Phospho-LAT (Y191) Recombinant Monoclonal Antibody
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中文名稱：磷酸化-LAT (Y191)重組抗體
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貨號：CSB-RA012767A191phHU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in:Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate(treated with EGF or Pervanadate)
All lanes: Phospho-LAT antibody at 2.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 KDa
Observed band size: 38 KDa -
IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of Hela cells with CSB-RA012767A191phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). -
Immunoprecipitating Phospho-LAT in A549 whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of CSB-RA012767A191phHU in A549 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA012767A191phHU(3μg)+ A549 whole cell lysate(1mg)
Lane 3: A549 whole cell lysate (20μg)
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其他：
產品詳情
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產品描述：CSB-RA012767A191phHU 磷酸化LAT（Y191）重組單克隆抗體是專為檢測LAT蛋白酪氨酸191位點磷酸化狀態設計的高特異性試劑。該抗體靶向T細胞受體信號通路中的關鍵接頭蛋白LAT，其Y191磷酸化位點對激活PLCγ1介導的鈣信號及免疫突觸形成具有重要作用。經嚴格驗證，該抗體在Western Blot中可清晰識別內源性磷酸化LAT蛋白，在免疫組化（IHC）和免疫熒光（IF）實驗中能精準定位細胞膜及胞內信號復合物的磷酸化位點，同時適用于免疫沉淀（IP）分析蛋白相互作用。推薦工作濃度覆蓋WB（1:500-1:5000）、IHC（1:50-1:200）、IF（1:20-1:200）及IP（1:200-1:1000），并支持ELISA檢測。憑借重組單克隆抗體的高批次穩定性，該產品可廣泛應用于T細胞活化機制研究、免疫相關疾病模型構建、腫瘤微環境免疫調控分析等基礎科研領域，為探索免疫信號網絡及藥物靶點驗證提供可靠工具。
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Uniprot No.：
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基因名：
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別名：LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human Phospho-LAT (Y191)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：2E4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 IF 1:20-1:200 IP 1:200-1:1000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
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基因功能參考文獻：
- Plasma membrane LAT activation precedes vesicular recruitment defining two phases of early T-cell activation. PMID: 29789604
- LAT1 overexpression represents a negative prognostic marker that has been linked to tumor grade, proliferating potential and angiogenesis and mediates intracellular transport of anticancer agents. PMID: 28815339
- The dimerization-dependent, cooperative binding of the growth factor receptor-bound protein 2 family to LAT may increase antigen receptor sensitivity by reducing signalosome formation at incompletely phosphorylated linker of activated T cells LAT molecules, thereby prioritizing the formation of complete signalosomes. PMID: 28951535
- Nur77 suppresses CD4(+) T cell proliferation and uncover a suppressive role for Irf4 in TH2 polarization; halving Irf4 gene-dosage leads to increases in GATA3(+) and IL-4(+) cells. PMID: 28538176
- LAT and phospholipase C-gamma dephosphorylation by SHP-1 inhibits natural killer cell cytototoxicity PMID: 27221712
- Study examined molecular mobility within LAT:Grb2:SOS assemblies on supported membranes by single-molecule tracking. Trajectory analysis reveals a discrete temporal transition to subdiffusive motion below a characteristic timescale, indicating that the LAT:Grb2:SOS assembly has the dynamical structure of a loosely entangled polymer. PMID: 29045874
- Here we report that phosphotyrosine-mediated assembly of adaptor protein LAT networks yields two distinct kinetic species of the Ras activator SOS PMID: 27370798
- Overexpression of LAT is associated with microcephaly. PMID: 28965845
- LAT and SLP-76 are randomly dispersed throughout the clusters that form upon T cell receptor engagement. PMID: 27875277
- this study shows that inherited LAT deficiency should be considered in patients with combined immunodeficiency with T-cell abnormalities PMID: 27522155
- This is the first report of a LAT-related disease in humans, manifesting by a progressive combined immune deficiency with severe autoimmune disease. PMID: 27242165
- Data show that LAT1 plays an important role in regulating the uptake of essential amino acids such as leucine into endometrial cancer cells. Increased ability of BCH compared to LAT1 shRNA at inhibiting Ishikawa spheroid area suggests that other LAT family members may also contribute to cell growth. PMID: 27486861
- Data indicate that the T cell-specific adaptor protein (TSAd) SH2 domain interacts with CD6 antigen and linker for activation of T cells protein (LAT) phosphotyrosine (pTyr) peptides. PMID: 27896837
- High expression of LAT1 and ASCT2 correlates with metastasis and invasion in esophageal squamous cell carcinoma. PMID: 26936531
- IFT20 is required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes. PMID: 26715756
- Data show that the palmitoylation mutation of linker for activation of T cells (LAT)attenuated the signal transduction induced by glycosylphosphatidylinositol-anchored CD59 antigen in T cells. PMID: 26271970
- HSV-1-encoded Us3 protein interrupted TCR signaling and interleukin-2 production by inactivation of the linker for activation of T cells. PMID: 25907557
- Patients with severe aplastic anemia had increased levels of LAT and disturbed Th1-Th2 balance. PMID: 24673455
