Recombinant Human Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3)

中文名稱：

人IGF2BP3重組蛋白
貨號：

CSB-EP011092HU
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

IGF2BP3
Uniprot No.：

O00425
別名：

(IGF2 mRNA-binding protein 3)(IMP-3)(IGF-II mRNA-binding protein 3)(KH domain-containing protein overexpressed in cancer)(hKOC)(VICKZ family member 3)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

69.7 kDa
表達區域：

1-579aa
氨基酸序列

MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAIIGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
基因功能參考文獻：
1. Data show that insulin-like growth factor-II mRNA-binding protein 2 and 3 (IMP2/3)-miR-200a-progesterone receptor axis represents a double-negative feedback loop and serves as a new potential therapeutic target for the treatment of Triple-negative breast cancer (TNBC). PMID: 29217458
2. THADA fusion is a mechanism of IGF2BP3 activation and IGF1R signaling in thyroid cancer. PMID: 28193878
3. High expression of IMP3 in adult mature testicular teratomas supports their malignant nature. PMID: 24605891
4. tubal secretory cells with IMP3 signatures showing growth advantage could potentially serve as a latent precancer biomarker for tubal or pelvic serous carcinomas in women. PMID: 25014991
5. These results suggested that pancreatic carcinogenesis involves an increased IMP3 mRNA expression, and it may become valuable diagnostic and prognostic markers as well as potential therapeutic targets for pancreatic cancer. PMID: 25183049
6. the combination of p16/IMP3 expression was found to improve the discrepant results between cervical cytologic and histologic diagnoses PMID: 23923073
7. High IMP3 expression is associated with hepatocellular carcinoma. PMID: 24647926
8. Our results strengthen the association of IMP3 with the regulation of epithelial to mesenchymal transition in breast cancers PMID: 25031719
9. Post-transcriptional regulation of cyclins D1, D3 and G1 and proliferation of human cancer cells depend on IMP-3 nuclear localization. PMID: 23812426
10. IMP3 upregulates HMGA2 mRNA by opposing its interaction with Ago2/let-7. PMID: 24703842
11. The results of this study indicate that IMP3 was overexpressed in sacral chordoma and this expression was associated with tumor invasion and recurrence. PMID: 24135846
12. IMP3 may be involved in the process and progression of high-grade serous carcinoma of the fallopian tube. PMID: 25038792
13. Absence of IMP3 expression is associated with a significantly reduced risk of lymphovascular invasion in pT1 esophageal adenocarcinoma PMID: 24726263
14. Diffuse IMP3 expression (>25%) in MAC from patients with gastric adenocarcinoma was associated with shorter survival. PMID: 24457229
15. This review focuses on the studies of IMP3 expression in different cancers and emphasizes the potential utility of IMP3 in routine surgical pathology practice. PMID: 24713990
16. IMP3 expression was positive in esophageal adenocarcinoma and Barrett's esophagus with high-grade dysplasia. Barrett's esophagus and esophageal squamous epithelium had no IMP3 expression PMID: 25141312
17. High IMP3 was associated with metastasis in prostate cancer. PMID: 24615121
18. High-grade dysplasia and gastric carcinomas can be distinguished from low-grade dysplasia and inflammatory lesions of the gastric mucosa with a high specificity and good sensitivity using a combination of the immunohistochemical markers IMP3 and p53 PMID: 24966917
19. IMP-3 expression is not a useful discriminator between dysplastic nevi and melanoma nor a good prognostic marker in melanoma. PMID: 26403409
20. The specificity for IMP3, GLUT-1, and EMA for differentiating malignant mesotheliomas and benign mesothelial proliferations was 100% PMID: 25376377
21. expression in preoperative biopsy material predictive of perineural invasion in patients with oral squamous cell carcinoma PMID: 27863864
22. IMP3 appears to play an important role in tumor invasion in patients with lung adenocarcinoma and may serve as a useful prognostic biomarker in these patients. PMID: 26608347
23. IMP3 represents a biomarker of unfavorable prognosis for clear cell carcinomas of the ovary, even in stage I patients. PMID: 26837693
24. results suggest that IMP3 facilitates pancreatic ductal adenocarcinoma progression by enhancing the pro-metastatic behaviour of tumour cells PMID: 25886367
25. EGF stimulates IMP-3 expression. PMID: 26386725
26. MP3 may play a certain role in hydatidiform moles progression, and it may serve as a prognostic biomarker for this disease. PMID: 27238915
27. Testing for a combination of elevated IMP3 and Lin28B levels could further facilitate the identification of a patient subgroup with the worst ovarian cancer prognosis. PMID: 26158423
