Recombinant Human Low-density lipoprotein receptor-related protein 2 (LRP2), partial
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中文名稱：人LRP2重組蛋白
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貨號：CSB-YP013096HUc7
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說明書：
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規格：￥1500
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圖片：
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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其他：
產品詳情
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純度：Greater than 90% as determined by SDS-PAGE.
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基因名：
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Uniprot No.：
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別名：(LRP-2)(Glycoprotein 330)(gp330)(Megalin)
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種屬：Homo sapiens (Human)
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蛋白長度：Partial
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來源：Yeast
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分子量：23.7 kDa
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表達區域：1186-1389aa
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氨基酸序列NCTASQFKCASGDKCIGVTNRCDGVFDCSDNSDEAGCPTRPPGMCHSDEFQCQEDGICIPNFWECDGHPDCLYGSDEHNACVPKTCPSSYFHCDNGNCIHRAWLCDRDNDCGDMSDEKDCPTQPFRCPSWQWQCLGHNICVNLSVVCDGIFDCPNGTDESPLCNGNSCSDFNGGCTHECVQEPFGAKCLCPLGFLLANDSKTCE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering. -
蛋白標簽：C-terminal 6xHis-tagged
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產品提供形式：Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
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復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet & COA：Please contact us to get it.
產品評價
相關產品
靶點詳情
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功能：Multiligand endocytic receptor. Acts together with CUBN to mediate endocytosis of high-density lipoproteins. Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B. In the kidney, mediates the tubular uptake and clearance of leptin. Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium. Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight. Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells. Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP. Mediates renal uptake of metallothionein-bound heavy metals. Together with CUBN, mediates renal reabsorption of myoglobin. Mediates renal uptake and subsequent lysosomal degradation of APOM. Plays a role in kidney selenium homeostasis by mediating renal endocytosis of selenoprotein SEPP1. Mediates renal uptake of the antiapoptotic protein BIRC5/survivin which may be important for functional integrity of the kidney. Mediates renal uptake of matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1. Mediates endocytosis of Sonic hedgehog protein N-product (ShhN), the active product of SHH. Also mediates ShhN transcytosis. In the embryonic neuroepithelium, mediates endocytic uptake and degradation of BMP4, is required for correct SHH localization in the ventral neural tube and plays a role in patterning of the ventral telencephalon. Required at the onset of neurulation to sequester SHH on the apical surface of neuroepithelial cells of the rostral diencephalon ventral midline and to control PTCH1-dependent uptake and intracellular trafficking of SHH. During neurulation, required in neuroepithelial cells for uptake of folate bound to the folate receptor FOLR1 which is necessary for neural tube closure. In the adult brain, negatively regulates BMP signaling in the subependymal zone which enables neurogenesis to proceed. In astrocytes, mediates endocytosis of ALB which is required for the synthesis of the neurotrophic factor oleic acid. Involved in neurite branching. During optic nerve development, required for SHH-mediated migration and proliferation of oligodendrocyte precursor cells. Mediates endocytic uptake and clearance of SHH in the retinal margin which protects retinal progenitor cells from mitogenic stimuli and keeps them quiescent. Plays a role in reproductive organ development by mediating uptake in reproductive tissues of androgen and estrogen bound to the sex hormone binding protein SHBG. Mediates endocytosis of angiotensin-2. Also mediates endocytosis of angiotensis 1-7. Binds to the complex composed of beta-amyloid protein 40 and CLU/APOJ and mediates its endocytosis and lysosomal degradation. Required for embryonic heart development. Required for normal hearing, possibly through interaction with estrogen in the inner ear.
