Recombinant Bacillus subtilis RNA polymerase sigma factor sigW (sigW)
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中文名稱:枯草芽孢桿菌sigW重組蛋白
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貨號:CSB-EP676045BRJ-B
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說明書:
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規(guī)格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:sigW
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Uniprot No.:
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別名:sigW; ybbL; BSU01730; ECF RNA polymerase sigma factor SigW; ECF sigma factor SigW; Alternative RNA polymerase sigma factor SigW; RNA polymerase sigma-W factor; Sigma-W factor
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種屬:Bacillus subtilis (strain 168)
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蛋白長度:full length protein
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表達(dá)區(qū)域:1-187
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氨基酸序列MEMMIKKRIK QVKKGDQDAF ADIVDIYKDK IYQLCYRMLG NVHEAEDIAQ EAFIRAYVNI DSFDINRKFS TWLYRIATNL TIDRIRKKKP DYYLDAEVAG TEGLTMYSQI VADGVLPEDA VVSLELSNTI QQKILKLPDK YRTVIVLKYI DELSLIEIGE ILNIPVGTVK TRIHRGREAL RKQLRDL
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蛋白標(biāo)簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點(diǎn)詳情
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功能:Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Sigma-W controls genes involved in response to cell envelope stress such as antimicrobial peptides, alkaline pH, transport processes and detoxification.
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基因功能參考文獻(xiàn):
- Study determined the first structure of a sigma/Zinc-containing anti-sigma factor complex in the oxidized state. The crystal structures of the SigW/RsiW(cytoplasmic domain) complex in the reduction/zinc binding and oxidation states explain the zinc coordination mode of RsiW, as well as the inhibition mode of SigW activity by RsiW. PMID: 28319136
- controls an antibiosis regulon important in the microbial ecology of soil bacteria. PMID: 16629676
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蛋白家族:Sigma-70 factor family, ECF subfamily
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數(shù)據(jù)庫鏈接:
KEGG: bsu:BSU01730
STRING: 224308.Bsubs1_010100000988
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