Recombinant Arabidopsis thaliana Two pore calcium channel protein 1 (TPC1), partial

中文名稱：

擬南芥TPC1重組蛋白
貨號：

CSB-YP853237DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

擬南芥TPC1重組蛋白
貨號：

CSB-EP853237DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

擬南芥TPC1重組蛋白
貨號：

CSB-EP853237DOA-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

擬南芥TPC1重組蛋白
貨號：

CSB-BP853237DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

擬南芥TPC1重組蛋白
貨號：

CSB-MP853237DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

TPC1
Uniprot No.：

Q94KI8
別名：

TPC1; CCH1; FOU2; At4g03560; F9H3.19; T5L23.5; Two pore calcium channel protein 1; Calcium channel protein 1; AtCCH1; Fatty acid oxygenation up-regulated protein 2; Voltage-dependent calcium channel protein TPC1; AtTPC1
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Functions as a voltage-gated inward-rectifying Ca(2+) channel (VDCC) across the vacuole membrane. Is one of the essential components of the slow vacuolar (SV) channel. Acts as the major ROS-responsive Ca(2+) channel and is the possible target of Al-dependent inhibition. Involved in the regulation of germination and stomatal movement.
基因功能參考文獻(xiàn)：
1. Structural basis for activation of voltage sensor domains in an ion channel TPC1. PMID: 30190435
2. variant (TPC1D454N) depolarizes endomembranes by increasing cation fluxes towards the cytosol PMID: 28885692
3. The ion selectivity of AtTPC1 was determined and compared with the selectivity of human TPC2. TPC1 is selective for Ca(2+) over Na(+), but nonselective among monovalent Li(+), Na(+), and K(+)). AtTPC1 could be converted to a Na(+)-selective channel by mimicking the selectivity filter of HsTPC2. Key residues were identified in the TPC filters that differentiate this selectivity. A Na+-selective mutant was characterized. PMID: 28096396
4. TPC1 role in the systemic response to salt stress PMID: 27261066
5. Structure-function analysis of the Arabidopsis TPC1 channel in planta confirmed that helix S10 operates as the major voltage-sensing site, with Glu450 and Glu478 identified as possible ion-pair partners for voltage-sensing Arg537. PMID: 27270880
6. The domain architecture of TPC1 has been discussed. PMID: 27156118
7. This study discovered that the dimerization of a predicted helix within the carboxyl-terminus is essential for the activity of TPC1. PMID: 26781468
8. crystal structure of TPC1 from Arabidopsis thaliana at 2.87 A resolution as a basis for understanding ion permeation, channel activation, the location of voltage-sensing domains and regulatory ion-binding sites PMID: 26961658
9. crystal structure of a vacuolar two-pore channel from Arabidopsis thaliana, AtTPC1, which functions as a homodimer PMID: 26689363
10. The SV channel fou2 mutation in TCP1 altered sensitivity to luminal Ca2+ levels. PMID: 19298454
11. TPC1 contains a N-terminal dileucine motif (EDPLI) critical for vacuolar targeting PMID: 22490017
12. TPC1 has a role in maintaining differential K and Ca storage across the leaf; it releases Ca2+ from epidermal and bundle sheath cell vacuoles to maintain low [Ca]vac. PMID: 22067997
13. EF-hand 1 modulates the Ca2+-sensitivity of TPC1, whereas EF-hand 2 is essential for the Ca2+-dependent channel opening. PMID: 21736651
14. Results indicate that Glu-450 and Asp-454 of the slow vacuolar channel TPC1 are directly involved in Ca2+ binding, whereas Glu-456 and Glu-457 are probably involved in connecting the luminal Ca2+ binding site to the channel gate. PMID: 21764990
15. AtTPC1 functions in response to external Ca2+ but not to abscisic acid and methyl jasmonate in Arabidopsis guard cells. PMID: 20061305
16. Results suggest that members of TPC1 channel family are reactive oxygen species-responsive calcium channels and are the possible targets of aluminum-dependent inhibition. PMID: 15464979
17. a tpc1 knockout mutant lacks functional slow vacuolar channel activity and is defective in both abscisic acid-induced repression of germination and in the response of stomata to extracellular calcium PMID: 15772667
18. a genetic screen capable of detecting the misregulated activity of 13-lipoxygenase identified a missense mutation in TPC1, indicating that cation fluxes exert control over positive feedback loop for jasmonic acid stimulates its own synthesis. PMID: 17253984
19. results suggest that wild-type TPC1 contributes to their expression of PDF1.2a and THI2 by mechanisms somewhat different from those affecting their expression in fou2 PMID: 17981874
20. study found loss of TPC1 did not change membrane hyperpolarization-activated Ca(2+)-permeable channel activity; we conclude TPC1, under physiological conditions, functions as a vacuolar cation channel without major impact on cytosolic Ca(2+) homeostasis PMID: 18028262
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亞細(xì)胞定位：

Vacuole membrane; Multi-pass membrane protein.
蛋白家族：

Calcium channel alpha-1 subunit (TC 1.A.1.11) family, Two pore calcium channel subfamily
組織特異性：

Ubiquitously expressed.
數(shù)據(jù)庫鏈接：

KEGG: ath:AT4G03560

STRING: 3702.AT4G03560.1

UniGene: At.3957