GPA33 Recombinant Monoclonal Antibody
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中文名稱：GPA33重組抗體
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貨號：CSB-RA245537A0HU
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規(guī)格：￥1320
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圖片：
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IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA245537A0HU GPA33重組單克隆抗體是針對人源糖蛋白A33（GPA33）設(shè)計的高特異性科研工具。GPA33作為一種跨膜糖蛋白，在正常胃腸道上皮細胞和結(jié)直腸癌等腫瘤組織中特異性表達，其參與細胞間粘附及信號調(diào)控的功能使其成為胃腸道腫瘤微環(huán)境研究的重要靶點。本抗體采用重組技術(shù)制備，經(jīng)ELISA和免疫組化（IHC）雙重驗證，在IHC應用中推薦使用1:50至1:200的稀釋比例，能清晰顯示石蠟包埋組織中靶蛋白的定位與表達水平，背景信號低且重復性優(yōu)異。該產(chǎn)品適用于胃腸道腫瘤病理學分析、腫瘤標志物篩選及細胞膜表面蛋白功能研究，可為探索GPA33在腫瘤發(fā)生發(fā)展中的分子機制、評估腫瘤組織特異性表達模式提供可靠支持，同時滿足體外實驗中對蛋白互作及信號通路研究的精準檢測需求。
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Uniprot No.：
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基因名：
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別名：Cell surface A33 antigen (Glycoprotein A33), GPA33
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反應種屬：Human
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免疫原：A synthesized peptide derived from human GPA33
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：4H4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應用范圍：ELISA, IHC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
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靶點詳情
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功能：May play a role in cell-cell recognition and signaling.
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基因功能參考文獻：
- A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID: 28226180
- A33 expression is directly correlated to colorectal tumor differentiation. PMID: 27272411
- Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID: 23230278
- Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID: 23294608
- The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID: 22623777
- Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID: 22623782
- cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID: 12114523
- Regulation of A33 antigen expression by GKLF. PMID: 12853980
- This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID: 18236042
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亞細胞定位：Membrane; Single-pass type I membrane protein.
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組織特異性：Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
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數(shù)據(jù)庫鏈接：
