E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized
powder
Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1. Required for the establishment of the specialized cells of the pituitary gland and the nervous system. Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1.
基因功能參考文獻:
Study did not identify HESX1 and LHX3 mutations by Sanger in brazilian patients with combined pituitary hormone deficiency PMID: 28734020
Further functional studies show that forced expression of LHX3 in lung cancer cells obviously promotes cell proliferation and invasion, whereas inhibits cell apoptosis. In summary, LHX3 is an early-stage and radiosensitivity prognostic biomarker, and a novel potential oncogene in lung adenocarcinoma . PMID: 28731174
investigated the specific mutations in PROP1, POU1F1, LHX3, and HESX1 genes in patients with combined pituitary hormone deficiency (CPHD) in Turkey PMID: 25500790
LHX3 is upregulated in high-grade oligodendroglioma. PMID: 25399296
The downstream enhancer region of LHX3 in regulating gene expression at the cellular level during development, is reported. PMID: 24100213
Data suggest that low serum FSH (follicle stimulating hormone) levels in men with SNP in promoter region of FSHB (FSH beta subunit; -211G/T) result from reduced LHX3 binding to FSHB promoter and down-regulation of FSHB transcription in gonadotrophs. PMID: 23766128
The present study was unable to confirm a significant association of all of the three SNPs, rs12338076 in LHX3-QSOX2, and rs1457595 and rs17032362 in IGF1, with adult height in our study population. PMID: 22503243
descriprion of pediatric patients with combined pituitary hormone deficiency with a novel mutation in LHX3; the T194R mutation affects a critical residue in the LHX3 protein; study extends understanding of phenotypic features, molecular mechanism and developmental course associated with mutations in the LHX3 gene PMID: 22286346
This study of the first nonconsanguineous patient with LHX3 mutations demonstrates the pleiotropic roles of LHX3 during development and its full involvement in the complex disease phenotype of syndromic combined pituitary hormone deficiency. PMID: 22238406
This study establishes ISL1 as a novel transcriptional regulator of LHX3 and describes a potential mechanism for regulation by PITX1. PMID: 22194342
LHX3 mutations are a rare cause of hypopituitarism. We report on a patient with a novel LHX3 mutation in exon 2 with the phenotype of combined pituitary hormone deficiency with short neck and sensorineural hearing impairment. [review] PMID: 21249393
A genome-wide association study in 19 633 Japanese subjects identified LHX3-QSOX2 and IGF1 as adult height loci. PMID: 20189936
Mutations in LHX3 are associated with a short neck combination with GHD (growth hormone deficiecy), PRL(prolactin) and TSH (thyroid stimulating hormone) and gonadotropin deficiency p. 278 PMID: 14646405
Mutations within LHX3 are associated with recessive combined pituitary hormone deficiency, with sparing of cortisol secretion... P. 207 PMID: 14714741
LHX3 LIM homeodomain transcription factor is involved in activation of the FSH beta-subunit gene in the pituitary gonadotrope cell. PMID: 15271874
identified unique amino acids within LHX3 that are important for its transcriptional activity and are phosphorylated PMID: 15517599
LHX3 is expressed at all stages of early development. PMID: 15567726
Specificity protein 1 is a regulator of both promoters through interaction with GC boxes and also, a distal element within intron 1a that is recognized by nuclear factor I is critical for hLHX3b promoter function. PMID: 16179410
The presence of a hypointense pituitary lesion and other clinical findings broadens the phenotype associated with LHX3 gene mutation. PMID: 16394081
Lhx3expression of Isl-1 and Lhx3, together with steroidogenic factor 1 (SF-1), culminates in the activation of both the rat as well as human GnRH-R promoter, suggesting that this combination is evolutionarily conserved among mammals PMID: 16613990
in 7 combined pituitary hormone deficiency patients from 4 consanguineous pedigrees, 4 novel, recessive mutations were identified: a deletion of the entire gene, mutations causing truncated proteins & a mutation causing a substitution in the homeodomain PMID: 17327381
LHX3 mutations is associated with sensorineural hearing loss and interaction between LHX3 and SOX2 may contribute to the development of the inner ear and the anterior pituitary. PMID: 18407919
Although the LIM interaction domain of Ldb1 (Ldb1(LID)) and Isl1(LBD) share low levels of sequence homology, X-ray and NMR structures reveal that they bind Lhx3 in an identical manner, that is, Isl1(LBD) mimics Ldb1(LID). PMID: 18583962
Existence of cervical vertebral malformations are revealed, responsible for the rigid neck and the development of scoliosis in LIM3 mutated patients. PMID: 19126629
The Lhx3 gene encodes two isoforms, LHX3a and LHX3b, that differ in their amino-terminal sequences. A novel LHX3 protein (M2-LHX3) is identified and it is determined that this molecule is generated by an internal translation initiation codon. PMID: 11470784