Recombinant Escherichia coli Hydrogenase isoenzymes formation protein hypD (hypD)

中文名稱：

大腸桿菌hypD重組蛋白
貨號：

CSB-YP326123ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌hypD重組蛋白
貨號：

CSB-EP326123ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌hypD重組蛋白
貨號：

CSB-EP326123ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌hypD重組蛋白
貨號：

CSB-BP326123ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌hypD重組蛋白
貨號：

CSB-MP326123ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

hypD
Uniprot No.：

P24192
別名：

hypD; b2729; JW2699; Hydrogenase maturation factor HypD; Hydrogenase isoenzymes formation protein HypD
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-373
氨基酸序列

MRFVDEYRAP EQVMQLIEHL RERASHLSYT AERPLRIMEV CGGHTHAIFK FGLDQLLPEN VEFIHGPGCP VCVLPMGRID TCVEIASHPE VIFCTFGDAM RVPGKQGSLL QAKARGADVR IVYSPMDALK LAQENPTRKV VFFGLGFETT MPTTAITLQQ AKARDVQNFY FFCQHITLIP TLRSLLEQPD NGIDAFLAPG HVSMVIGTDA YNFIASDFHR PLVVAGFEPL DLLQGVVMLV QQKIAAHSKV ENQYRRVVPD AGNLLAQQAI ADVFCVNGDS EWRGLGVIES SGVHLTPDYQ RFDAEAHFRP APQQVCDDPR ARCGEVLTGK CKPHQCPLFG NTCNPQTAFG ALMVSSEGAC AAWYQYRQQE SEA
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in the maturation of [NiFe] hydrogenases. Involved in the biosynthesis of the Fe(CN)(2)CO cofactor. HypD may act as a scaffold on which the Fe(CN)(2)CO cofactor is formed. In complex with HypC, accepts the cyanide ligand generated by HypF and HypE, and also coordinates the carbon monoxide ligand. Required for the formation of all three hydrogenase isoenzymes (Probable).
基因功能參考文獻：
1. analysis of a cell-free approach for H-cluster synthesis and hydrogenase maturation by using separately expressed and purified HydE, HydF, and HydG PMID: 23049878
2. dynamic complex between the hydrogenase maturation proteins HypD, HypC, HypE and HypF, which is the site of ligand biosynthesis and attachment to the iron atom of the NiFe site in hydrogenase PMID: 15504408
蛋白家族：

HypD family
數據庫鏈接：

KEGG: ecj:JW2699

STRING: 316385.ECDH10B_2897