SYNCRIP Recombinant Monoclonal Antibody

中文名稱：

SYNCRIP重組抗體
貨號(hào)：

CSB-RA984434A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA984434A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA984434A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela Cells with CSB-RA984434A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
其他：

產(chǎn)品詳情
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產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA984434A0HU SYNCRIP重組單克隆抗體是針對(duì)RNA結(jié)合蛋白SYNCRIP（hnRNP Q）研發(fā)的高特異性檢測(cè)工具。該抗體靶向的SYNCRIP蛋白在RNA代謝過(guò)程中發(fā)揮關(guān)鍵作用，參與mRNA剪接、轉(zhuǎn)運(yùn)及穩(wěn)定性調(diào)控，其功能與神經(jīng)發(fā)育、細(xì)胞應(yīng)激響應(yīng)及病毒復(fù)制機(jī)制密切相關(guān)。本產(chǎn)品經(jīng)過(guò)ELISA、免疫組化（IHC）和免疫熒光（IF）等多平臺(tái)嚴(yán)格驗(yàn)證，在組織樣本和細(xì)胞實(shí)驗(yàn)中均展現(xiàn)出優(yōu)異的特異性和信號(hào)清晰度，推薦使用稀釋度為IHC 1:50-1:200、IF 1:20-1:200。適用于探究RNA調(diào)控機(jī)制、病毒與宿主相互作用、神經(jīng)元分化及腫瘤相關(guān)RNA代謝異常等研究領(lǐng)域，為解析基因表達(dá)調(diào)控網(wǎng)絡(luò)及疾病相關(guān)分子機(jī)制提供可靠工具。本品僅供科學(xué)研究使用，不適用于臨床診斷及其他非科研用途。
Uniprot No.：

O60506
基因名：

SYNCRIP
別名：

Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) (Glycine- and tyrosine-rich RNA-binding protein) (GRY-RBP) (NS1-associated protein 1) (Synaptotagmin-binding, cytoplasmic RNA-interacting protein), SYNCRIP, HNRPQ NSAP1
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human hnRNP Q
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

9C7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; seems not to be essential for GAIT complex function.
基因功能參考文獻(xiàn)：
1. A cryptic RNA-binding domain mediates Syncrip recognition and exosomal partitioning of miRNA targets. PMID: 29483512
2. our data indicate that hnRNP Q1 is a novel trans-acting factor that binds to Aurora-A mRNA 5'-UTRs and regulates its translation, which increases cell proliferation and contributes to tumorigenesis in colorectal cancer PMID: 28079881
3. SYNCRIP is required for survival of leukemia cells. SYNCRIP controls the myeloid leukemia stem cell program. PMID: 28436985
4. The acidic domain is a unique structural feature of the splicing factor SYNCRIP. PMID: 27081926
5. hnRNPQ6 is required for APOBEC1-enhanced IL8 production. PMID: 25100733
6. The hnRNP Q is a novel substrate of SHP2 and the SHP2 activity may be also involved in RNA metabolisms via dephosphorylation of hnRNP Q. PMID: 23946508
7. The inhibitory effect of hnRNP Q on YB-1 mRNA translation can be explained by its ability to pro-mote YB-1 binding to the regulatory element within the YB-1 mRNA 3' UTR. PMID: 23980891
8. These data indicate that hnRNP Q can stimulate the protein production of HIV-1 Rev-dependent mRNAs without changing mRNA levels and mRNA export, respectively. PMID: 23679954
9. Data demonstrate that expression levels of hnRNP A1, Q, K, R, and U influence HIV-1 production by persistently infected PMID: 19808671
10. The overexpression of NSAP1 specifically enhanced HCV IRES-dependent translation, and knockdown of NSAP1 by use of a small interfering RNA specifically inhibited the translation of HCV mRNA. PMID: 15340051
11. SYNCRIP is transported within the dendrite as a component of mRNA granules PMID: 15475564
12. methylation of hnRNPQ is important for its nuclear localization PMID: 16765914
13. Results suggest hnRNPQ interacts via its acidic domain (AcD) with Apobec1 and that this interaction is regulated by AcD phosphorylation. PMID: 17010310
14. Data demonstrate that hnRNP Q is a splicing modulator of SMN2, further underscoring the potential of hnRNP Q as a therapeutic target for spinal muscular atrophy. PMID: 18794368
15. Results demonstrate that galectin-3 stabilizes hnRNP Q via complex formation, and reduction in the hnRNP Q level leads to slow proliferation and less susceptibility to 5-FU. PMID: 19137262
16. SYNCRIP participates in both RNA replication and translation in HCV life cycle. PMID: 19232660
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亞細(xì)胞定位：

Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus.; [Isoform 1]: Nucleus, nucleoplasm.; [Isoform 2]: Nucleus, nucleoplasm.; [Isoform 3]: Nucleus, nucleoplasm.
組織特異性：

Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 16918

KEGG: hsa:10492

STRING: 9606.ENSP00000358635

UniGene: Hs.571177

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