HDAC2 Recombinant Monoclonal Antibody
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中文名稱：HDAC2重組抗體
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貨號(hào)：CSB-RA949799A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: MCF-7 whole cell lysate, K562 whole cell lysate
All lanes: HDAC2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56, 52 kDa
Observed band size: 60 kDa -
IHC image of CSB-RA949799A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA949799A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of Hela Cells with CSB-RA949799A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA949799A0HU HDAC2重組單克隆抗體是針對(duì)組蛋白去乙?；?（HDAC2）開發(fā)的高特異性科研試劑，適用于多種實(shí)驗(yàn)場(chǎng)景。該抗體靶向的HDAC2作為表觀遺傳調(diào)控核心酶，通過介導(dǎo)組蛋白去乙?；瘏⑴c染色質(zhì)重塑、基因沉默及細(xì)胞周期調(diào)控，在神經(jīng)發(fā)育、腫瘤發(fā)生和代謝疾病研究中具有重要價(jià)值。經(jīng)嚴(yán)格驗(yàn)證，該抗體在Western Blot中可清晰檢測(cè)目標(biāo)蛋白表達(dá)，免疫組化（IHC）顯示組織特異性染色定位，免疫熒光（IF）實(shí)驗(yàn)呈現(xiàn)清晰的亞細(xì)胞核定位信號(hào)，推薦使用稀釋梯度為WB 1:500-1:5000、IHC 1:50-1:200、IF 1:20-1:200。其重組單克隆特性確保了實(shí)驗(yàn)結(jié)果的穩(wěn)定性和可重復(fù)性，適用于基因表達(dá)調(diào)控研究、表觀遺傳學(xué)機(jī)制探索、癌癥生物學(xué)分析以及神經(jīng)退行性疾病模型構(gòu)建等基礎(chǔ)科研領(lǐng)域，為研究者提供可靠的蛋白檢測(cè)工具。
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Uniprot No.：
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基因名：
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別名：Histone deacetylase 2 (HD2) (EC 3.5.1.98), HDAC2
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from human HDAC2
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：9H4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 IF 1:20-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.
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基因功能參考文獻(xiàn)：
- Results found that HDAC2 expression is up-regulated in patients with chronic diabetic foot ulcer and in high glucose induced endothelial progenitor cells. PMID: 30063937
- Comparative molecular docking studies of the lead RH01652 with class I HDACs (HDAC1, HDAC2, HDAC3, and HDAC8) shows higher binding affinity towards HDAC2. Thus, lead RH01652 could serve as template to design novel and potent inhibitor of HDAC2. PMID: 29932788
- human immortalized telencephalic/mesencephalic microglial cells reveal significant upregulation of HDAC2 in the presence of the potent microglial activator lipopolysaccharide PMID: 29803514
- we found that HDAC2 was the direct target of miR-31 by binding to 3'-UTR from the results of luciferase reporter assays, qRT-PCR, and western blotting. HDAC2 played an activation role in tumor growth, whose expression is upregulated and inversely associated with miR-31 levels PMID: 29333444
- data indicate that the obesity impacts on H4ac levels and that strenuous exercise leads to an enhanced chronic low-grade inflammation profile in obesity via an imbalance on H4ac/HDAC2. PMID: 29142617
- Knockdown of HDAC2 completely mimicked the effects of 1,25(OH)2D3 on PTEN gene expression. PMID: 28737824
- Muscle biopsies of chronic obstructive pulmonary disease significantly correlated with HDAC2 decrease compared to controls. PMID: 28526090
- With tissue microarrays of hepatocellular carcinoma (HCC) patients, we determined the prognostic values of the core genes in the network and found that RAD21, CDK1, and HDAC2 expression levels were negatively associated with overall survival for HCC patients. PMID: 28434945
- Combined treatment with the histone deacetylase inhibitors (HDACi) suberoylanilide hydroxamic acid plus 5-fluorouracil (5-FU) and oxaliplatin (Oxa) reduced the level of HDAC2 expression in HT-29 cells. PMID: 27283986
- miR-223 controls the expression of CX3CL1 by targeting HDAC2 in chronic obstructive pulmonary disease patients and mouse models of the disease. PMID: 26864305
- Data show that BRCA2 was required for HDAC2/3 association with acetylated BubR1 in nocodazole (Noc)-arrested cells. PMID: 28985013
- study defines two classes of HDAC2 targets in human cells, with a dependence of HDAC1 on HDAC2 at one class of targets, and distinguishes unique functions for HDAC2 PMID: 28982113
- This study demonstrated that the Expression of HDAC2 Transcript Is Reduced in Dorsolateral Prefrontal Cortex of Patients with Schizophrenia. PMID: 27959513
- The findings suggest that miR-455-3p plays a critical role during chondrogenesis by directly targeting HDAC2/8 and promoting histone H3 acetylation. PMID: 27638301
- report the interaction between CFTR and HDAC2, and its involvement in the development of Ph+ leukemia PMID: 28235656
- USP4 inhibits p53 and NF-kappaB through deubiquitinating and stabilizing HDAC2 PMID: 26411366
- HDAC2 controls ciliogenesis independently of Kras, which facilitates Aurora A expression. These studies suggest that HDAC2 is a novel regulator of primary cilium formation in PDAC cells. PMID: 28028031
- the current findings implicate the HDAC2/miR-101/AMPK pathway as a critical mediator of AD pathogenesis. These studies also highlight the importance of epigenetics in AD and provide novel therapeutic targets. PMID: 28202389
- HDAC2 upregulation is associated with hepatocellular carcinoma. PMID: 27342975
- Suggest that HDAC2 can trigger migration and invasion of non-small cell lung carcinoma cells by activating NF-kappaB to up-regulate fibronectin expression. PMID: 27665474
