Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Recombinant Monoclonal Antibody
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中文名稱：磷酸化-PAK1/PAK2/PAK3 (S144+S141+S139)重組抗體
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貨號：CSB-RA017407A144phHU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in A549 whole cell lysate(treated with EGF or not)
All lanes Phospho-PAK1/PAK2/PAK3 antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 KDa
Observed band size: 65 KDa -
IHC image of CSB-RA017407A144phHU diluted at 1:100 and staining in paraffin-embedded rat brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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其他：
產品詳情
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產品描述：CSB-RA017407A144phHU重組單克隆抗體可特異性識別PAK1/PAK2/PAK3蛋白在S144、S141、S139位點的磷酸化修飾，該位點位于PAK激酶的自抑制結構域，其磷酸化狀態與PAK家族蛋白的激活及下游信號傳導密切相關。經多平臺驗證，本抗體在Western Blot實驗中可靈敏檢測多種哺乳動物細胞和組織樣本中內源性磷酸化PAK蛋白，推薦稀釋比例為1:500-1:5000；在免疫組化（IHC）實驗中能清晰呈現目標蛋白在石蠟包埋組織切片中的亞細胞定位，推薦稀釋比例為1:50-1:200。該抗體適用于研究PAK信號通路在細胞運動、腫瘤微環境調控以及神經突觸可塑性等領域的分子機制，尤其適用于探究細胞骨架重構、侵襲轉移等生物學過程中PAK激酶的動態磷酸化調控。采用重組單克隆技術制備，具備高批次穩定性和特異性，可為細胞功能研究和疾病相關蛋白互作分析提供可靠工具。
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Uniprot No.：
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human Phospho-PAK1/PAK2/PAK3 (S144+S141+S139)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：3H12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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