E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Liquid or Lyophilized powder
Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
基因功能參考文獻:
Mutation in GHRHR is associated with pituitary diseases. PMID: 29277338
Report GHRH-R labelling of apocrine glands and neoplastic epithelium in adnexal tumours and apocrine glands of the skin. PMID: 29895126
Single nucleotide variant in GHRHR gene is associated with isolated growth hormone deficiency. PMID: 28910730
GHRHR mutation is associated with growth hormone deficiency. PMID: 28525353
The pP79L mutation is associated with the compromise in function, with the residual partial activity explaining the mild phenotype. PMID: 27501283
This is the first report to demonstrate the GHRH-R codon 72 mutation in Sri Lankan patients with confirmed growth hormone deficiency. PMID: 27031974
This study shows that GHRH-R is expressed by the majority of malignant mixed Mullerian tumors in both epithelial and mesenchymal components PMID: 26535981
Identification of new missense mutations and an insertion mutation for the GHRHR gene in patients with isolated growth hormone deficiency. PMID: 25541890
A reduced stature in older subjects heterozygous for the c.57+1G>A GHRHR mutation, indicating different effects of heterozygosis through lifespan. PMID: 25761575
we found that pGHRH-R and its main splice variant are expressed in thyroid tissue and was upregulated in tumor cells compared to normal thyroid cells PMID: 25752763
Indel mutation in the growth hormone releasing hormone receptor gene is associated with isolated growth hormone deficiency. PMID: 25153028
These preliminary results suggest a greater than average GHRH-R expression in invasive lobular carcinomas and invasive ductal carcinomas associated with casting-type calcifications on the mammogram. PMID: 24479854
Compound heterozygous mutations of the growth hormone-releasing hormone receptor gene is associated with isolated growth hormone deficiency PMID: 23602557
Genu valgum was more prevalent in growth hormone deficiency GHRHR mutation patients than controls. PMID: 24057284
We report a novel splice-disrupting mutation in GHRHR in 2 siblings and provide evidence that all c.57+1G>A (IVS1+1G>A) mutant chromosomes have the same haplotype ancestor PMID: 23052699
The study established the GHRHR gene sequence variation map in isolated severe growth hormone deficiency patients and normal adult height. PMID: 22489751
bone quality and metabolism and its correlation with insulin sensitivity in subjects heterozygous for a null mutation in GHRHR PMID: 21995288
GHRH-R and Hsp90 were found to be independent predictive factors of histopathological response to neoadjuvant RCT. PMID: 22160161
study describes a new frame shift mutation in the coding sequence of exon 4 (corresponding to the extracellular domain of the receptor)(c.391delG) in the GHRHR in a family with isolated GH deficiency PMID: 21816782
This chapter reviews the biology of the GHRHR, the mutations that affect its gene and their effects in homozygous and heterozygous individuals. PMID: 20374725
The homozygous GHRHR mutation was rare, being detected in only one Japanese isolated GH deficiency family. PMID: 21044116
The endometriotic stromal cells (ESCs) and transformed human ESCs, but not normal endometrial tissues, expressed GHRH-R mRNA. PMID: 19524226
These results show, for the first time, the activation of the MAPKs cascade by the splic variant (SV1) receptor. PMID: 19897610
Longevity in untreated congenital growth hormone deficiency due to a homozygous mutation in the GHRH receptor gene. PMID: 19965916
To ascertain whether (splice variant) SV1 mediates mitogenic effects on nonpituitary tissues, we expressed SV1 in 3T3 mouse fibroblasts and studied the properties of the transfected cells PMID: 11773624
results demonstrate that mutations in the GHRHR are not limited to the coding sequence and that promoter mutations that impair Pit-1 binding can reduce expression of the GHRHR gene PMID: 11875102
human GHRHR isoforms are found in experimental prostate neoplasms in mice; this could explain the effects of GHRH antagonists on growth of prostate cancer PMID: 12126741
expression of mRNA and splice variants of this receptor in human malignant bone tumors PMID: 12220726
an examination of the possible clinical significance of a polymorphism in the gene of this receptor: involvement in acromegaly PMID: 12220735
mutations in the GHRH receptor gene make abnormalities of this gene one of the most common causes of of isolated growth hormone deficiency--REVIEW PMID: 12424433
Compound heterozygotes for two previously undescribed mutations in the GHRHR that are predicted to cause complete lack of functional GHRHR protein: a nonsense mutation in codon 43 (Q43X), and a splice mutation at the beginning of intron 3 (IVS3+1G-->A). PMID: 12444890
GHRH receptor may be associated with carcinogenesis PMID: 12867592
autocrine stimulatory loop between GHRH and SV1 variant of GHRH receptor in primary cancers. PMID: 15362970
GHRH-R was demonstrated in prostate and breast carcinomas, opening a variety of possibilities for the use of GHRH antagonists in the treatment of prostatic and mammary tumors. PMID: 15944917
analysis of receptors for growth hormone-releasing hormone in human osteosarcomas and Ewing's sarcomas PMID: 16820890
Heterozygosity for a null GHRHR mutation is not associated with reduction in adult stature or in serum IGF-I but is associated with changes in body composition and possibly an increase in insulin sensitivity PMID: 17356054
Size of individual abdominal organs is influenced in different ways by severe and congenital lack of GH due to a GHRH-R mutation. PMID: 18034778
the expression of GHRH and its tumoral receptor SV1 in primary human melanomas and dysplastic nevi by immunohistochemistry. None of the specimens tested expressed GHRH PMID: 18255167
targeting the GHRH receptor may be a therapeutic option in Triple-negative breast cancers PMID: 18629632
Gsp mutations up-regulate GHRHR mRNA expression in GH-secreting pituitary adenoma cells & desensitize the adenoma cells to GHRH in terms of their GHRHR mRNA expression. PMID: 19029774
GHRHR might be one of the most important genes so far identified affecting normal variation in human height. PMID: 19209235
Mutations in GH1 and GHRHR were identified in 41 patients from 21 pedigrees, representing 11.1% of our cohort with a higher prevalence of mutations in familial cases (38.6%) and in consanguineous pedigrees (75%) of congenital growth hormone deficiency. PMID: 19567534
Common variants of the GNRH1 and GNRHR genes are not associated with risk of invasive breast cancer in Caucasians PMID: 19640273
These results provide strong evidence that the splicing mutation IVS8+1G>A of growth hormone-releasing hormone receptor is a cause of pituitary dwarfism in the Chinese family. PMID: 19733620