Recombinant Escherichia coli Cytidylate kinase (cmk)

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文獻引用 (1)

中文名稱：

Recombinant Escherichia coli Cytidylate kinase(cmk)
貨號：

CSB-EP363865ENV
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

cmk
Uniprot No.：

P0A6I0
別名：

cmk; mssA; ycaF; ycaG; b0910; JW0893; Cytidylate kinase; CK; EC 2.7.4.25; Cytidine monophosphate kinase; CMP kinase; Protein MssA; p25
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length
來源：

E.coli
分子量：

40.7kDa
表達區域：

1-227aa
氨基酸序列

MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLALAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQVAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRMLQLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQYARQKLALA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The production of this Recombinant E.coli cmk protein started with the cmk gene synthesis. And then using recombinant DNA technology, the cmk gene was inserted into an expression vector so that we could get the recombinant express plasmid of cmk. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant E.coli cmk protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 90%+ determined by SDS-PAGE.
cmk (also called mssA, ycaF or ycaG) is a gene providing an instruction of making a protein named cytidylate kinase (CK) in Escherichia coli. The protein encoded by this gene is also known as cytidine monophosphate kinase (CMP kinase), protein MssA or p25. This protein is specific for CMP and dCMP and catalyses reversible transfer of the γ-phosphoryl group from a nucleoside triphosphate, generally ATP. It is involved in nucleobase-containing small molecule interconversion and pyrimidine nucleotide metabolic process.
Datasheet & COA：

Please contact us to get it.

引用文獻

Ubiquitin: Characterization of a Host Cell Protein Covalently Attached to a Monoclonal Antibody Product by LC-MS/MS R Kufer,Journal of pharmaceutical sciences,2023

產品評價

靶點詳情

功能：

ATP, dATP, and GTP are equally effective as phosphate donors. CMP and dCMP are the best phosphate acceptors.
基因功能參考文獻：
1. analysis of of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase PMID: 17542990
2. Data show a molecular dynamics simulation on the CMK dimer complex, and protein-protein interactions. PMID: 19198899
亞細胞定位：

Cytoplasm.
蛋白家族：

Cytidylate kinase family, Type 1 subfamily
數據庫鏈接：

KEGG: ecj:JW0893

STRING: 316385.ECDH10B_0980