SNAP23 Recombinant Monoclonal Antibody

說明書

中文名稱：

SNAP23重組抗體
貨號：

CSB-RA827145A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA827145A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA827145A0HU是針對SNAP23靶點研發的重組單克隆抗體，適用于ELISA和免疫組化（IHC）實驗。SNAP23作為SNARE蛋白家族成員，在細胞膜融合過程中發揮關鍵作用，廣泛參與囊泡運輸、神經遞質釋放及激素分泌等生物學過程。本抗體通過重組技術制備，具有高特異性和批次間一致性，經實驗驗證可在IHC中實現清晰的組織定位，推薦使用1:50-1:200稀釋比例，能有效識別福爾馬林固定石蠟包埋樣本中的天然靶蛋白，且在多種組織類型中表現出低背景干擾。其ELISA檢測性能經過線性范圍驗證，適用于定量分析樣本中的SNAP23表達水平。該產品為研究胞吐作用調控機制、細胞分泌通路異常相關疾病模型及膜融合動態過程提供可靠工具，可應用于神經生物學、內分泌學及細胞運輸領域的分子機制探索，為體外實驗和病理分析提供精準檢測支持。
Uniprot No.：

O00161
基因名：

SNAP23
別名：

Synaptosomal-associated protein 23 (SNAP-23) (Vesicle-membrane fusion protein SNAP-23), SNAP23
反應種屬：

Human
免疫原：

A synthesized peptide derived from human SNAP23
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2D8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
基因功能參考文獻：
1. PKM2 promotes tumor cell exosome release via phosphorylating protein SNAP23. PMID: 28067230
2. SNAP23 suppressed progression of cervical cancer and induced cell cycle G2/M arrest via upregulating p21(cip1) and downregulating CyclinB1 PMID: 29908998
3. Knockdown of VAMP3 and SNAP23 reduces endothelial secretion of miR-126-3p and miR-200a-3p, as well as the proliferation, migration, and suppression of contractile markers in smooth muscle cells caused by vascular endothelial cell-coculture. PMID: 28716920
4. Study showed that SNAP23 was recruited to the close sites of lipid droplet (LD) in HCV-infected cells, implying that SNAP23 was required for HCV-induced LD enlargement and, HCV production. PMID: 27550144
5. A novel regulatory mechanism for SNAP23-dependent mast cell activation of T. vaginalis-secreted LTB4 involving surface trafficking of BLT1. PMID: 27795355
6. Study identified SNAP23 as a novel oncogene in Ovarian Cancer (OC). It is over-expressed in OC and could promote the proliferation, migration and invasion of OC in vitro. PMID: 27855700
7. Localization of SNAP23 was found in plasma membrane, lipid droplets and mitochondria of skeletal muscle. PMID: 26733245
8. these data suggest that SNAP23 is a key component of the endothelial SNARE machinery that mediates endothelial exocytosis. PMID: 26266817
9. Increased level of SNAP23-Syntaxin4-VAMP7 interaction correlates with decreased Syntaxin4 phosphorylation and trafficking of MT1-MMP to invadopodia during cellular invasion. PMID: 24807903
10. The association of Src, EGFR and beta1 integrin is dependent upon membrane traffic that is mediated by syntaxin13 (officially known as STX12) and SNAP23. PMID: 24496451
11. Data suggest SNAP23 and VAMP3 (vesicle-associated membrane protein 3) participate in interleukin-1beta-, interleukin-1 receptor-, calcium signaling-dependent secretion/exocytosis of interleukin-6 and tumor necrosis factor alpha from synoviocytes. PMID: 24373201
12. Results suggest that phagosomal SNAP-23 is one of the key players regulating the phagosomal environment in macrophages. PMID: 23087210
13. STX-3 and SNAP-23 are crucial for the release of all chemokines in mature human mast cells PMID: 21981832
14. Data show that knockdown of SNAP-23 inhibited the production of virus. PMID: 21768361
15. Introduction of the SNARE domain of SNAP-23 into neutrophils as an HIV transactivator of transcription (TAT) fusion protein significantly inhibits exocytosis of neutrophil granule subsets without altering signal transduction pathway activation. PMID: 21642540
16. SNAP23 and SNAP25 palmitoylation is regulated by DHHC palmitoyl transferases PMID: 20519516
17. the importance of SNAP23 in the trafficking of matrix metalloproteinases during degradation of extracellular matrix substrates and subsequent cellular invasion PMID: 19910495
18. SNAP-23 and syntaxin-4 are expressed in human eosinophils and are likely candidates for association with VAMP-2 during docking, which is followed by exocytosis. PMID: 11842301
19. cleaved by calpain in activated platelets PMID: 12121992
20. CFTR-mediated chloride currents are inhibited by introducing excess SNAP-23 into HT29-Cl.19A epithelial cells. PMID: 12209004
21. A synaptosome-associated protein of 23 kDa(SNAP23), the ubiqitously expressed homologue of SNAP25, has been shown to interact directly with ubiquitous kinesin heavy chain (uKHC). PMID: 12475239
22. Palmitoylated peptides from the cysteine-rich domain of this protein cause membrane fusion depending on peptide length, position of cysteines, and extent of palmitoylation. PMID: 12551899
23. an examination of the homotetrameric structure of the N-terminal domain of this protein PMID: 12556468
24. studies show that synaptosomal-associated protein-23 is phosphorylated in platelets during cell activation through a protein kinase C-related mechanism at two or more sites PMID: 12930825
25. Because SNAP-23 plays a central role in SNAREs complex formation, it was chosen to examine possible functional implications of the SNARE system in plasma cell Ig secretion. PMID: 16272324
26. expression of SNAP-23 and syntaxin-2 on the extracellular surface of the platelet plasma membrane. PMID: 17485553
27. SNAP-23 is not essential for constitutive exocytosis of secreted alkaline phosphatase PMID: 17825825
28. The expression of the SNARE protein SNAP-25 and its cellular homologue SNAP-23, as well as syntaxin1 and VAMP (vesicle-associated membrane protein) in samples of normal parathyroid tissue, chief cell adenoma, and parathyroid carcinoma, was examined. PMID: 18457912
29. Overexpression of a dominant negative SNAP-23 mutant suppressed expression of P-selectin, CD40L, CD41, CD61, release from dense granules and platelet aggregation PMID: 18665925
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亞細胞定位：

Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note=Mainly localized to the plasma membrane.
蛋白家族：

SNAP-25 family
組織特異性：

Ubiquitous. Highest levels where found in placenta.
數據庫鏈接：

HGNC: 11131

OMIM: 602534

KEGG: hsa:8773

STRING: 9606.ENSP00000249647

UniGene: Hs.511149

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