Phospho-RPA2 (T21) Recombinant Monoclonal Antibody

說(shuō)明書

中文名稱：

磷酸化-RPA2 (T21)重組抗體
貨號(hào)：

CSB-RA020089A21phHU
規(guī)格：

￥1320
圖片：
Immunofluorescence staining of Hela cells with CSB-RA020089A21phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA020089A21phHU Phospho-RPA2 (T21) Recombinant Monoclonal Antibody 是一種針對(duì)復(fù)制蛋白A2（RPA2）第21位蘇氨酸磷酸化修飾的特異性抗體。RPA2作為DNA結(jié)合復(fù)合物的核心亞基，在DNA復(fù)制、修復(fù)及損傷應(yīng)答中發(fā)揮關(guān)鍵作用，其T21位點(diǎn)的磷酸化修飾與DNA損傷信號(hào)傳導(dǎo)和基因組穩(wěn)定性調(diào)控密切相關(guān)。該抗體通過(guò)重組單克隆技術(shù)制備，經(jīng)ELISA和免疫熒光（IF）嚴(yán)格驗(yàn)證，可精準(zhǔn)識(shí)別內(nèi)源性磷酸化RPA2，在IF實(shí)驗(yàn)中推薦使用1:20至1:200的稀釋范圍，展現(xiàn)出高信噪比與重復(fù)性。適用于探究DNA代謝異常相關(guān)機(jī)制，如電離輻射或化療藥物誘導(dǎo)的復(fù)制應(yīng)激響應(yīng)、細(xì)胞周期檢查點(diǎn)調(diào)控及同源重組修復(fù)過(guò)程的分子動(dòng)態(tài)研究，為腫瘤生物學(xué)、遺傳毒性分析等基礎(chǔ)科研領(lǐng)域提供可靠工具。
Uniprot No.：

P15927
基因名：

RPA2
別名：

60S acidic ribosomal protein P1 antibody; AA409079 antibody; AI325195 antibody; AU020965 antibody; ik:tdsubc_2g1 antibody; M(2)21C antibody; MGC137236 antibody; OTTHUMP00000004008 antibody; p32 antibody; p34 antibody; RCJMB04_6d17 replication protein A2; 32kDa antibody; REPA2 antibody; Replication factor A protein 2 antibody; Replication protein A 32 kDa subunit antibody; Replication protein A 32kDa subunit antibody; Replication protein A 34 kDa subunit antibody; Replication protein A antibody; Replication Protein A2 (32kDa) antibody; Replication protein A2 antibody; Replication protein A2; 32kDa antibody; RF-A protein 2 antibody; Rf-A2 antibody; RFA antibody; RFA2_HUMAN antibody; RP-A p32 antibody; RP-A p34 antibody; RP21C antibody; RPA 2 antibody; RPA 32 antibody; RPA antibody; Rpa2 antibody; RPA32 antibody; RPA34 antibody; RpLP1 antibody; RpP2 antibody; xx:tdsubc_2g1 antibody; zgc:109822 antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human Phospho-RPA2 (T21)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

3B2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IF

推薦稀釋比：

Application	Recommended Dilution
IF	1:20-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
基因功能參考文獻(xiàn)：
1. RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation. PMID: 28107649
2. Single point mutations in the RPA32 subunit of RPA that abolish interaction with RFWD3 also inhibit interstrand crossling repair, demonstrating that RPA-mediated RFWD3 recruitment to stalled replication forks is important for ICL repair. PMID: 28575657
3. The authors show that Vpr can form a trimolecular complex with UNG2 and RPA32 and the positive effect of UNG2 and RPA32 on the reverse transcription process leading to optimal virus replication and dissemination between the primary target cells of HIV-1. PMID: 27068393
4. RPA32 phosphorylation regulates replication arrest, recombination, late origin firing, and mitotic catastrophe PMID: 24819595
5. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. PMID: 25113031
6. Conserved motifs are required for RPA32 binding the the N-terminus of SMARCAL1. PMID: 24910198
7. study reports the characterization of the RPA32C-SMARCAL1 interface at the molecular level; implications of results are discussed with respect to the recruitment of SMARCAL1 and other DNA damage response and repair proteins to stalled replication forks PMID: 24730652
8. study concludes RPA2 expression is translationally regulated via internal ribosome entry site and by eIF3a and that this regulation is partly accountable for cellular response to DNA damage and survival. PMID: 23393223
9. this study has explored the role of RPA32 phosphorylation at CDK and ATR sites and propose that phosphorylation of the RPA32 subunit is dispensable for checkpoint activation induced by replication stress with aphidicolin. PMID: 23047005
10. 4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2 PMID: 22684010
11. Data show that the R88C variant impairs binding of the R88C variant impairs binding of uracil-DNA glycosylase UNG2 to replication protein A RPA2. PMID: 22521144
12. Replication protein A1, replication protein A2, and cyclins D2 and D3 seem to have a parallel role in the promotion of cell cycle in astrocytic tumors being implicated in the malignant progression of these neoplasms. PMID: 21496876
13. RPA2 up-regulation may be involved in the growth and/or survival of BRCA1 tumor cells and useful in immunohistochemical discrimination of triple-negative BRCA1 tumors. PMID: 21137066
14. At the subunit level, 13 proteins out of 30 examined may interact with RPA2. PMID: 20679368
15. data suggest that RPA2 hyperphosphorylation plays a critical role in maintenance of genomic stability and cell survival after a DNA replication block via promotion of homologus recombination PMID: 20130019
16. Data suggest that PP4-mediated dephosphorylation of RPA2 is necessary for an efficient DNA-damage response. PMID: 20154705
17. RPA32, critical for cell proliferation and maintenance of genome stability, are markedly down-regulated, Data hypothesized that their DNA-related functions could be partially limited in TRAIL-resistant HL-60 cells. PMID: 19834905
18. Phosphorylation of the RPA2 subunit is observed after exposure of cells to ionizing radiation (IR) and other DNA-damaging agents, which implicates the modified protein in the regulation of DNA replication after DNA damage or in DNA repair. PMID: 11731442
19. RPA2 binds to menin and has a role in multiple endocrine neoplasia PMID: 12509449
20. C-terminal domain of hRPA32 subunit (RPA32C) facilitates initiation of SV40 replication. PMID: 15793585
21. in response to UV-induced DNA damage, ATR rapidly phosphorylates RPA2, disrupting its association with replication centers in the S-phase and contributing to the inhibition of DNA replication PMID: 17035231
22. Determination at single-nucleotide resolution the relative positions of the single-stranded DNA with interacting intrinsic tryptophans of RPA32. PMID: 17583916
23. RPA phosphorylation facilitates chromosomal DNA repair. PMID: 17928296
24. RPA32 is extensively phosphorylated after the induction of EBV lytic replication. Rad51 and RPA32 are necessary for the completion of EBV lytic infection. PMID: 19386720
25. The N-terminus of RPA1 and phosphorylation of RPA2 regulate RPA interactions with the MRE11-RAD50-NBS1 (MRN) complex and are important in the response to DNA damage. PMID: 19586055
26. mitotic phosphorylation of RPA2 starts at the onset of mitosis, and dephosphorylation occurs during late cytokinesis. PMID: 19671522
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亞細(xì)胞定位：

Nucleus. Nucleus, PML body.
蛋白家族：

Replication factor A protein 2 family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 10290

OMIM: 179836

KEGG: hsa:6118

STRING: 9606.ENSP00000363021

UniGene: Hs.79411

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