E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized
powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產品評價
靶點詳情
基因功能參考文獻:
Data indicate that FRIGIDA protein (FRI) as an activator of EARLY FLOWERING IN SHORT DAYS protein EFS (EFS) and that EFS was a histone H3 Lys36-specific methyltransferase. PMID: 28584923
Structural Analysis of FRIGIDA Flowering-Time Regulator PMID: 26633860
Proteasome-mediated FRI degradation regulates flowering during vernalization in Arabidopsis. PMID: 25538183
Analysis of flowering time data from various Arabidopsis natural accessions indicated that the FRI-Ler allele retains some functionality. PMID: 25207670
The expression of FRI gene in Arabidopsis thaliana plants in extreme conditions in northern margins of species range. PMID: 25474881
The spatial patterns of FRI, FLC, and PHYC polymorphisms are significantly associated with winter temperatures and spring and winter precipitations, respectively. The allelic variation in these genes is involved in climatic adaptation. PMID: 21988878
study presents evidence that the FRI (FRIGIDA) gene exhibits 'adaptive' pleiotropy, producing trait correlations along an axis that results in two adaptive strategies PMID: 23698015
FRI forms a large protein complex which interacts with FLC-specific regulators and recruits cromatin modification factors. PMID: 21282526
FRI and FRL1 contain a stable, central domain that is conserved across the FRI superfamily. PMID: 20405310
The suppression of late flowering is caused by the inability of FRIgida to increase late flowering. PMID: 15361145
The contribution to variation in flowering time and vernalization of the two genes FRIGIDA (FRI) and FLOWERING LOCUS C (FLC), previously shown to be important determinants in natural variation of flowering time is reported. PMID: 15908596
FRI upregulates FLC expression that represses flowering PMID: 16547097
These antagonistic pleiotropic effects reduce the adaptive value of FRI, and helps explain the maintenance of alternative life history strategies across natural populations of A. thaliana. PMID: 17940010
Natural allelic variation in FRI can mediate response to selection for early flowering. PMID: 19317844
FRI up-regulates FLC expression through a cotranscriptional mechanism involving direct physical interaction with the nuclear cap-binding complex with concomitant effects on FLC transcription and splicing. PMID: 19429606