Recombinant Bovine Unconventionnal myosin-X (MYO10), partial

中文名稱：

牛MYO10重組蛋白
貨號：

CSB-YP015331BO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

牛MYO10重組蛋白
貨號：

CSB-EP015331BO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

牛MYO10重組蛋白
貨號：

CSB-EP015331BO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

牛MYO10重組蛋白
貨號：

CSB-BP015331BO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

牛MYO10重組蛋白
貨號：

CSB-MP015331BO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

MYO10
Uniprot No.：

P79114
別名：

MYO10; Unconventional myosin-X; Unconventional myosin-10
種屬：

Bos taurus (Bovine)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate, which are then moved relative to actin filaments. Stimulates the formation and elongation of filopodia. Regulates cell shape, cell spreading and cell adhesion. Plays a role in formation of the podosome belt in osteoclasts.
基因功能參考文獻：
1. A Combination of Diffusion and Active Translocation Localizes Myosin 10 to the Filopodial Tip. PMID: 27566544
2. Single alpha-helices domain of MYO10 unfolds progressively as the length is increased and refolds progressively as the length is reduced. PMID: 25122759
3. At low optical trap loads, we observed staircase-like processive movements of myosin-10 interacting with the actin filament, consisting of up to six approximately 35-nm steps per binding interaction. PMID: 24753602
4. Myosin X is monomeric and the band 4.1-ezrin-radixin-moesin (FERM) and pleckstrin homology (PH) domains bind to the head intramolecularly, forming an inhibited conformation. PMID: 21666676
5. the structural features of the motor and the track that allow myosin X to recognize actin filament bundles PMID: 20858426
6. myosin X is a membrane-associated molecular motor PMID: 15705568
7. kinetic analysis of function of Myosin X as a high duty ratio motor PMID: 15961399
8. the predicted coiled coil of myosin 10 forms a novel elongated structure in which the proximal region is a SAH domain and the distal region is a SAH domain (or has an unknown extended structure) that dimerizes only at its end PMID: 16030012
9. myosin X recognizes the local structural arrangement of filaments in long bundles, providing a mechanism for sorting cargo to distant target sites. PMID: 18599451
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亞細胞定位：

Cytoplasm, cytosol. Cell projection, lamellipodium. Cell projection, ruffle. Cytoplasm, cytoskeleton. Cell projection, filopodium tip. Cytoplasm, cell cortex. Cell projection, filopodium membrane; Peripheral membrane protein. Note=May be in an inactive, monomeric conformation in the cytosol. Detected in cytoplasmic punctae and in cell projections. Colocalizes with actin fibers. Interacts with microtubules. Undergoes forward and rearward movements within filopodia. Interaction with membranes containing phosphatidylinositol 3,4,5-trisphosphate mediates localization at filopodium membranes.
蛋白家族：

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
組織特異性：

Detected in kidney, testis, liver, kidney, cerebellum and brain cortex (at protein level).
數據庫鏈接：

KEGG: bta:281935

STRING: 9913.ENSBTAP00000026812

UniGene: Bt.103339