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基因功能參考文獻：
- megalin is critical for mitochondrial biology; mitochondrial intracrine signaling is a continuum of the retrograde early endosome-to-Golgi-Rab32 pathway and defects in this pathway may underlie disease processes in many systems. PMID: 29916093
- patients with OCRL-1 mutations or type 1 Dent disease showed abnormally low levels of urinary A-megalin PMID: 27766457
- An Emerging Role for Megalin as a Regulator of Protein Leak in Acute Lung Injury. PMID: 29090957
- Review of LRP2 function. LRP2 functions are crucial for developmental processes in a number of tissues, including the brain, the eye, and the heart, and defects in this receptor pathway are the cause of devastating congenital diseases in humans. PMID: 26872844
- A novel LRP2 missense variant rs17848169 (N2632D) was found to be associated with lower risk for T2D-ESRD in this population. PMID: 27197912
- we discovered one novel locus (LRP2; most significant single nucleotide polymorphism rs12988804) that reached genome-wide significance in predicting relapse risk (HR=2.18, p=3.30x10(-8)). PMID: 28739605
- miR-148b directly down-regulates renal megalin expression. PMID: 28331063
- Exocytosis-mediated urinary C-megalin excretion is associated with the development and progression of diabetic nephropathy in T2DM, particularly due to megalin-mediated lysosomal dysfunction in proximal tubules. PMID: 28289043
- The studies suggest that impaired endocytosis of megalin/cubilin ligands, hemoglobin and albumin, rather than heme toxicity, may be the cause of tubular proteinuria in sickle cell disease patients. PMID: 28356267
- VDR and MEGALIN gene variations can alter age-related cognitive trajectories differentially between men and women among African American urban adults, specifically in global mental status and domains of verbal fluency, visual/working memory, and executive function. PMID: 28446629
- the main role for placental megalin is not to mediate uptake of nutrients from the maternal bloodstream; results point toward novel and complex functions for megalin in the cytotrophoblasts. PMID: 27798286
- specific miRNA-146a regulation may contribute to Alzheimer's disease by downregulating the Lrp2/Akt pathway PMID: 27241555
- abundances of megalin and Dab2 (p = 0.046) were reduced in infected placentas from women with LBW deliveries PMID: 27072056
- homozygous Asp3779Asn and a hemizygous Ile262Met mutations in the LRP2 and TSPYL2 genes, respectively, in a Pakistani family with two boys affected with mild nonsyndromic intellectual disability PMID: 26529358
- The megalin expression appears to vary inversely with gestational age with the greatest expression noted in the most premature samples. Age-dependent differences in placental megalin may therefore influence fetal exposure. PMID: 25304941
- we are the first to identify the association between LRP2 and gout in a Chinese population and to confirm this association in Asians. PMID: 26147675
- Two novel LRP2 mutations, a homozygous nonsense mutation and a missense mutation in two unrealted families with Donnai-Barrow syndrome. PMID: 25682901
- Loss of LRP2 is associated with buphthalmos PMID: 26439398
- melanoma cell expression of LRP2/megalin significantly decreases melanoma cell proliferation and survival rates. PMID: 25585665
- levels of urinary C-megalin are associated with histological abnormalities in adult IgAN patients PMID: 25502002
- A new mutation in LRP2 causes a predominantly ocular phenotype suggestive of Stickler syndrome. PMID: 23992033
- LRP2 sequencing reveals multiple rare variants associated with urinary trefoil factor-3 PMID: 24876117
- Serum uric acid-related gene LRP2 is not involved in gout susceptibility. PMID: 24366390
- Transgenic/knock-out megalin-deficient mice develop anxiety behavior and impaired learning, as described in Alzheimer's disease. PMID: 24254699
- Association of the T-allele of a single nucleotide polymorphism in LRP2 with gout risk in the Maori and Pacific subjects was consistent with this allele increasing serum urate in Japanese individuals PMID: 24286387
- LOS treatment decreased microalbuminuria induced by Cd apparently through a cubilin receptor-dependent mechanism but independent of megalin. PMID: 24093454
- Data suggest that endodermal layer of yolk sac and syncytiotrophoblast/cytotrophoblast cells of placental villi express megalin mRNA/protein; expression of megalin protein (but not mRNA) is up-regulated as gestation/placentation progresses. PMID: 23978537