- KLF4 acts as a tumor suppressor or oncogene to activate or repress target gene transcription depending on its acetylation status, which is regulated by p21 and CK2 interaction-mediated HDAC2 phosphorylation PMID: 26729194
- Decreased HDAC2 expression is associated with cisplatin resistance in ovarian cancer. PMID: 26683361
- In cigarette smoke extract-exposed airway epithelial cells and macrophages, HDAC2 is excessively ubiquitinated and degraded in the proteasome attributed to low expression of USP17. PMID: 26617781
- The results imply that HDAC2 is involved in the transcriptional regulation of human odontoblasts in vivo. PMID: 22297573
- Endothelial-Mesenchymal Transition is initiated by recruitment of aberrantly phosphorylated DNMT1 to the RASAL1 CpG island promoter by HDAC2, causing aberrant promoter methylation and transcriptional suppression. PMID: 26815200
- Selective inhibition of HDAC2 in lung tumor cell causes survivin downregulation through activation of p53, which is mediated by downregulation of Mdm2. PMID: 25605253
- our study revealed that HDAC2 is overexpressed in colorectal cancer (CRC) cells; its knockdown can increase the sensitivity of CRC cells to doxorubicin via upregulation of ABCB1 PMID: 26846508
- Pancreatic adenocarcinoma patients with enhanced HDAC-1 and -6 expression showed significantly longer survival times compared to those with low expression, while a borderline association concerning HDAC-2 expression was noted. PMID: 26502922
- Lymphocyte senescence in COPD is associated with loss of HDAC2 in CD28nullCD8+ T and NKT-like cells. PMID: 26498345
- HDAC2 may not confer susceptibility to Schizophrenia in Han Chinese. PMID: 26063464
- In endothelial dysfunction, HDAC2 levels were reciprocally regulated by ectopic expression of NEDD8 and the de-NEDDylating enzyme SENP8. PMID: 25655932
- Aberrant overexpression of HDACs in basal cells of IPF lungs may contribute to the bronchiolisation process in this disease. Similarly, generation and apoptosis resistance of IPF fibroblasts are mediated by enhanced activity of HDAC enzymes. PMID: 26359372
- HDAC2 sumoylation is important for NF-kappaB-dependent gene expression and for the resistance of cell against stress PMID: 25704882
- HDAC2 inhibition reduces proliferation and induces apoptosis via the caspase-dependent pathway in human glioma cell lines, possibly by activating the p53 signaling pathway. PMID: 25523932
- Data indicate that that hstone deacetylase 2 (HDAC2) has a specific role in leukemogenesis. PMID: 25473896
- HDAC3 is an essential target to disrupt HIV-1 latency, and inhibition of HDAC2 may also contribute to the effort to purge and eradicate latent HIV-1 infection. PMID: 25136952
- BRG1/HDAC2 and beta-catenin constitute a manipulative apparatus at the transcription start site to play opposite but complementary roles in regulating hTERT expression. PMID: 25486475
- HDAC2 plays a central role in coupling lysine acetylation to synaptic plasticity and mediates many of the effects of HDAC inhibition in cognition and disease. PMID: 25492968
- Data suggest that p15RS (p15INK4b-related sequence) acts as an intrinsic transcriptional repressor for Wnt/beta-catenin-mediated gene transcription through recruiting HDAC2 histone deacetylase. PMID: 25697359
- Simultaneous loss of Hdac1 and Hdac2 resulted in loss of hematopoietic stem cells. PMID: 24763403
- our data suggested that cPA may have beneficial effects in inflammation-related cardiovascular disease by controlling HDAC2 regulation PMID: 25013374
- HDAC2 silencing in HCC cells also strongly inhibited PPARgamma signaling. PMID: 24958469
- inhibitory of cell growth by melittin might be led by HDAC2-mediated PTEN upregulation, Akt inactivation, and inhibition of the PI3K/Akt signaling pathways PMID: 24788349
- Osteocalcin levels were decreased, an effect induced at the transcriptional level, and were strongly correlated with inhibition of HDAC2. PMID: 24105979
- these results indicated that the potential of specific inhibition of HDAC2 by small molecular chemicals may lead to future therapeutic agents in human renal cancer treatment. PMID: 24390319
- The proliferation inhibition and cell cycle arrest mediated by downregulated HDAC2 expression may be tightly associated with the decrease of cyclin D1, cyclin E, and cdk2 proteins expression. PMID: 24965412
- PELP1 regulates tumor metastasis by controlling the expression and functions of the tumor metastasis suppressors miR-200a and miR-141 PMID: 23975430
- HDAC2 is a critical regulator of Arg2 expression and thereby endothelial nitric oxide and endothelial function. PMID: 24833798
- Data indicate prominent changes in urothelial cancer cell lines (UCC) were HDAC2 and/or HDAC8 up-regulation. PMID: 22944197
- our study has found no association between HDAC2/HDAC3 gene polymorphisms and schizophrenia in the Chinese Han population PMID: 23857786
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亞細(xì)胞定位：Nucleus. Cytoplasm.
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蛋白家族：Histone deacetylase family, HD type 1 subfamily
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組織特異性：Widely expressed; lower levels in brain and lung.
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