Recombinant Escherichia coli Molecular chaperone Hsp31 and glyoxalase 3 (hchA)

中文名稱：

大腸桿菌hchA重組蛋白
貨號：

CSB-YP341244ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌hchA重組蛋白
貨號：

CSB-EP341244ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌hchA重組蛋白
貨號：

CSB-EP341244ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌hchA重組蛋白
貨號：

CSB-BP341244ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌hchA重組蛋白
貨號：

CSB-MP341244ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

hchA
Uniprot No.：

P31658
別名：

hchA; yedU; yzzC; b1967; JW1950; Protein/nucleic acid deglycase 1; EC 3.1.2.-; EC 3.5.1.-; EC 3.5.1.124; Glyoxalase III; EC 4.2.1.130; Holding molecular chaperone; Hsp31; Maillard deglycase
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-283
氨基酸序列

TVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFS TGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIG LPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGR VHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Is able to repair glycated serum albumin, aspartate aminotransferase, glyceraldehyde-3-phosphate dehydrogenase, and fructose biphosphate aldolase. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE) that cause irreversible damage. Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Has been reported to display chaperone, peptidase and glutathione-independent glyoxalase activities. However, these apparently disparate activities are all recruited to execute its protein deglycase primary function. Plays an important role in protecting cells from carbonyl stress, severe heat shock and starvation, as well as in acid resistance of stationary-phase cells.
基因功能參考文獻：
1. The authors found that Hsp31 displays glyoxalase activity that catalyses the conversion of methylglyoxal (MG) to d-lactate without an additional cofactor. PMID: 21696459
2. Hsp31, the hchA gene product, plays an important role in the acid resistance of starved Escherichia coli but that it has little influence on oxidative-stress survival PMID: 17158627
3. Study presents evidence for an additional, novel, temperature-dependent control of Hsp31 expression by increased transcript stability. PMID: 17526696
亞細胞定位：

Cytoplasm.
蛋白家族：

Peptidase C56 family, HchA subfamily
數據庫鏈接：

KEGG: ecj:JW1950

STRING: 316385.ECDH10B_2110