Recombinant Human Glucokinase regulatory protein (GCKR)

^{In
Stock}

中文名稱：

人GCKR重組蛋白
貨號：

CSB-YP622767HU
說明書：

MSDS Datasheet
規格：

￥1500
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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相關產品
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

GCKR
Uniprot No.：

Q14397
別名：

FGQTL5; GCK; GCKR; GCKR_HUMAN; GKRP ; GLRE; glucokinase (hexokinase 4) regulator; Glucokinase; Glucokinase regulator; Glucokinase regulatory protein; Hexokinase 4 regulator; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; OTTHUMP00000158533
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

Yeast
分子量：

70.7kDa
表達區域：

1-625aa
氨基酸序列

MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答

Can you provide details on GCKR Recombinant Protein? Notably, the sequence including tags and linkers. I would like to know if there are any additional tags, where the his is located, and if there is a cleavage sequence (eg TEV).

Very nice to receive your inquiry. The product details are shown in the section of specification.
2) This is a full-length protein. If you are asking whether there is cleavage sequence between the tag and the target protein sequence, then the answer is "NO".
If you want to remove the tag, we could change new vector and try to remove the tag.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.If we fail in removing the tag, we won’t charge for any extra cost.

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靶點詳情

功能：

Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
基因功能參考文獻：
1. The results suggest that the GCKR and G6PC2 genes may contribute to the risk of type 2 diabetes independently and/or in an interactive manner in the Han Chinese population. PMID: 30055620
2. Results show that GCKR was associated with hypertriglyceridemia in Mexican Mestizos but not in Mexican Amerindians. PMID: 30176313
3. The results of this population-based study provide evidence for a relationship between lipid regulatory gene polymorphisms including GCKR (rs780094), GCKR (rs1260333), FADS (rs174547), and MLXIPL (rs3812316) with dyslipidemia in an Iranian population. PMID: 29858861
4. genome-wide association study in US: Data suggest, among white subjects, an SNP in RCN3 (rs34459162) and a missense mutation in GCKR (rs1260236) are associated with serum levels of glycated albumin; among black subjects, an intergenic SNP in PRKCA (rs2438321) is associated with fructosamine levels and an intronic variant in PRKCA (rs59443763) is associated with glycated albumin levels. (RCN3 = reticulocalbin-3) PMID: 29844224
5. Interaction of any alcohol exposure with GCKR (rs780094) and A1CF (rs10821905) influenced the risk of gout in Europeans PMID: 28679452
6. GCKR rs780094-C is associated with increased risk of gestational diabetes mellitus.[meta-analysis] PMID: 29410004
7. Mutations in the genes glucokinase regulatory protein (GCKR), RNase L (RNASEL), leukocyte immunoglobulin-like receptor 3 (LILRA3), and dynein axonemal heavy chain 10 (DNAH10) segregated with elevated HDLc levels in families, while no mutations associated with low HDLc. PMID: 24891332
8. rs1260326 T-allele was associated with plasma lactate in European-Americans and African-Americans. PMID: 26433129
9. Patients with moderately controlled type 2 diabetes carrying two T alleles displayed higher plasma triglycerides levels than homozygous carriers of the C allele, whereas no differences were observed in healthy individuals. PMID: 27660121
10. GCKR association with adiposity and the risk of the nonalcoholic fatty liver disease PMID: 28436986
11. The existence of a liver-specific FOXA2-regulated transcriptional enhancer at an intronic T2D locus represented by rs780094, rs780095, and rs780096 SNPs that increases GCKR expression is demonstrated. PMID: 28683826
12. GCKR Leu446 may influence FGF21 expression via its ability to increase glucokinase (GCK) activity. This can lead to enhanced FGF21 expression via elevated fatty acid synthesis, consequent to the inhibition of carnitine/palmitoyl-transferase by malonyl-CoA, and via increased glucose-6-phosphate-mediated activation of the carbohydrate response element binding protein, known to regulate FGF21 gene expression. PMID: 28385800
13. The rs780094 [1.34 (1.21-1.49); p = 8.57 x 10(-8) ] on chr 2 at the glucokinase regulatory protein (GCKR) locus was similarly significantly associated, while the rs10911205 [1.29 (1.16-1.44); p = 3.52 x 10(-6) ] on chr1 at the laminin subunit gamma-1 (LAMC1) locus showed suggestive association with disease. PMID: 27599772
14. Carriers of the C allele of rs780094 were 1.41 (odds ratio, 95% CI, 0.97-2.03) times more likely to develop Gestational Diabetes. PMID: 27554451
15. Among Mexicans, the PNPLA3 (rs738409), LYPLAL1 (rs12137855), PPP1R3B (rs4240624), and GCKR (rs780094) polymorphisms may be associated with a greater risk of chronic liver disease among overweight adults. PMID: 27752939
16. The present study explored the association between a common variant of the glucokinase regulator (GCKR) gene and metabolic syndrome and its related traits in Taiwanese adolescents PMID: 26799416
17. This study shows that in patients with type 2 diabetes who were not selected for liver abnormalities, liver fat content was related to GCKR rs1260326 polymorphism PMID: 25976242
18. Lifestyle Intervention for Weight Loss and Cardiometabolic Changes in the Setting of Glucokinase Regulatory Protein Inhibition: Glucokinase Regulatory Protein-Leu446Pro Variant in Look AHEAD PMID: 26578543
19. The GCKR rs780092 variant showed opposite-directional associations with type 2 diabetes and hypertriacylglycerolaemia in a Chinese population. PMID: 26515422
