E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized
powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
基因功能參考文獻:
an extended network of hydrogen bonds connects the agonist retinal binding site to the G protein binding site via conserved amino acid residues. dynamics of the hydrogen-bonding network inside opsin couples to interactions at the G protein binding site PMID: 25138898
instability of the retinitis pigmentosa P23H mutant opsin PMID: 24515108
Ligand binding in opsin apparently follows an induced-fit three-point interaction model, with the ring, 9-methyl group, and aldehyde group as the determining selection elements. PMID: 15544351
the crystal structure of ligand-free native opsin from bovine retinal rod cells at 2.9 angstrom (A) resolution PMID: 18563085
3.2 A crystal structure of the bovine Ops*-GalphaCT peptide complex PMID: 18818650
Mammalian rod opsin biogenesis does not appear to have an absolute requirement for Cnx. PMID: 19116670
a single dibasic degron motif is sufficient to initiate both the ER retention and subsequent degradation of ospin via an endoplasmic reticulum associated degradation pathway PMID: 19878048