Recombinant Human Interleukin-12 receptor subunit beta-2 (IL12RB2), partial

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中文名稱(chēng)：

人IL12RB2重組蛋白
貨號(hào)：

CSB-YP859518HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥1500
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

IL12RB2
Uniprot No.：

Q99665
別名：

I12R2_HUMAN; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2; IL12 receptor beta 2; IL12R beta2; IL12RB2; Interleukin 12 receptor beta 2; Interleukin 12 receptor beta 2 chain; Interleukin-12 receptor subunit beta-2; RP11 102M16.1
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Extracellular Domain
來(lái)源：

Yeast
分子量：

69.7kDa
表達(dá)區(qū)域：

24-622aa
氨基酸序列

KIDACKRGDVTVKPSHVILLGSTVNITCSLKPRQGCFHYSRRNKLILYKFDRRINFHHGHSLNSQVTGLPLGTTLFVCKLACINSDEIQICGAEIFVGVAPEQPQNLSCIQKGEQGTVACTWERGRDTHLYTEYTLQLSGPKNLTWQKQCKDIYCDYLDFGINLTPESPESNFTAKVTAVNSLGSSSSLPSTFTFLDIVRPLPPWDIRIKFQKASVSRCTLYWRDEGLVLLNRLRYRPSNSRLWNMVNVTKAKGRHDLLDLKPFTEYEFQISSKLHLYKGSWSDWSESLRAQTPEEEPTGMLDVWYMKRHIDYSRQQISLFWKNLSVSEARGKILHYQVTLQELTGGKAMTQNITGHTSWTTVIPRTGNWAVAVSAANSKGSSLPTRINIMNLCEAGLLAPRQVSANSEGMDNILVTWQPPRKDPSAVQEYVVEWRELHPGGDTQVPLNWLRSRPYNVSALISENIKSYICYEIRVYALSGDQGGCSSILGNSKHKAPLSGPHINAITEEKGSILISWNSIPVQEQMGCLLHYRIYWKERDSNSQPQLCEIPYRVSQNSHPINSLQPRVTYVLWMTALTAAGESSHGNEREFCLQGKAN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

N-terminally 6xHis-tagged recombinant human IL12RB2 is produced by expressing the gene fragment encoding the amino acids 24-622 of the human IL12RB2 in the yeast and fusing the 6xHis-motif to the resulting protein. Its purity is greater than 90% determined by SDS-PAGE. It migrated to the molecular weight band of about 66-90 kDa on the gel. The target protein IL12RB2 is a subunit of the IL-12 receptor complex, which is co-expressed with IL12RB1 to form the high-affinity IL-12 binding sites and to reconstitute IL-12-dependent signaling. The up-regulation of IL12RB2 is associated with numerous infectious diseases such as Crohn's disease and leprosy.
Datasheet & COA：

Please contact us to get it.

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■ 常見(jiàn)問(wèn)題解答

I have a customer question about item CSB-YP859518HU Recombinant human Interleukin-12 receptor subunit beta-2. I recently purchased lot # 03363.
It stated the protein is his-tagged however the amino acid of the recombinant protein shown in the page didn’t contain His tag. We also tried western blot in house and we couldn’t detect the his tag. Therefore, I want to clarity if the protein is His tagged.”
Is this protein His tagged? Do you have data to verify that the His-tag is present on Lot # 03363? If so, could you please send the testing data that shows the His tag is present?

Thanks for your inquiry，We have test the protein of the same lot , and we have get the tag . Attach pls find the result.

