Recombinant Human 5'-3' exoribonuclease 2 (XRN2), partial

中文名稱：

人XRN2重組蛋白
貨號(hào)：

CSB-YP863940HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人XRN2重組蛋白
貨號(hào)：

CSB-EP863940HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人XRN2重組蛋白
貨號(hào)：

CSB-EP863940HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人XRN2重組蛋白
貨號(hào)：

CSB-BP863940HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人XRN2重組蛋白
貨號(hào)：

CSB-MP863940HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

XRN2
Uniprot No.：

Q9H0D6
別名：

XRN2; 5'-3' exoribonuclease 2; EC 3.1.13.-; DHM1-like protein; DHP protein
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites.
基因功能參考文獻(xiàn)：
1. Xrn2 depletion induces a transcriptional termination defect at the majority of protein-coding genes. PMID: 30035655
2. depletion of NKRF, XRN2 or DHX15 impairs an early pre-rRNA cleavage step. PMID: 28115624
3. XRN2 is a novel regulator of EMT that contributes to the metastatic processes in lung cancer through a novel miRNA regulatory mechanism. PMID: 28319071
4. XRN2 has a role in R-loop resolution, suppression of replication stress, and maintenance of genomic stability PMID: 27437695
5. Chromatin immunoprecipitation experiments further show that IL-1 stimulation leads to decrease in NKRF aa 421-429 phosphorylation and dissociation of NELF-E and XRN2 by concomitant resumption of transcription elongation of a synthetic reporter PMID: 26340924
6. P-TEFb regulates termination by promoting chromatin recruitment and activation of a cotranscriptional RNA processing enzyme, Xrn2. PMID: 26728557
7. The results suggest that CARF regulates early steps of pre-rRNA processing during ribosome biogenesis by controlling spatial distribution of XRN2 between the nucleoplasm and nucleolus. PMID: 26531822
8. Kinetic competition between elongating pol II and the Xrn2 exonuclease is integral to termination of transcription on most human genes. PMID: 26474067
9. found that the 3' fragments of target pre-mRNA generated by ASO were almost completely degraded from their 5' ends by nuclear XRN2 after RNase H1-mediated cleavage PMID: 26159921
10. The restriction of JFH1 and H77D hepatitis C virus replication by Xrn2 is likely indirect in nature and possibly linked to cytopathic effects of these robustly replicating viruses. PMID: 25673723
11. Xrn2 has a cytoplasmic, antiviral function against HCV that is counteracted by HCV's subversion of miR-122 to form a protective oligomeric complex at the 5' end of the viral genome. PMID: 25121753
12. Mammalian target of rapamycin protein regulates the diffusion of Xrn2 from the nucleolus to the nucleoplasm under heat stress conditions. PMID: 25128458
13. hnRNPK may play a role in recruitment of XRN2 to gene loci thus regulating coupling 3'-end pre-mRNA processing to transcription termination. PMID: 23857582
14. Microprocessor orchestrates the recruitment of termination factors Setx and Xrn2, and the 3'-5' exoribonuclease, Rrp6, to initiate RNAPII pausing and premature termination at the HIV-1 promoter through cleavage of the stem-loop RNA, TAR. PMID: 22980978
15. Xrn2 associated with and co-transcriptionally degraded nascent beta-globin transcripts. Evidence was provided that many endogenous pre-mRNAs are also co-transcriptionally degraded by Xrn2 when their processing is inhibited by Spliceostatin A. PMID: 22522706
16. Xrn2 has a role in control of transcription by RNA polymerase II. PMID: 22483619
17. The results predict that senataxin is required for the resolution of R-loops formed downstream of the poly(A) signal, allowing Xrn2 recruitment and efficient Pol II transcriptional termination. PMID: 21700224
18. Genetic variants regulating Xrn2 expression in cis are determinants of spontaneous lung tumor susceptibility in mice and have genetic equivalents in lung cancer susceptibility in human beings. PMID: 19915612
19. co-transcriptional cleavage acts as a precursor to termination by presenting a free RNA 5' end that is recognized by the human 5' --> 3' exonuclease Xrn2 PMID: 15565158
20. XRN2b is mainly expressed in blood leukocyte tissue, while XRN2a is detected in several human tissues and in human tumor tissues PMID: 16147866
21. p54 is present along the length of genes, and small interfering RNA (siRNA)-mediated knockdown leads to defects in XRN2 recruitment and termination. PMID: 17639083
22. Degradation of the downstream cleavage product (DCP) does not depend on the Xrn2 5' exonuclease, suggesting that CPSF-73 degrades the DCP both in vitro and in vivo. PMID: 18955505
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亞細(xì)胞定位：

Nucleus, nucleolus.
蛋白家族：

5'-3' exonuclease family, XRN2/RAT1 subfamily
組織特異性：

Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.
數(shù)據(jù)庫鏈接：

HGNC: 12836

OMIM: 608851

KEGG: hsa:22803

STRING: 9606.ENSP00000366396

UniGene: Hs.255932