Recombinant Drosophila melanogaster Poly (A) RNA polymerase gld-2 homolog B (wisp), partial

中文名稱：

黑腹果蠅wisp重組蛋白
貨號：

CSB-YP895214DLU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

黑腹果蠅wisp重組蛋白
貨號：

CSB-EP895214DLU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

黑腹果蠅wisp重組蛋白
貨號：

CSB-EP895214DLU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

黑腹果蠅wisp重組蛋白
貨號：

CSB-BP895214DLU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

黑腹果蠅wisp重組蛋白
貨號：

CSB-MP895214DLU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

wisp
Uniprot No.：

Q9VYS4
別名：

wisp; CG15737; Poly(A) RNA polymerase gld-2 homolog B; EC 2.7.7.19; Protein wispy
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific maternal RNAs (bcd, Tl, and tor), forming a poly(A) tail, during late oogenesis and early embryogenesis. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Required for localization of mRNAs to both poles of the egg, to recruit or maintain known centrosomal proteins with two types of microtubule organizing centers (MTOCs): the central MTOC that forms between the meiosis II tandem spindles and the centrosomes of the mitotic spindle. Required at the final stage of oogenesis for meiosis I metaphase arrest and for progression beyond this stage.
基因功能參考文獻：
1. piRNAs and Aub actively protect germ cell mRNAs in the germ plasm. Aub directly interacts with the germline-specific poly(A) polymerase Wispy, thus leading to mRNA polyadenylation and stabilization in the germ plasm. PMID: 29101389
2. Localized cytoplasmic polyadenylation of grk mRNA by Wisp and Orb is necessary to achieve appropriate Grk protein accumulation in the D/A corner of the oocyte during mid to late oogenesis. PMID: 26214278
3. Results suggest that Wisp interacts with Ago1 and induces miRNA adenylation and facilitates its downregulation. PMID: 25454948
4. Wispy regulates polyadenylation of many mRNAs in late-stage oocytes. PMID: 23978535
亞細胞定位：

Cytoplasm.
蛋白家族：

DNA polymerase type-B-like family, GLD2 subfamily
組織特異性：

Expressed in ovaries. Not expressed in adult males.
數據庫鏈接：

KEGG: dme:Dmel_CG15737

STRING: 7227.FBpp0073417

UniGene: Dm.6637