Recombinant Escherichia coli Outer-membrane lipoprotein carrier protein (lolA)

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中文名稱：

大腸桿菌O9：H4lolA重組蛋白
貨號：

CSB-YP419575EJF
說明書：

MSDS Datasheet
規格：

￥2616
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP419575EJF could indicate that this peptide derived from Yeast-expressed Escherichia coli O9:H4 (strain HS) lolA.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP419575EJF could indicate that this peptide derived from Yeast-expressed Escherichia coli O9:H4 (strain HS) lolA.
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產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

lolA
Uniprot No.：

A7ZYJ5
別名：

lolA; EcHS_A0996; Outer-membrane lipoprotein carrier protein
種屬：

Escherichia coli O9:H4 (strain HS)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

22.3 kDa
表達區域：

22-203aa
氨基酸序列

DAASDLKSRLDKVSSFHASFTQKVTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWLKDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQFSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答

I have some questions about CSB-EP419575EJF Recombinant Escherichia coli O9:H4 (strain HS) Outer-membrane lipoprotein carrier protein.
1.How long does it take to deliver 1mg of protein?
2.What is the concentration of the protein?
3.Do you do any activity check?
4.Could you please provide me with the full sequence including his-tag and potential cleavage sites.

Very nice to receive your inquiry.
1. The lead time for 1mg is 10-15 working days
2. The specific concentration will be determined after production and shown in COA. If customer has special requirement for concentration, please kindly confirm with us in pre-sales and remark in PO.
3. We haven't validated the activity of this protein. This protein is derived from Escherichia coli, which is full length of mature protein expressed in prokaryotic expression system.
Its expression form is supernatant and can maintain its activity in theory, but we haven't performed validation yet.
4. The following is the complete sequence including tag sequence and target protein sequence.

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFRT+DAASDLKSRLDKVSSFHASFTQKVTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWLKDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQFSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK

The sequence in front of "+" is the tag sequence, and the sequence behind of "+" is the target protein sequence.

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靶點詳情

功能：

Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane).
亞細胞定位：

Periplasm.
蛋白家族：

LolA family
數據庫鏈接：

KEGG: ecx:EcHS_A0996