E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情
功能:
Transcriptional corepressor. May repress the expression of root-promoting genes in the top half of the embryo to allow proper differentiation of the shoot pole during the transition stage of embryogenesis. Regulates the expression of PLT1 and PLT2. Negative regulator of jasmonate responses. Negative regulator of auxin responses. Negative regulator of multiple floral organ identity genes. Required for ovule development.
基因功能參考文獻(xiàn):
relative to mammalian TBL1, evolved a new tetramerization interface and unique and highly conserved surface for interaction with repressors PMID: 28698367
The GIR1 and GIR2 might may link TPL to and participate in epigenetic regulation of root hair development. PMID: 28526412
These data unveil a pivotal role of the co-repressor TPL in the shoot and root meristems. PMID: 28320734
TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1. PMID: 24938363
Topless and Topless-related proteins form a tertiary complex with TIE1 protein at early stages of leaf development. PMID: 23444332
We identified transcription factors interacting with TPL. PMID: 22065421
Arabidopsis JAZ proteins recruit the Groucho/Tup1-type co-repressor TOPLESS (TPL) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA) PMID: 20360743
TPL and other TPR proteins are necessary to repress the expression of root-promoting genes in the top half of the embryo to allow proper differentiation of the shoot pole. [topless][TPL] PMID: 16763149
TPL can interact with IAA12/BODENLOS through an ETHYLENE RESPONSE FACTOR-associated amphiphilic repression motif; study shows TPL is a transcriptional co-repressor & furthers understanding of how auxin regulates transcription during plant development PMID: 18258861
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亞細(xì)胞定位:
Nucleus.
組織特異性:
Expressed in embryo and in extraembryonic tissues. Expressed in inflorescences, flowers, floral meristems, developing anthers and ovules. Detected in the vascular tissues, shoot apical meristem, cotyledons and young leaves. Expressed ubiquitously in the p