- This review explores current evidence linking megalin expression and function to the development, diagnosis, and progression of acute kidney injury --{REVIEW} PMID: 24197071
- Megalin and Dab2 were expressed in prostate and colon epithelial cells, which was markedly enhanced following treatment with retinoic acid PMID: 23909735
- The hypothalamic clusterin-low-density lipoprotein receptor-related protein-2 axis is a novel anorexigenic signalling pathway. PMID: 23673647
- results suggested that GSTT1 wild genotype and C-allele of megalin gene rs2228171 SNPs might be risk factors for cisplatin-induced ototoxicity PMID: 23274376
- Using NMR titration data in HADDOCK, we have generated a three-dimensional model describing the complex between megalin and gentamicin. PMID: 23275343
- Expression of megalin and cubilin is decreased during experimental endotoxemia, which may contribute to an increase in urine levels of albumin during acute renal failure. PMID: 22437417
- analysis of rare disease variants in LRP2, a gene linked and associated with autism spectrum disorders PMID: 22578327
- This study confirmed that LRP2 rs2544390 C/T at intron 1 was associated with serum uric acid levels among Japanese males with SLC22A12 258WW, SLC2A9 rs11722228C allele, ABCG2 126QQ and 141Q allele. PMID: 22565184
- A total of 330 Chinese female-offspring nuclear families with 1088 individuals and 400 Chinese male-offspring nuclear families with 1215 individuals were genotyped at six tag single nucleotide polymorphisms of the LRP2 gene. PMID: 22174918
- Sex-specific VDR and Megalin gene variations can modify age-related cognitive decline among US adults. PMID: 22170372
- megalin and cubilin are involved in the metabolism of vitamin D by reabsorbing vitamin D binding protein; dysfunction of these receptors is likely to be associated with the development of vitamin D deficiency in patients with chronic kidney disease PMID: 21595846
- allele (A) of the rs3755166 polymorphism within LRP2 gene may contribute to Alzheimer's disease risk in the Chinese Han Population PMID: 20971101
- in anagen VI hair follicles megalin was found in all keratinocytes of the distal region PMID: 21104416
- Data show that MT-I + II and megalin are significantly altered in CNS lymphoma relative to controls. PMID: 20038220
- No defect in the trafficking or function of megalin upon OCRL1 knockdown. PMID: 19940034
- Megalin and cubilin: multifunctional endocytic receptors. A review. PMID: 11994745
- This study reveals that LRP2 is a major autoantigen in rheumatoid arthritis and probably drives the production of anti-LRP2 autoantibodies, which may play pathological roles by inhibiting the reabsorbing function of LRP2. PMID: 12723989
- megalin has a role in thyroid homeostasis with possible implications in thyroid diseases PMID: 14657389
- a binding affinity of disabled homolog 2 mitogen-responsive phosphoprotein interaction domain for megalin CT of K(D) = 2.6 x 10(-7) +/- 5.3 x 10(-8) PMID: 15134832
- megalin endocytosed NGAL by a mechanism completely blocked by an antibody against megalin PMID: 15670845
- Further studies on the intracellular molecular signalling associated with megalin-mediated metabolic pathways may lead to the development of novel strategies for the treatment of nephropathies related to diabetes and metabolic syndrome. (Review) PMID: 16174284
- This review focuses on the involvement of megalin during embryonic development and its interactions with the developmental morphogen sonic hedgehog. PMID: 16828734
- Results show that the PPPSP motif and GSK3 activity are critical to allow megalin phosphorylation and also negatively regulate the receptor's recycling. PMID: 17555532
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相關疾病：Donnai-Barrow syndrome (DBS)
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亞細胞定位：Apical cell membrane; Single-pass type I membrane protein. Endosome lumen. Membrane, coated pit. Cell projection, dendrite. Cell projection, axon.
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蛋白家族：LDLR family
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組織特異性：Expressed in first and third trimester cytotrophoblasts in the placenta (at protein level). Absorptive epithelia, including renal proximal tubules.
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