Recombinant Bothrops atrox Thrombin-like enzyme batroxobin

中文名稱：

Recombinant Bothrops atrox Thrombin-like enzyme batroxobin
貨號：

CSB-YP356422BUU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Bothrops atrox Thrombin-like enzyme batroxobin
貨號：

CSB-EP356422BUU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Bothrops atrox Thrombin-like enzyme batroxobin
貨號：

CSB-BP356422BUU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Bothrops atrox Thrombin-like enzyme batroxobin
貨號：

CSB-MP356422BUU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
問答及客戶評論
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

N/A
Uniprot No.：

P04971
別名：

; Thrombin-like enzyme batroxobin; BX; SVTLE; EC 3.4.21.74; Bothrops atrox serine proteinase; Defibrase; Fibrinogen-clotting enzyme; Reptilase; Snake venom serine protease; SVSP; Venombin A
種屬：

Bothrops atrox (Barba amarilla) (Fer-de-lance)
蛋白長度：

Full Length of Mature Protein
表達區域：

25-255
氨基酸序列

VIGGDE CDINEHPFLA FMYYSPRYFC GMTLINQEWV LTAAHCNRRF MRIHLGKHAG SVANYDEVVR YPKEKFICPN KKKNVITDKD IMLIRLDRPV KNSEHIAPLS LPSNPPSVGS VCRIMGWGAI TTSEDTYPDV PHCANINLFN NTVCREAYNG LPAKTLCAGV LQGGIDTCGG DSGGPLICNG QFQGILSWGS DPCAEPRKPA FYTKVFDYLP WIQSIIAGNK TATCP
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答

Does Cusabio provide recombinant batroxobin with validated biological activity?

We recommend this protein.
Recombinant Bothrops atrox Thrombin-like enzyme batroxobin
CSB-YP356422BUU >> Yeast Lead time: 35-45 business days.
CSB-EP356422BUU >> E.coli Lead time: 35-45 business days.
CSB-BP356422BUU >> Baculovirus Lead time: 35-50 business days.
CSB-MP356422BUU >> Mammalian cell Lead time: 35-45 business days.
Expression region: 25-255aa ; full length of mature protein;
Tag information: Tag type will be determined during the manufacturing process. (Please let us know if you have any special request of tag, we will preferentially try your desired tag. If the requested tag fails, we will provide other tagged protein. )
Sequence of target protein (Sequence of tag is not included):

VIGGDECDINEHPFLAFMYYSPRYFCGMTLINQEWVLTAAHCNRRFMRIHLGKHAGSVANYDEVVRYPKEKFICPNKKKNVITDKDIMLIRLDRPVKNSEHIAPLSLPSNPPSVGSVCRIMGWGAITTSEDTYPDVPHCANINLFNNTVCREAYNGLPAKTLCAGVLQGGIDTCGGDSGGPLICNGQFQGILSWGSDPCAEPRKPAFYTKVFDYLPWIQSIIAGNKTATCP

Regarding activity
This is a semi-customized protein, we haven't tested activity of this protein, so we can't 100% guaranteed its activity. But what we provide is the full length of the mature protein, which is purified under mild condition, it should be active in theory.
Reference: https://eurekamag.com/pdf/004/004169771.pdf
It reports the cDNA encoding batroxobin was cloned, expressed in Pichia pastoris and the molecular function of purified recombinant protein was also characterized.The purified recombinant protein strongly converted fibrinogen into fibrin clot in vitro, and shortened bleeding time and whole blood coagulation time in vivo. We recommend you to order a small size to have a try.

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靶點詳情

功能：

Thrombin-like snake venom serine protease. Cleaves Arg-Gly bonds in fibrinogen alpha chains (FGA).
亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family, Snake venom subfamily
組織特異性：

Expressed by the venom gland.
數據庫鏈接：

KEGG: ag:AAA48552