Recombinant Escherichia coli mRNA interferase YafQ (yafQ)

中文名稱：

大腸桿菌yafQ重組蛋白
貨號：

CSB-YP675091ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌yafQ重組蛋白
貨號：

CSB-EP675091ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌yafQ重組蛋白
貨號：

CSB-EP675091ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌yafQ重組蛋白
貨號：

CSB-BP675091ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌yafQ重組蛋白
貨號：

CSB-MP675091ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

yafQ
Uniprot No.：

Q47149
別名：

yafQ; b0225; JW0215; mRNA interferase toxin YafQ; EC 3.1.-.-; Endoribonuclease YafQ; Toxin YafQ
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-92
氨基酸序列

MIQRDIEYSG QYSKDVKLAQ KRHKDMNKLK YLMTLLINNT LPLPAVYKDH PLQGSWKGYR DAHVEPDWIL IYKLTDKLLR FERTGTHAAL FG
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Toxic component of a type II toxin-antitoxin (TA) system. A sequence-specific mRNA endoribonuclease that inhibits translation elongation and induces bacterial stasis. Cleavage occurs between the second and third residue of the Lys codon followed by a G or A (5'AAA(G/A)3'), is reading-frame dependent and occurs within the 5' end of most mRNAs. Ribosome-binding confers the sequence specificity and reading frame-dependence. When overexpressed in liquid media YafQ partially inhibits protein synthesis, with a reduction in growth rate and colony growth rate. This effect is counteracted by coexpression with cognate antitoxin DinJ. YafQ and DinJ together bind their own promoter, and repress its expression.; Cell death governed by the MazE-MazF and DinJ-YafQ TA systems seems to play a role in biofilm formation.
基因功能參考文獻：
1. The findings demonstrate the plasticity of ribosome-dependent toxin active site residues by the E. coli YafQ toxin and further our understanding of which toxin residues are important for function. PMID: 26261214
2. YafQ increases persistence by reducing indole, and toxin/antitoxin systems are related to cell signalling. PMID: 25041421
3. The endoribonuclease activity of YafQ was inhibited in the complex with DinJ antitoxin in vitro. PMID: 22247505
4. Acts as toxin in active toxin-antitoxin module with DinJ. Structure modelling suggests that it might act as a sequence-specific mRNA endoribonuclease. PMID: 17263853
5. YafQ endoribonuclease expression resulted in translation, but not transcription or replication defects. PMID: 19210620
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蛋白家族：

RelE toxin family, YafQ subfamily
數據庫鏈接：

KEGG: ecj:JW0215

STRING: 316385.ECDH10B_0207