20. The AA genotype of the GCKR rs780094 polymorphism may increase the risk of T2D among ethnic Uygurs from Xinjiang. PMID: 27455017
21. Two new polymorphisms associated with gall bladder disease and obesity in women have been identified, GCKR rs1260326, and TTC39B rs686030. PMID: 25920552
22. We found that the PPARGC1A and PNPLA3 variants, but not the GCKR variant, were associated with NASH. PMID: 27015186
23. Disruption of the GKRP-glucokinase complex is a promising new antidiabetic target, since it results in lower plasma glucose levels without inducing hypoglycemia[review] PMID: 26432016
24. this is the first evidence that n-3 LC-PUFAs may modulate the impact of the GCKR rs1260326 polymorphism on TG concentrations in adolescents. Several molecular mechanisms, in link with glucose uptake, could explain these findings PMID: 26136510
25. SNPs in SORT1, CETP and GCKR were individually associated with lipid level variations in Algerian population. PMID: 26261636
26. interaction effects between GCKR genetic variants and the dietary MUFA:SFA ratio on lipid levels PMID: 26291577
27. rs1260326 in GCKR significantly associates with alpha-1 antitrypsin levels. PMID: 26174136
28. GCKR polymorphisms might be independent predictors of survival in metastatic gastric cancer patients treated with first-line EOF chemotherapy PMID: 26115082
29. The AA genotype in maternal GCKR rs780094 is associated with an increased risk for neural tube defects and spina bifida in the Chinese population. PMID: 25369983
30. Single-nucleotide polymorphisms alleles near GCKR that were associated with higher coffee consumption. PMID: 25288136
31. GCKR variants across the allelic spectrum have effects on glucose and lipid homeostasis. [Review] PMID: 25692341
32. GCKR rs1260326 gene variant, which is associated with greater glycolysis, increases hepatic De Novo Lipogenesis. PMID: 26043229
33. Polymorphisms in GCKR, SLC17A1 and SLC22A12 were associated with phenotype gout in Han Chinese males. PMID: 26290326
34. meta-analysis provides evidence of significant association between GCKR rs780094 and risk of nonalcoholic fatty liver disease PMID: 25167786
35. GCKR rs780094 variants modulate uric acid concentrations in Chinese males. PMID: 25283508
36. Individuals who are homozygous TT at rs1260326 of the GCKR gene have higher triglyceride, total and LDL cholesterol levels regardless of the presence of GCK mutations. PMID: 24918535
37. Despite evidence for their collective functional and clinical relevance, results emphasize the low predictive value of rare GCKR variants in individuals and the complex heritability of lipid traits. PMID: 24879641
38. genetic association studies in Japan: Data suggest that SNPs in GCKR (rs780094), TRIB1 (tribbles pseudokinase 1, rs2954021), and PNPLA3 (patatin-like phospholipase domain containing 3, rs738409) are associated with nonalcoholic fatty liver disease. PMID: 24785259
39. Results showed that the GCKR rs1260326 polymorphism is significantly associated with the risk of chronic kidney disease (CKD) in Japanese individuals, and found a possible significant interaction between this polymorphism and current smoking on CKD risk. PMID: 24535998
40. GCKR and PNPLA3 genes interact to result in increased susceptibility to NAFLD. PMID: 23800943
41. Data indicate that in non-alcoholic fatty liver disease (NAFLD), glucokinase regulatory protein (GCKR) rs780094 C>T is associated with the severity of liver fibrosis and with higher serum triglyceride levels. PMID: 24498332
42. GCKR rs780094 was associated with TG, HDL-C, and HbA1c levels, as well as with CIMT in Japanese community-dwelling men. PMID: 23989113
43. genetic association studies in population in Norfolk, England: Data suggest that an SNP in GCKR (rs780094) is associated with risk of cardiometabolic diseases (cardiovascular diseases; diabetes type 2) in the population studied. PMID: 24804806
44. genetic polymorphism is associated with Factor VII and with plasma viscosity PMID: 24178511
45. The GCKR rs780093 polymorphism is a risk factor for coronary heart disease in individuals aged 65 and older. PMID: 24385677
46. RESULTS: rs1260326 in GCKR (beta=1.30, P = 3.23E-03), rs17319721 in SHROOM3 (beta = -1.28, P-value = 3.18E-03) and rs12917707 in UMOD (beta = 2.0, P-value = 8.84E-04) were significantly associated with baseline estimated GFR PMID: 23586973
47. genetic association studies in Han Chinese population in Taiwan: Data suggest that an SNP in GCKR (rs780094) is associated with increased susceptibility/risk of nonalcoholic fatty liver disease in the population of obese children studied. PMID: 24477042
48. Findings from middle-aged to older adults confirm associations between rs1260326 GCKR and triglycerides and glucose, suggest gene-environment interactions, but do not provide evidence that its relevance extends to cognitive and physical capability. PMID: 23894584
49. Some, but not all, associations between variants in NCAN, lysophospholipase-like 1, GCKR, and PPP1R3B with hepatic steatosis (with and without increased ALT level) were significant within subpopulations. PMID: 23416328
50. Our observations provide a model for the regulation of GCK-GKRP complex stability by sugar phosphates involving a subtle reorganization of the GKRP scaffold. PMID: 23957911
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亞細胞定位：

Cytoplasm. Nucleus. Mitochondrion.
蛋白家族：

GCKR family
組織特異性：

Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
數據庫鏈接：

HGNC: 4196

OMIM: 600842

KEGG: hsa:2646

STRING: 9606.ENSP00000264717

UniGene: Hs.89771