Recombinant Human E3 ubiquitin-protein ligase NEDD4 (NEDD4), partial
-
中文名稱：人NEDD4重組蛋白
-
貨號：CSB-EP015681HU-B
-
說明書：
-
規(guī)格：
-
來源：E.coli
-
共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
-
其他：
產(chǎn)品詳情
-
純度：>85% (SDS-PAGE)
-
基因名：
-
Uniprot No.：
-
別名：Cell proliferation-inducing gene 53 protein; E3 ubiquitin protein ligase Nedd4; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; KIAA0093; NEDD 4; NEDD-4; Nedd4; NEDD4_HUMAN; Neural precursor cell expressed developmentally down regulated 4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; PIG53; receptor-potentiating factor 1; RPF1
-
種屬：Homo sapiens (Human)
-
蛋白長度：Partial
-
蛋白標(biāo)簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
-
儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
-
保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
-
注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
Datasheet ：Please contact us to get it.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
-
功能：E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1.; (Microbial infection) Involved in the ubiquitination of Ebola virus protein VP40 which plays a role in viral budding.
-
基因功能參考文獻(xiàn)：
- NEDD4 mediates the EGFR lung cancer cell migration signaling through promoting lysosomal secretion of cathepsin B. PMID: 29455656
- Data indicate the mechanism for epigenetic regulation in cancer by inducing E3 ubiquitin ligase NEDD4-dependent histone H3 ubiquitination. PMID: 28300060
- We further investigated whether curcumin exerts its antitumor activities via suppressing NEDD4 expression. We found that curcumin reduced the expression of NEDD4 and Notch1 and pAKT, leading to glioma cell growth inhibition, apoptosis, and suppression of migration and invasion. Moreover, deletion of NEDD4 expression enhanced the sensitivity of glioma cells to curcumin treatment PMID: 28627598
- an oncogenic E3 ubiquitin ligase promotes loss of gap junctions and Cx43 degradation in human carcinoma cells. PMID: 28733455
- NEDD4 exerts its oncogenic function partly due to regulation of PTEN and Notch-1 in bladder cancer cells. PMID: 28745938
- In the present study, we identified that the adaptor protein Numb, which is demonstrated to be a novel binding partner of NEDD4-1, plays important roles in controlling PTEN ubiquitination through regulating NEDD4-1 activity and the association between PTEN and NEDD4-1. PMID: 28437168
- NEDD4 is an autophagic E3 ubiquitin ligase that ubiquitylates SQSTM1, facilitating SQSTM1-mediated inclusion body autophagy. PMID: 29021346
- NEDD4 is largely involved in epithelial-mesenchymal transition features and chemoresistance of nasopharyngeal carcinoma cancer cells. PMID: 28379054
- NEDD4 ligase regulates RTP801 and is sensitive to Parkinson disease-associated oxidative stress PMID: 27494837
- Two additional regulators of BST2 constitutive ubiquitylation and sorting to the lysosomes: the E3 ubiquitin ligases NEDD4 and MARCH8, are reported. PMID: 28320822
- SQSTM1 is ubiquitinated by NEDD4 while LC3 functions as an activator of NEDD4 ligase activity. PMID: 28085563
- Our results reveal a novel mechanism of upregulation of NEDD4 expression in pancreatic adenocarcinoma PMID: 28423617
- Describe an autoinhibitory mechanism for NEDD4-1 ubiquitin ligase involving a linker-HECT domain interaction. This intramolecular interaction traps the HECT enzyme in its inactive state and can be relieved by linker phosphorylation. PMID: 28475870
- Insights into links between autophagy and the ubiquitin system showed that LC3B-binding can steer intrinsic NEDD4 E3 ligase activity. PMID: 28470758
- NEDD4-1 recognizes SERTA domain containing proline rich region of p34SEI-1. Residues of NEDD4-1 responsible for direct interaction with p34SEI-1 are identified by NMR titration experiments. PMID: 28666866
- Our results demonstrate that NEDD4 may promote the acquired resistance of non-small-cell lung cancer cells to erlotinib by decreasing phosphatase and tensin homolog deleted on chromosome 10 protein expression. Targeted decrease in NEDD4 expression may be a potential therapeutic strategy for tyrosine kinase inhibitor-resistant non-small-cell lung cancer. PMID: 28714370
- this is the first demonstration that Nedd4-1 regulates hOAT1 ubiquitination, expression, and transport activity through its WW2 and WW3 domains PMID: 27226107
- both Nedd4-1 and Nedd4-2 are important regulators for hOAT1 ubiquitination, expression, and function PMID: 26823285
- Nedd4-1 inhibited Rap2a activity, and promoted the migration and invasion of glioma cells. PMID: 28405688
- We concluded that USP15 attenuates IGF-I signaling by antagonizing Nedd4-induced IRS-2 ubiquitination. PMID: 28126338
- Data suggest a possible molecular mechanism might be that NEDD4 silence led to an increase in PTEN (phosphatase and tensin homologue) expression. PMID: 27467187
- we found that Ndfipl binds to Nedd4-1, and that increased expression of Ndfipl significantly reduced Itch expression. We also found that increased ubiquitination played a role in Ndfipl-mediated processes, and that Ndfipl and a-synuclein interact PMID: 27173227
- Upregulation of long non-coding RNA PAPAS in response to hypoosmotic stress does not increase H4K20me3 because of Nedd4-dependent ubiquitinylation and proteasomal degradation of Suv4-20h2. PMID: 26904956