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靶點(diǎn)詳情

功能：

Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
基因功能參考文獻(xiàn)：
1. The Cox proportional hazard models revealed that IL12Rb2 and p38MAPK predicted a long OS. To the best of our knowledge, the present study is the first to reveal a close association between IL12Rb2 and p38MAPK, and their possible function in nonsmall cell lung cancer progression PMID: 29956791
2. Tumor cell differentiation is associated with TILs' expression of IL-12Rbeta2, and an IL-12Rbeta2(+) TIL ratio >/=35%) indicates favorable prognosis in LC PMID: 29103744
3. inhibition of the H3K27 demethylase JMJD3 in naive CD4 T cells demonstrates how critically important molecules required for T cell differentiation, such as JAK2 and IL12RB2, are regulated by H3K27me3. PMID: 28947543
4. this study identified susceptibility single nucleotide polymorphisms in IL12RB2 with Behcet's disease in Han Chinese PMID: 27464962
5. In ankylosing spondylitis, conditional analysis identified rs11209032 as the probable causal single-nucleotide polymorphism within a 1.14 kb putative enhancer between IL23R and IL12RB2. The rs11209032 single-nucleotide polymorphism downstream of IL23R forms part of an enhancer, allelic variation of which may influence Th1-cell numbers. PMID: 26916345
6. study confirmed the relationship of IL12RB2 polymorphisms with primary biliary cholangitis (PBC) susceptibility; provided evidence that IL12RB2 polymorphisms are associated with liver cirrhosis and an increased concentration of disease-specific anti-mitochondrial antibodies in sera of PBC patients PMID: 28299343
7. this study shows that single nucleotide polymorphisms of the IL23R-IL12RB2 region are associated with Behcet's disease in a Northern Chinese Han population PMID: 27660093
8. IL12RB2 polymorphisms correlate with risk of lung adenocarcinoma. PMID: 26547104
9. The effects of rs3762315 and rs3762316 single nucleotide polymorphisms in 5' flanking region on IL12RB2 transcription PMID: 26552659
10. Estrogen receptor beta 2 regulates IL12RB2 expression via p38 MAPK signaling and inhibits non-small-cell lung cancer proliferation and invasion. PMID: 25695486
11. IL12RB2 polymorphism is associated with systemic lupus erythematosus in the Chinese population. PMID: 25720506
12. results suggest the rs924080 risk A allele does not affect baseline expression of IL-12RB2, IL-23R, IFN-gamma and TNF-alpha but enhances IL-23R and TNF-alpha expression capacity in response to LPS stimulation; the A allele appears to enhance baseline IL-6 production PMID: 24434271
13. a differential regulation pattern for genes solely expressed in Th17 cells (IL17A and CCL20) compared to genes expressed in both Th17 and Th1 cells (IL23R and IL12RB2), where high levels of promoter methylation are correlated to near zero PMID: 22715389
14. Polymorphisms present in the promoter region of IL12RB2 may not be associated with susceptibility to leprosy or its clinical forms. PMID: 24486579
15. ERbeta2 and IL-12Rbeta5 had longer OS. PMID: 23677568
16. Data indicate that four of the IL-12Rbeta2 variants, N271Y, R313G, A604V and L808R showed reduced IL-12 responses compared to the wild type variant. PMID: 23911393
17. Association of IL12RB2 rs6679356 polymorphism with the age of type 1 diabetes mellitus onset suggests that this gene plays a role in defining the time of disease onset. PMID: 23152861
18. the -237 polymorphic site in the 5' promoter region of the IL-12Rbeta2 (SNP ID: rs11810249) gene associated with the AP-4 transcription motif GAGCTG PMID: 22509293
19. data clearly support the IL12RB2 genetic association with systemic sclerosis (SSc), and suggest a relevant role of the interleukin 12 signaling pathway in SSc pathogenesis. PMID: 22076442
20. Our results support a potential influence of the rs3790567 IL12RB2 polymorphism in the pathogenesis of giant cell arteritis. PMID: 21285166
21. significant interaction between the IL-12RB1 and IL-12RB2 genes contributes to a 4-fold increased risk for developing extrinsic atopic dermatitis PMID: 20060272
22. STATE OF ART REVIEW. IL-12Rb2 gene is not expressed in EBV-transformed normal B-lymphocytes and in Burkitt's lymphoma B-cell lines. PMID: 11940489
23. upregulated in Il-12 augmented T cell activation of mononclear leukocytes from HIV seropositive individuals PMID: 12804004
24. promoter activity is increased in case of the -465G allele, disrupting the intact GATA site PMID: 15140029
25. Study showed recombinant human interleukin-12 (rhIL-12) safe/well tolerated in healthy subjects given single doses (1 up to 8 ug) subcutaneously. Interferon-gamma and, signal transducer and activator of transcription may be useful biomarkers of rhIL-12. PMID: 15901746
26. Epigenetic silencing of IL-12Rbeta2 is a frequent event in lung cancers. Aberrant methylation of this gene seems to be a useful predictor of long-term outcome for adenocarcinoma of the lung. PMID: 17602269
27. frequencies of variant alleles were significantly higher in aggressive periodontitis patients as compared with healthy controls or chronic periodontitis patients PMID: 18353079
28. IL-12R beta 2 directly restrains multiple myeloma cell growth PMID: 18474725
29. These results suggest that these SNPs in IL12RB2 have differential effects on cellular activation of T cells and NK cells. PMID: 18771340
30. Single Nucleotide Polymorphism in interleukin 12 receptor beta 2 is associated with susceptibility to radiation dermatitis in breast cancer. PMID: 18927311
31. Our data show significant associations between primary biliary cirrhosis and common genetic variants at the HLA class II, IL12A, and IL12RB2 loci PMID: 19458352
32. Studies established the concept that the IL-12Rbeta2 gene is a gatekeeper from cancer. PMID: 19720917
33. In the generation of cell-mediated immunity against intracellular infection, the expression and function of the IL-12 receptor beta 2 chain correlate with the response of Th1-type cells to Mycobacterium leprae antigen. PMID: 11441083
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亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Type I cytokine receptor family, Type 2 subfamily
組織特異性：

Isoform 2 is expressed at similar levels in both naive and activated T-cells.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 5972

OMIM: 601642

KEGG: hsa:3595

STRING: 9606.ENSP00000262345

UniGene: Hs.479347