Recombinant Human Hyaluronidase PH-20 (SPAM1)

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中文名稱：

人SPAM1重組蛋白
貨號：

CSB-EP022471HU
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP022471HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) SPAM1.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP022471HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) SPAM1.
其他：

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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

SPAM1
Uniprot No.：

P38567
別名：

epididymis secretory sperm binding protein Li 96n; HEL-S-96n; HYA1; HYAL PH-20; HYAL PH20; Hyal-PH20; HYAL1; HYAL3; HYAL5; HYALP_HUMAN; Hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; MGC26532; PH-20 Hyaluronidase; PH20; PH20 Hyaluronidase; SPAG15; SPAM-1; Spam1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); Sperm adhesion molecule 1; Sperm surface protein PH-20; Sperm surface protein PH20; zona pellucida binding
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

67.9kDa
表達區域：

36-490aa
氨基酸序列

LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Recombinant Human Hyaluronidase PH-20(SPAM1) is a partial length protein expressed with N-terminal 6xHis-SUMO-tagged in the E.coli. Its expression region corresponds to 36-490aa of human SPAM1 protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band of 67.9 kDa on the gel. This recombinant SPAM1 protein may be used to synthesize antibodies against SPAM1.
SPAM1 is involved in sperm-egg adhesion. Upon fertilization, sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Diseases associated with SPAM1 include Mucopolysaccharidosis, Type Ix and Aqueous Misdirection.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
基因功能參考文獻：
1. Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID: 25209425
2. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID: 24778442
3. HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID: 23247813
4. The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID: 23209833
5. PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID: 23463525
6. SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID: 12932297
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亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

Glycosyl hydrolase 56 family
組織特異性：

Testis.
數據庫鏈接：

HGNC: 11217

OMIM: 600930

KEGG: hsa:6677

STRING: 9606.ENSP00000345849

UniGene: Hs.121494