Recombinant Escherichia coli mRNA interferase YafO (yafO)

中文名稱：

大腸桿菌yafO重組蛋白
貨號：

CSB-YP672851ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌yafO重組蛋白
貨號：

CSB-EP672851ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌yafO重組蛋白
貨號：

CSB-EP672851ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌yafO重組蛋白
貨號：

CSB-BP672851ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌yafO重組蛋白
貨號：

CSB-MP672851ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

yafO
Uniprot No.：

Q47157
別名：

yafO; b0233; JW0223; mRNA interferase toxin YafO; EC 3.1.-.-; Endoribonuclease YafO; Toxin YafO
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-132
氨基酸序列

MRVFKTKLIR LQLTAEELDA LTADFISYKR DGVLPDIFGR DALYDDSFTW PLIKFERVAH IHLANENNPF PPQLRQFSRT NDEAHLVYCQ GAFDEQAWLL IAILKPEPHK LARDNNQMHK IGKMAEAFRM RF
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Toxic component of a type II toxin-antitoxin (TA) system. A translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin YafN. Overexpression causes cleavage of a number of mRNAs in a ribosome-dependent fashion. YafO binding to the 50S ribosomal subunit in the translation complex induces mRNA cleavage 3' to the region protected by the ribosome; YafO alone is not able to digest mRNA.
數據庫鏈接：

KEGG: ecj:JW0223

STRING: 316385.ECDH10B_0215