- Elevated NEDD4 is implicated as the responsible ubiquitin E3 ligase for HSF1 degradation through ubiquitin-proteasome system. Aberrant HSF1 degradation is a key neurodegenerative mechanism underlying alpha-synucleinopathy. PMID: 26503960
- LRAD3 is a component of pathways that function effectively to modulate Itch and Nedd4 auto-ubiquitination and levels. PMID: 26854353
- These results show that the binding of the hNedd4-1 WW3* domain to alpha-hENaC is coupled to the folding equilibrium PMID: 26685112
- Data show that human ABCB1 is a substrate for human NEDD4-1 and identified 8 lysine residues that were ubiquitinated by NEDD4-1. PMID: 26006083
- we demonstrate that the E3 ubiquitin ligase NEDD4 ubiquitinates IFITM3 in cells and in vitro. PMID: 26263374
- Data show that silencing of PMEPA1 protein facilitates the growth of prostate cancer cells and modulates androgen receptor (AR) through NEDD4 ubiquitin protein ligase and PTEN protein. PMID: 25883222
- Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity. PMID: 25879670
- NEDD4 controls the Hippo pathway leading to coordinated cell proliferation and apoptosis PMID: 25692647
- sNEDD4 is a novel metastasis-associated gene, which prevents apoptosis under nutrient restriction conditions. This support the prognostic potential of sNEDD4 for Hepatocellular carcinoma. PMID: 25823820
- Study identifies NEDD4 as a putative oncogene in endometrial cancer that may augment activation of the IGF-1R/PI3K/Akt signaling pathway. PMID: 26193427
- HUWE1 and NEDD4-1 are two E3 ligases that are fundamental enzymes in the post-translational regulation of ABCG1 and ABCG4 protein levels and cellular cholesterol export activity PMID: 26296893
- Studies on Smurf2 and Nedd4 show that the C2 domain has the potential to regulate E3 activity by maintaining the HECT domain in a low-activity state where its ability for transthiolation and noncovalent Ubiquitin binding are impaired. PMID: 25438670
- rs3088077, rs7162435 and rs2303579 of the NEDD4 gene are associated with schizophrenia. PMID: 26037357
- Nedd4 promoted autophagy and was associated with the mTOR signalling pathway in the prostate cancer cells. PMID: 25809873
- Nedd4-1 is an exceptional prognostic biomarker for GCA. PMID: 25395181
- Tyrosine phosphorylation of NEDD4 activates its ubiquitin ligase activity. PMID: 25292214
- NEDD4-1 overexpression sensitizes cancer cells to etoposide-induced apoptosis by reducing SAG levels through targeted degradation. SAG is added to a growing list of NEDD4-1 substrates and mediates its biological function. PMID: 25216516
- Data show that NEDD4-1 E3 ligase as a novel component of the tumor suppressor protein p53/proto-oncogene proteins c-mdm2 regulatory feedback loop that controls p53 activity during stress responses. PMID: 24413081
- Results demonstrated that NEDD4 is a novel interaction partner of HER3 and the C-terminal tail of HER3 interacts with the WW domains of NEDD4. PMID: 24662824
- NEDD4 has been shown to play a critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumour suppressor PTEN. [Review] PMID: 25527121
- Findings suggest that the NEDD4-PTEN pathway is dysregulated in myotonic dystrophy type 2 skeletal muscle. PMID: 24907641
- Findings revealed the CKI/SCF(beta-TRCP) signaling axis as the upstream negative regulator of NEDD4. PMID: 24657926
- Relevant interaction partners of GUCD1 were identified, direct interactions with NEDD4-1 (E3 ubiquitin protein ligase neural precursor cell expressed, developmentally downregulated gene 4) was revealed, the latest resulted in control of GUCD1 stability. PMID: 24743017
- Nedd4-1 as a general E3 ubiquitin ligase controls the abundance of all the three major forms of Ras, revealing a fundamental role of Nedd4-1 in regulating Ras signaling. PMID: 24746824
- Lys-63 ubiquitination by Nedd4-1 facilitates endosomal targeting of alpha-synuclein. PMID: 24831002
- NEDD4-1 regulates cell migration and invasion through ubiquitination of CNrasGEF in vitro. PMID: 24340059
- NEDD4 directly interacts with LATS1, leading to ubiquitination and decreased levels of LATS1 and, thus, increased YAP localization in the nucleus, which subsequently increases the transcriptional activity of YAP. PMID: 24107629
顯示更多
收起更多
-
亞細(xì)胞定位：Cytoplasm. Cell membrane; Peripheral membrane protein.
-
數(shù)據(jù)庫鏈接：
Most popular with customers
-
Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human R-spondin-1 (RSPO1), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human C5a anaphylatoxin chemotactic receptor 1 (C5AR1)-VLPs (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Express system: Mammalian cell
Species: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
-
Recombinant Human Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Interleukin-2 (IL2) (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Myosin regulatory light chain 12B (MYL12B) (Active)
Express system: E.coli
Species: Homo sapiens (Human)
-
Recombinant Macaca fascicularis C-type lectin domain family 4 member C(CLEC4C), partial (Active)
Express system: Mammalian cell
Species: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
