Recombinant Clostridium perfringens Perfringolysin O (pfo)

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文獻引用 (5)

中文名稱：

產氣莢膜梭狀芽胞桿菌pfo重組蛋白
貨號：

CSB-EP314820CMB
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo.
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

pfo
Uniprot No.：

P0C2E9
別名：

pfo; pfoA; pfoR; CPE0163; Perfringolysin O; Theta-toxin; Thiol-activated cytolysin
種屬：

Clostridium perfringens (strain 13 / Type A)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

68.7kDa
表達區域：

29-500aa
氨基酸序列

KDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKKTGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKGENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVDFNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVKLETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNATFSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGNEVLTHKTWDGNYQDKTAHYSTVIPLEANARNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTTLYPGSSITYN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Recombinant Clostridium perfringens Perfringolysin O (pfo)是一種經重組制備的產氣莢膜梭菌（Clostridium perfringens）溶原性毒素O蛋白。Perfringolysin O是產氣莢膜梭菌的一個主要毒素，它能夠破壞目標細胞的細胞膜，導致細胞死亡。Perfringolysin O的研究對于了解產氣莢膜梭菌感染機制和溶原性毒素的生物學特性具有重要意義。

華美生物的重組pfo蛋白，由大腸桿菌表達系統表達，具有良好的穩定性和純度，可以更好幫助您研究其在細胞毒性和感染過程中的作用機制，以及與宿主細胞相互作用的方式；可以被廣泛應用于生物醫學研究中，如細胞毒性實驗、毒力機制研究和免疫學研究等。
Datasheet & COA：

Please contact us to get it.

引用文獻

Antagonistic nanobodies implicate mechanism of GSDMD pore formation and potential therapeutic application LDJ Schiffelers, YM Tesfamariam, LM Jenster,Nature Communications,2024
Tau induces inflammasome activation and microgliosis through acetylating NLRP3 L Zhang,Clinical and translational medicine,2024
Small cytosolic double-stranded DNA represses cyclic GMP-AMP synthase activation and induces autophagy Y Liu,Cell reports,2023
CD4+ T cell-mediated recognition of a conserved cholesterol-dependent cytolysin epitope generates broad antibacterial immunity L Ciacchi,Immunity,2023
Network analysis of large-scale ImmGen and Tabula Muris datasets highlights metabolic diversity of tissue mononuclear phagocytes A Gainullina,Cell reports,2023

產品評價

靶點詳情

功能：

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
亞細胞定位：

Secreted. Host cell membrane; Multi-pass membrane protein.
蛋白家族：

Thiol-activated cytolysin family
數據庫鏈接：

KEGG: cpe:CPE0163