fimA; pilA; b4314; JW4277Type-1 fimbrial protein; A chain; Type-1A pilin
種屬:
Escherichia coli (strain K12)
蛋白長度:
Full Length of Mature Protein
來源:
E.coli
分子量:
31.8kDa
表達區(qū)域:
24-182aa
氨基酸序列
AATTVNGGTVHFKGEVVNAACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAIDAGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSSETTLNNGTNTIPFQARYFATGAATPGAANADATFKVQYQ Note: The complete sequence may
include tag sequence, target protein sequence, linker sequence
and extra sequence that is translated with the protein sequence
for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant
protein is critical to your application, please explicitly
request the full and complete sequence of this protein before
ordering.
蛋白標簽:
N-terminal 6xHis-SUMO-tagged
產品提供形式:
Liquid or
Lyophilized powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
緩沖液:
If the delivery form is liquid, the default storage buffer is
Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have
any special requirement for the glycerol content, please remark
when you place the order. If the delivery form is lyophilized powder, the buffer before
lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
3-7 business days
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
基因功能參考文獻:
FimA is capable of intramolecular self-complementation via its own donor strand, as evidenced by the loss of folding competence upon donor strand deletion. PMID: 21816158
Results show that pilA recombinant protein has some immunoprotection effect with the challenging of virulent strains of E. coli GH1.2. PMID: 17577990
report the identification of a soluble form of the pilus protein FimA from the culture supernatants of E. coli K1, Salmonella, and Shigella that can potently inhibit Bax-mediated release of cytochrome c from isolated mitochondria. PMID: 20347420