Recombinant Escherichia coli Chromosome partition protein mukF (mukF)

中文名稱：

大腸桿菌mukF重組蛋白
貨號：

CSB-YP351500ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌mukF重組蛋白
貨號：

CSB-EP351500ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌mukF重組蛋白
貨號：

CSB-EP351500ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌mukF重組蛋白
貨號：

CSB-BP351500ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌mukF重組蛋白
貨號：

CSB-MP351500ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

mukF
Uniprot No.：

P60293
別名：

mukF; kicB; b0922; JW0905Chromosome partition protein MukF; Protein KicB
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-440
氨基酸序列

MSEFSQTVPE LVAWARKNDF SISLPVDRLS FLLAVATLNG ERLDGEMSEG ELVDAFRHVS DAFEQTSETI GVRANNAIND MVRQRLLNRF TSEQAEGNAI YRLTPLGIGI TDYYIRQREF STLRLSMQLS IVAGELKRAA DAAEEGGDEF HWHRNVYAPL KYSVAEIFDS IDLTQRLMDE QQQQVKDDIA QLLNKDWRAA ISSCELLLSE TSGTLRELQD TLEAAGDKLQ ANLLRIQDAT MTHDDLHFVD RLVFDLQSKL DRIISWGQQS IDLWIGYDRH VHKFIRTAID MDKNRVFAQR LRQSVQTYFD EPWALTYANA DRLLDMRDEE MALRDEEVTG ELPEDLEYEE FNEIREQLAA IIEEQLAVYK TRQVPLDLGL VVREYLSQYP RARHFDVARI VIDQAVRLGV AQADFTGLPA KWQPINDYGA KVQAHVIDKY
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.
基因功能參考文獻：
1. activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together. PMID: 27697840
2. MatP regulates the action of topoisomerase IV and MukBEF in chromosome segregation. PMID: 26818444
3. Taken together, the data implicate MukBEF as a key component of the DNA segregation process by acting in concert with TopoIV to promote decatenation and positioning of newly replicated oris. PMID: 24520061
4. MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors. PMID: 23112333
5. findings show here that MukBEF generates and maintains the cellular positioning of chromosome loci independently of DNA replication. PMID: 22753058
6. Competing MukB-MukE and MukE-MukE interactions may regulate the formation of higher-order structures of bacterial condensin. PMID: 21855551
7. The crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution shows that this region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. PMID: 15902272
8. MukEF Is required for stable association of MukB with the chromosome. PMID: 17644586
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亞細胞定位：

Cytoplasm, nucleoid. Note=Restricted to the nucleoid region.
蛋白家族：

MukF family
數據庫鏈接：

KEGG: ecj:JW0905

STRING: 316385.ECDH10B_0992