-EP618973HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人KARS重組蛋白
貨號：

CSB-EP618973HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人KARS重組蛋白
貨號：

CSB-BP618973HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人KARS重組蛋白
貨號：

CSB-MP618973HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

KARS
Uniprot No.：

Q15046
別名：

CMTRIB; DFNB89; EC 6.1.1.6; KARS 1; KARS 2; KARS; KARS1; KARS2; KIAA0070; KRS; Lysine tRNA ligase; Lysine--tRNA ligase; LysRS; Lysyl tRNA synthetase; Lysyl-tRNA synthetase; SYK_HUMAN
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-597
氨基酸序列

AAVQAAEVK VDGSEPKLSK NELKRRLKAE KKVAEKEAKQ KELSEKQLSQ ATAAATNHTT DNGVGPEEES VDPNQYYKIR SQAIHQLKVN GEDPYPHKFH VDISLTDFIQ KYSHLQPGDH LTDITLKVAG RIHAKRASGG KLIFYDLRGE GVKLQVMANS RNYKSEEEFI HINNKLRRGD IIGVQGNPGK TKKGELSIIP YEITLLSPCL HMLPHLHFGL KDKETRYRQR YLDLILNDFV RQKFIIRSKI ITYIRSFLDE LGFLEIETPM MNIIPGGAVA KPFITYHNEL DMNLYMRIAP ELYHKMLVVG GIDRVYEIGR QFRNEGIDLT HNPEFTTCEF YMAYADYHDL MEITEKMVSG MVKHITGSYK VTYHPDGPEG QAYDVDFTPP FRRINMVEEL EKALGMKLPE TNLFETEETR KILDDICVAK AVECPPPRTT ARLLDKLVGE FLEVTCINPT FICDHPQIMS PLAKWHRSKE GLTERFELFV MKKEICNAYT ELNDPMRQRQ LFEEQAKAKA AGDDEAMFID ENFCTALEYG LPPTAGWGMG IDRVAMFLTD SNNIKEVLLF PAMKPEDKKE NVATTDTLES TTVGTSV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

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Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.; (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
基因功能參考文獻：
1. The studies showed that mutations in KARS lead to a newly defined subtype of leukoencephalopathy associated with sensorineural hearing impairment. The combined effect of reduced aminoacylation and release of LysRS from the multiple-synthetase complex (MSC) likely underlies the pathogenesis of the KARS mutations identified in this study. PMID: 28887846
2. Using the TruSight One sequencing panel we identified two novel mutations in KARS. Both mutations, never reported previously, occur in a highly conserved region of the catalytic domain and displayed a dramatic effect on KARS stability PMID: 27891585
3. Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from colorectal cancer cells. PMID: 28611052
4. Results indicate that KRS can promote cell-cell and cellextracellular matrix adhesion for migration. PMID: 26891990
5. finding show that enzymatically active Shiga toxins trigger the dissociation of lysyl-tRNA synthetase (KRS) from the multi-aminoacyl-tRNA synthetase complex in human macrophage-like differentiated THP-1 cells and its subsequent secretion. PMID: 26643967
6. KRS at the plasma membrane plays new roles in metastatic migration as a signaling inducer, and causes intracellular signaling for cancer dissemination PMID: 26091349
7. tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939
8. structural characteristics of the KRS-LR interaction on the cell surface PMID: 24983501
9. Lysyl-tRNA synthetase plays essential role in HIV replication, transcriptional regulation, cytokine-like signaling. [review] PMID: 23972532
10. The role of preKARS2 in the tRNA mitochondrial import. PMID: 23799079
11. The KARS variant is identified in two families affected by DFNB89-associated autosomal-recessive nonsyndromic hearing impairment. PMID: 23768514
12. C-terminal domain of HIV-1 capsid protein as surrogate for human lysyl tRNA synthetase PMID: 23208549
13. A single conformational change triggered by phosphorylation leads to multiple effects driving an exclusive switch of LysRS function from translation to transcription. PMID: 23159739
14. Dual role for motif 1 residues of human lysyl-tRNA synthetase in dimerization and packaging into HIV-1. PMID: 23095741
15. The work thus unveiled a unique function of KRS in the control of cell migration and its pathological implication in metastasis. PMID: 22751010
16. Data are consistent with hypothesis that maturation of cytoplasmic KARS precursor is needed to reveal the potent tRNA binding properties of mitochondrial KARS. PMID: 22235746
17. LysRS associates with the Pol domain of GagPol. PMID: 21763493
18. The results suggest that this unique geometry, which reconfigures the LysRS tetramer from alpha(2):alpha(2) to alpha(2)beta(1):beta(1)alpha(2), is designed to control both retention and mobilization of LysRS from the multi-tRNA synthetase complex PMID: 21536907
19. The interaction between helix 7 of LysRS and helix 4 of the capsid C-terminal domain of HIV-1 Gag (HIV-CA-CTD) was studied using circular dichromism spectroscopy and molecular dynamics simulation. PMID: 21093454
20. These results underscore the contribution of KARS to the emission of (one of) the principal signal(s) of immunogenic cell death, CRT exposure. PMID: 20699648
21. Loss-of-function lysyl-tRNA synthetase mutations associated with peripheral neuropathy and Charcot-Marie-Tooth disease. PMID: 20920668
22. HIV-1 Gag interacts with human lysyl-tRNA synthetase during viral assembly PMID: 12756246
23. packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag; this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1 PMID: 15220430
24. analysis of the interaction between HIV-1 Gag and human lysyl-tRNA synthetase PMID: 16702215
25. HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial Lysyl-tRNA synthase. PMID: 17560997
26. This work reports further characterization of the interaction between HIV-1 capsid domain of Gag and human LysRS using truncation constructs and point mutations in the putative interaction helices. PMID: 17724017
27. present a 2.3-A crystal structure of a tetrameric form of human LysRS PMID: 18272479
28. mitoKARS is the first described member of a group of mitochondrial proteins whose interaction with mutant SOD1 contributes to mitochondrial dysfunction in ALS PMID: 18715867
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相關疾病：

Charcot-Marie-Tooth disease, recessive, intermediate type, B (CMTRIB); Deafness, autosomal recessive, 89 (DFNB89)
亞細胞定位：

[Isoform Cytoplasmic]: Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted.; [Isoform Mitochondrial]: Mitochondrion.
蛋白家族：

Class-II aminoacyl-tRNA synthetase family
數據庫鏈接：

HGNC: 6215

OMIM: 601421

KEGG: hsa:3735

STRING: 9606.ENSP00000325448

UniGene: Hs.3100