Recombinant Bacillus subtilis UPF0079 ATP-binding protein ydiB (ydiB)

中文名稱：

枯草芽孢桿菌tsaE重組蛋白
貨號：

CSB-YP520837BRJ
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

枯草芽孢桿菌tsaE重組蛋白
貨號：

CSB-EP520837BRJ
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

枯草芽孢桿菌tsaE重組蛋白
貨號：

CSB-EP520837BRJ-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

枯草芽孢桿菌tsaE重組蛋白
貨號：

CSB-BP520837BRJ
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

枯草芽孢桿菌tsaE重組蛋白
貨號：

CSB-MP520837BRJ
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

tsaE
Uniprot No.：

O05515
別名：

tsaE; ydiB; BSU05910; tRNA threonylcarbamoyladenosine biosynthesis protein TsaE; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
種屬：

Bacillus subtilis (strain 168)
蛋白長度：

full length protein
表達區域：

1-158
氨基酸序列

MKQLKWRTVN PEETKAIAKL TAAFAKPGDV LTLEGDLGAG KTTFTKGFAE GLGITRIVNS PTFTIIKEYN DGVLPLYHMD VYRMEDESED LGLDEYFHGQ GVCLVEWAHL IEEQLPQERL QIVIKRAGDD EREITFTAVG NRYEMLCEEL SRHDNISN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB; this reaction does not require ATP in vitro. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro, which is modulated by the oligomeric status of the protein.
基因功能參考文獻：
1. A prototypic member of this family, YdiB from Bacillus subtilis, is able to autophosphorylate and to phosphorylate a surrogate substrate, the myelin basic protein. PMID: 28890133
2. YdiB exhibited a low, but significant, ATPase activity, and oligomerization of YdiB affected its ATPase activity. PMID: 19246765
亞細胞定位：

Cytoplasm. Note=Localized predominantly at the cell poles and at the periphery of the bacterium.
蛋白家族：

TsaE family
數據庫鏈接：

KEGG: bsu:BSU05910

STRING: 224308.Bsubs1_010100003313