- LAT is a modulator of CD3zeta and ZAP-70 tyrosine phosphorylation. PMID: 24204825
- mutation of palmitoylation site of LAT-EGFP attenuated the signal transduction of CD59 in T cells PMID: 24200054
- findings show that L-type amino acid transporter 1 is a major transporter for essential amino acids into activated human T cells PMID: 24038088
- Histone hypoacetylation on LAT promoter could inhibit LAT expression and enhanced Th2 differentiation, while trichostatin A, a histone deacetylase inhibitor, promoted LAT expression and inhibited Th2 cytokine production PMID: 23360572
- LAT is required to maintain calcium homeostasis in T cells. PMID: 22998346
- A novel function and mechanism of TRAF6 action in the T cell receptor(TCR)-LAT signaling pathway indicates that LAT also plays an adapter role in TCR/CD28-induced activation of TRAF6. PMID: 23514740
- LAT located at the cell surface can be recruited efficiently to activation-induced microclusters within seconds of T cell receptor (TCR) engagement. PMID: 23487428
- LAT cleavage may act as a regulator of TCR-mediated activation of T-cells and not only as a transducer of cell death promoting stimuli. PMID: 23240581
- Expression of constitutively active Raf transgene enhances lymphoproliferation, indicating a role for the Ras-MAPK pathway in LAT-mediated autoimmune hyperproliferation. PMID: 22984075
- Nef employs a dual mechanism to disturb early TCR signaling by limiting the communication between LAT and SLP-76 PMID: 22802418
- Modeling and simulation of aggregation of membrane protein LAT with molecular variability in the number of binding sites for cytosolic Grb2-SOS1-Grb2 PMID: 22396725
- the segment comprising residues 112-126 of human LAT is required for its interaction with Lck. PMID: 22034845
- Activation of T cell antigen receptors resulted in the recruitment and phosphorylation of linker for activation of T cells (Lat) from subsynaptic vesicles. Studies of Lat mutants confirmed that recruitment preceded Lat phosphorylation. PMID: 21642986
- Data show that MAL regulates membrane order and the distribution of microtubule and transport vesicle docking machinery at the IS and, by doing so, ensures correct protein sorting of Lck and LAT to the cSMAC. PMID: 21508261
- PECAM-1-mediated inhibition of GPVI-dependent platelet responses result from recruitment of SHP-2-p85 complexes to tyrosine-phosphorylated PECAM-1, which diminishes the association of PI3K with activatory signaling molecules Gab1 and LAT PMID: 20723025
- LAT recruits Src homology 2 domain-containing leukocyte protein of 76 kDa (SLP-76) following T-cell receptor ligation and Akt kinase phosphorylation in Jurkat cells, activating the phosphoinositide 3-hydroxykinase (PI3K) signaling cascade. PMID: 21282515
- Elevated expression of LAT1 is associated with squamous cell carcinoma of the lung. PMID: 19068093
- Results indicate for the first time that LAT promotes TCR signal initiation and suggest that this adaptor may contribute to maintain active Lck in proximity of their substrates. PMID: 21152094
- LAT ubiquitylation is a molecular checkpoint for attenuation of T-cell signaling. PMID: 21282648
- These results reveal a novel role of HSP90 as a positive regulator for expression of LAT gene in activated T cells. PMID: 21251717
- The adaptor LAT contains several conserved serine-based motifs, which are essential for proper signal transduction through the TCR. PMID: 20940326
- Studies indicate that LAT is present in membrane rafts and involved in signalling through a number of receptors other than the T cell receptor. PMID: 20875087
- A significant decrease of mRNA expression of LAT gene in T cells of asthmatic patients may be due to the up-regulation of its upstream regulatory factors Lck and ZAP-70. PMID: 18683785
- LAT displacement from lipid rafts is a molecular mechanism by which PUFAs inhibit T cell signaling PMID: 12029091
- Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav. PMID: 12186560
- LAT has a role in signal transduction for T cell activation and is stably associated with plasma membrane rafts PMID: 12515827
- interaction with open-active form of Lck in lipid rafts reveals a new mechanism for the regulation of Lck in T cells PMID: 12570875
- examination of synergistic assembly in T cell plasma membrane domains PMID: 12646565
- The T cell adapter molecule LAT is required for correct orientation of the microtubule-organizing center, as it moves toward the interface of the T cell and antigen-presenting cell. PMID: 12847255
- Results reveal the basis for preferential recognition of specific LAT sites by Gads. PMID: 15029250
- CD3/T cell receptor complex signaling to beta 1 integrins is defective in LAT-deficient Jurkat T cells, and can be restored with expression of wild-type LAT. PMID: 15100278
- LAB resembled a LAT molecule unable to bind phospholipase C-gamma1. PMID: 15153499
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相關疾病：Immunodeficiency 52 (IMD52)
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亞細胞定位：Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
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組織特異性：Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
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