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中文名稱：磷酸化-MAPK3 (T202/Y204) + MAPK1 (T185/Y187)重組抗體
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貨號：CSB-RA013456A204phHU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in Jurkat whole cell lysate, Hela whole cell lysate, A549 whole cell lysate(treated with EGF or not)
All lanes Phospho-MAPK3 antibody at 0.95μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
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其他：
產品詳情
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產品描述：CSB-RA013456A204phHU重組單克隆抗體是針對磷酸化MAPK3（T202/Y204）與MAPK1（T185/Y187）雙位點的特異性檢測工具。該抗體靶向MAPK信號通路中ERK1/ERK2蛋白的關鍵激活位點，這些磷酸化修飾在細胞增殖、分化及應激響應等過程中發揮核心調控作用。經嚴格驗證，本品在Western Blot（WB）實驗中展現優異性能，可特異性識別內源性磷酸化ERK1/ERK2蛋白，推薦使用稀釋度范圍為1:500至1:5000，且在優化條件下呈現清晰的單一檢測條帶。同時支持ELISA平臺檢測，為研究人員提供多重實驗選擇。適用于探究生長因子刺激、氧化應激或藥物干預下MAPK通路激活狀態的動態變化，在腫瘤生物學、神經退行性疾病模型及藥物靶點機制研究中具有重要應用價值。本產品采用重組表達技術制備，具備高批次間穩定性和低交叉反應性特點，嚴格適用于科學研究領域。
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Uniprot No.：
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human Phospho-MAPK3 (T202/Y204) + MAPK1 (T185/Y187)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：4F6
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻
- Maternal postnatal early overfeeding induces sex-related cardiac dysfunction and alters sexually hormones levels in young offspring LA Ferreira,Journal of nutritional biochemistry,2023
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