Recombinant Chlamydomonas reinhardtii Autolysin

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中文名稱：

Recombinant Chlamydomonas reinhardtii Autolysin
貨號：

CSB-EP327029DSJ
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

N/A
Uniprot No.：

P31178
別名：

Autolysin; EC 3.4.24.38; Gamete lytic enzyme; GLE; Gametolysin
種屬：

Chlamydomonas reinhardtii (Chlamydomonas smithii)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

65.6kDa
表達區域：

184-638aa
氨基酸序列

EIYAGKPIDLRTIVYIMDFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERRRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The gene fragment corresponding to amino acid residues 184-638 of the Chlamydomonas reinhardtii autolysin is expressed in E.coli. The resulting protein is fused with the 6xHis-SUMO motif at the N-terminus, leading to the production of the 6xHis-SUMO-tagged recombinant autolysin protein. This fusion protein covers the full length of the mature protein. Its purity is over 90% determined by SDS-PAGE. It presented on the 65 kDa molecular weight band on the gel. It is available now. The target protein autolysin is an endogenous lytic enzyme that degrades the peptidoglycan components of biological cells thus enabling the separation of daughter cells following cell division. It also participates in cell growth, cell wall metabolism, and peptidoglycan turnover.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.
亞細胞定位：

Periplasm. Secreted, cell wall. Note=Stored in the periplasm of gametes until its release. Secreted concurrently with release of the cell walls.
蛋白家族：

Peptidase M11 family