28. we show that SOX2 is a transcriptional target of SLUG. These data establish a novel mechanism of breast tumor initiation involving IMP3 and they provide a rationale for its association with aggressive disease and poor outcome. PMID: 25982283
29. This is the first study evaluating the IMP3 immunohistochemical expression in Phyllodes tumor. Its expression correlates with histological grade and could be used in the differential diagnosis of fibroepithelial tumors and in prognostication. PMID: 26353850
30. IMP3 is a reliable marker for the diagnosis of colorectal cancer in endoscopic biopsies PMID: 28412210
31. Positive expression of IMP3 in spinal giant cell tumor was associated with increased microvessel density and expression of IGF2. PMID: 25740666
32. Tissue overexpression of IMP3 might be used as a predictor of advanced disease or lymph node metastasis, and is associated with poorer prognosis in gastric cancers. PMID: 28011483
33. IMP-3 has a role in promoting migration and invasion of melanoma cells by modulating the expression of HMGA2 and predicts poor prognosis in melanoma PMID: 25380351
34. The role of IMP3 as a marker of poor outcome in gliomas PMID: 25695077
35. IMP3 directly interacts with ULBP2 mRNA, leading to ULBP2 transcript destabilization and reduced ULBP2 surface expression and indirectly downregulates MICB with a mechanism functionally distinct from that of ULBP2. PMID: 26982091
36. This study demonstrated that Genes overexpressed in Pilomyxoid Astrocytoma vs. Pilocytic Astrocytoma, ranked according to fold-change, included developmental genes H19, DACT2,COL2A1; COL1A1 and IMP3. PMID: 25521223
37. Describe chromosome 12p abnormalities and IMP3 expression in prepubertal pure testicular teratomas. PMID: 26826410
38. This study aimed to demonstrate the usefulness of mRNA expression levels of IMP3 in brush cytology specimens combined with cytology for the diagnosis of malignant biliary stricture PMID: 27399126
39. IMP-3 and its protein partners ILF3/NF90 and PTBP1 bind to the 3'UTRs of the cyclin mRNAs and protect them from the translational repression induced by miRNA-dependent recruitment of AGO2/GW182 complex in human cancer cells. PMID: 27840950
40. IMP3 promotes renal cell carcinoma cell migration and invasion by activation of NF-small ka, CyrillicB pathway. PMID: 25919292
41. Overexpression of IMP3 and EGFR were significantly associated with shorter periods of metastasis-free and disease-free survival of malignant phyllodes tumor of the breast. PMID: 27137988
42. S100P achieved 96.4% sensitivity, 93.3% specificity, 98.2% PPV, 87.5% NPV and 95.8% total accuracy, while IMP3 achieved 91.2% sensitivity, 86.7% specificity, 96.2% PPV, 72.2% NPV and 90.3% total accuracy for pancreatic ductal adenocarcinoma (PDA).Both markers were sensitive and specific for diagnosis of PDA. PMID: 27839709
43. CK5/6, IMP3 and TTF1 immunostaining appears to be useful to improve the accuracy of cytological diagnoses between reactive mesothelial cells, metastatic adenocarcinoma of lung and non-lung origin in pleural effusion PMID: 25337222
44. Our study clearly demonstrated differentiation-dependent expression of IMP3 in chondrosarcoma, and suggests IMP3 as a novel marker for differentiating problematic cases of enchondroma from well-differentiated chondrosarcomas PMID: 26948096
45. It was concluded that IMP3 and nucleophosmin cannot be a routine diagnostic marker for discrimination of papillary carcinomas and benign lesions. IMP3 positive staining was scarce in IMP3 positive papillary carcinomas although specifity of IMP3 is 100% PMID: 25389240
46. MP3 expression in biopsy specimens could be used to predict lymph node metastasis and TNM stage in colorectal cancer patients PMID: 26617820
47. IGF2BP3 influences a malignancy-associated RNA regulon by modulating miRNA-mRNA interactions. PMID: 27210763
48. IGF2BP3 as a human ontogenic master switch that restricts megakaryocyte development by modulating a lineage-specific P-TEFb activation mechanism. PMID: 28481226
49. Results suggest that IGF2BP3 promotes eIF4E-mediated translational activation through the reduction of EIF4E-BP2 via mRNA degradation, leading to enhanced cell proliferation. PMID: 26522719
50. We compressively revealed the oncogenic role of IGF2BP3 in gastric tumorigenesis and confirmed its activation is partly due to the silence of miR-34a. Our findings identified useful prognostic biomarker and provided clinical translational potential. PMID: 28399871
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亞細胞定位：

Nucleus. Cytoplasm. Note=Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. In response to cellular stress, such as oxidative stress, recruited to stress granules.
蛋白家族：

RRM IMP/VICKZ family
組織特異性：

Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic
數據庫鏈接：

HGNC: 28868

OMIM: 608259

KEGG: hsa:10643

STRING: 9606.ENSP00000258729

UniGene: Hs.700696