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中文名稱：磷酸化-GYS1 (S641)重組抗體
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貨號：CSB-RA010078A641phHU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-GYS1 antibody at 1.12μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 KDa
Observed band size: 85 KDa -
Immunofluorescence staining of HepG2 cells with CSB-RA010078A641phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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其他：
產品詳情
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產品描述：Phospho-GYS1 (S641) Recombinant Monoclonal Antibody（Code: CSB-RA010078A641phHU）是一款高特異性抗體，靶向糖原合成酶1（GYS1）第641位絲氨酸磷酸化修飾位點。GYS1是糖原合成的關鍵限速酶，其活性通過S641等位點的磷酸化受到精確調控，這一修飾可抑制酶活性，進而影響細胞能量代謝穩態，在糖尿病、腫瘤等疾病的代謝重編程研究中具有重要意義。該抗體經ELISA、Western Blot（WB）及免疫熒光（IF）等多平臺驗證，可特異性識別內源性磷酸化GYS1蛋白，推薦稀釋度為WB 1:500-1:5000、IF 1:20-1:200，批次間一致性優異。適用于研究能量代謝調控機制、糖原合成通路異常相關疾病模型及藥物干預下的磷酸化動態變化，為探索細胞葡萄糖代謝、胰島素信號傳導及病理狀態下代謝適應性改變提供可靠工具。本產品僅用于科學研究，不適用于臨床診斷或其他用途。
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Uniprot No.：
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基因名：
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別名：Glycogen [starch] synthase antibody; Glycogen synthase 1 (muscle) antibody; Glycogen synthase 1 antibody; GSY antibody; GYS antibody; Gys1 antibody; GYS1_HUMAN antibody; muscle antibody
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human Phospho-GYS1 (S641)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：1D1
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:20-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻
- SARS-CoV-2 causes dysfunction in human iPSC-derived brain microvascular endothelial cells potentially by modulating the Wnt signaling pathway S Yamada,Fluids and barriers of the CNS,2024
產品評價
相關產品
靶點詳情
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功能：Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
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基因功能參考文獻：
- Insulin-stimulated glycogen synthase (GS) activity was completely ablated during hyperinsulinemic hypoglycemia, and catecholamine signaling via cAMP-dependent protein kinase (PKA) and phosphorylation of inhibiting sites on GS all increased. PMID: 28596236
- Overexpression of GYS1, MIF, and MYC is associated with adverse outcome and poor response to azacitidine in myelodysplastic syndromes and acute myeloid leukemia PMID: 25487600
- over-expression of muscle glycogen synthase (MGS)was detected in diabetic human kidney PMID: 25371328
- High glycogen synthase 1 expression is associated with myeloid leukemia. PMID: 25703587
- Data suggest that although COOH-terminal dephosphorylation is likely necessary for GS activation, protein kinase Akt-2- (Akt2)-dependent NH2-terminal dephosphorylation is site for "fine-tuning" insulin-mediated GS activation in skeletal muscle. PMID: 23321478
- Allosteric regulation and the relationship between phosphorylation and the kinetics of glycogen synthase. [Review] PMID: 23134486
- The present findings demonstrate that physical inactivity-induced insulin resistance in muscle is associated with lower content/activity of key proteins in glucose transport/phosphorylation and storage. PMID: 22403297
- Exercise unmaskd the effect associated with the GYS1 polymorphism, rendering carriers of this allele less susceptible to the protective effect of exercise on the risk of cardiovascular mortality. PMID: 17356695
- the M416V polymorphism of glycogen synthase 1 gene is not associated with insulin resistance in type 2 diabetes PMID: 12870167
- Phosphorylation at site 2 in the elderly and at site 3a + 3b in young twins had a genetic component. PMID: 15855312
- no nuclear export signal was identified in the sequence of the protein, the region comprising amino acids 555-633, which has an Arg-rich cluster involved in the allosteric activation is crucial for its nuclear concentration and aggregation. PMID: 15955076
- 3 siblings were identified with profound muscle glycogen deficiency and homozygous stop mutations in GYS1 PMID: 17928598
- Pioglitazone treatment improved insulin-stimulated glucose metabolism and glycogen synthase activity in PCOS. PMID: 18544618
- After overnight low muscle glycogen level and/or in response to exhausting exercise-induced glycogenolysis, GSY is associated with spherical structures at the I-band of sarcomeres. PMID: 19339242
- Dysregulation of glycogen synthase phosphorylation plays a major role in impaired insulin regulation of GS in obesity and type 2 diabetes mellitus. PMID: 19837931
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相關疾病：Muscle glycogen storage disease 0 (GSD0b)
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蛋白家族：Glycosyltransferase 3 family
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數據庫鏈接：
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