Recombinant Aquifex aeolicus DNA mismatch repair protein mutL (mutL)

中文名稱：

藻類含水層mutL重組蛋白
貨號：

CSB-YP529062DNV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

藻類含水層mutL重組蛋白
貨號：

CSB-EP529062DNV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

藻類含水層mutL重組蛋白
貨號：

CSB-EP529062DNV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

藻類含水層mutL重組蛋白
貨號：

CSB-BP529062DNV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

藻類含水層mutL重組蛋白
貨號：

CSB-MP529062DNV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

mutL
Uniprot No.：

O67518
別名：

mutL; aq_1578; DNA mismatch repair protein MutL
種屬：

Aquifex aeolicus (strain VF5)
蛋白長度：

full length protein
表達區域：

1-425
氨基酸序列

MFVKLLPPEV RKVIAAGEVI ESPVDVVKEL VENSLDAKAT KVEVEIVKGG KRLIRVKDNG TGIHPEDVEK VVLQGATSKI ETEKDLMNIS TYGFRGEALY SISSVSKFKL RSRFFQEKEG KEIEVEAGNI LGTRRVGMPV GTEVEVRDLF FNLPVRRKFL KKEDTERRKV LELIKEYALT NPEVEFTLFS EGRETLKLKK SSLKERVEEV FQTKTEELYA EREGITLRAF VSRNQRQGKY YVFINKRPIQ NKNLKEFLRK VFGYKTLVVL YAELPPFMVD FNVHPKKKEV NILKERKFLE LVRELAGKEK PIVDIPLSQP VKTYKPTYEI LGQMDETFIL VKDSEYLYFV DQHLLEERIN YEKLKDENLA CRISVKAGQK LSEEKIRELI KTWRNLENPH VCPHGRPIYY KIPLREIYEK VGRNY
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.
基因功能參考文獻：
1. The overall structure of the ATPase domain of MutL was comprised of 2 subdomains: the N-terminal ATP-binding subdomain and the C-terminal alpha-beta sandwich subdomain. Site-directed mutagenesis experiment identified DNA-interacting 8 basic AAs distributed across both the 2 subdomains and formed a DNA-binding cleft. The 2 separate DNA-binding sites in the full-length A. aeolicus MutL simultaneously bind a dsDNA molecule. PMID: 28668638
2. The N-terminal domain (NTD) of MutL is monomeric and exhibits a relatively strong DNA-binding activity; it is also clear that NTD promotes the endonuclease activity of carboxyl-terminal domain (CTD). PMID: 20961292
3. A. aeolicus MutL is a human PMS2 homologue that stimulates a PcrA/UvrD/Rep subfamily helicase PMID: 20129926
4. Adenosine triphosphate stimulates Aquifex aeolicus MutL endonuclease activity PMID: 19777055
蛋白家族：

DNA mismatch repair MutL/HexB family
數據庫鏈接：

KEGG: aae:aq_1578

STRING: 224324.aq_1578